) is a potent chemokine that plays an important role in the recruitment of macrophages. Although previous studies have demonstrated the importance of MCP-1 in the pathogenesis of diabetic nephropathy (DN) in terms of inflammation, the role of MCP-1 and its receptor (C-C chemokine receptor 2; CCR2) in extracellular matrix (ECM) accumulation under diabetic conditions has been largely unexplored. This study was undertaken to investigate the functional role of the MCP-1/CCR2 system in high glucoseinduced ECM (fibronectin and type IV collagen) protein expression in cultured mesangial cells (MCs). Mouse MCs were exposed to medium containing 5.6 mM glucose (NG), NGϩ24.4 mM mannitol (NGϩM), or 30 mM glucose (HG) with or without mutant MCP-1 (mMCP-1), CCR2 small interfering (si)RNA, or CCR2 inhibitor (RS102895). To examine the relationship between MCP-1 and transforming growth factor (TGF)-1, MCs were also treated with TGF-1 (2 ng/ml) with or without mMCP-1 or CCR2 siRNA. Transient transfection was performed with Lipofectamine 2000 for 24 h. Cell viability was determined by an MTT assay, mouse and human MCP-1 and TGF-1 levels by ELISA, and CCR2 and ECM protein expression by Western blotting. Transfections of mMCP-1 and CCR2 siRNA increased human MCP-1 levels and inhibited CCR2 expression, respectively. HG-induced ECM protein expression and TGF-1 levels were significantly attenuated by mMCP-1, CCR2 siRNA, and RS102895 (P Ͻ 0.05). MCP-1 directly increased ECM protein expression, and this increase was inhibited by an anti-TGF-1 antibody. In addition, TGF-1-induced ECM protein expression was significantly abrogated by the inhibition of the MCP-1/CCR2 system (P Ͻ 0.05). These results suggest that an interaction between the MCP-1/CCR2 system and TGF-1 may contribute to ECM accumulation in DN. diabetic nephropathy; monocyte chemoattractant protein-1; transforming growth factor-1; extracellular matrix MONOCYTES/MACROPHAGES ARE the principle inflammatory cells found in the diabetic kidney (6, 9, 29). These cells are extravasculated from the bloodstream through a process mediated by chemokines secreted from resident glomerular cells. Chemokines are a family of chemotactic cytokines that induce the migration of various cell types, and to date Ͼ40 chemokines have been identified (31). Among them, monocyte chemoattractant protein (MCP)-1 is the most extensively studied chemokine. In the kidney, MCP-1 is expressed in mesangial cells (MCs) and tubular epithelial cells (22,26) and is known to be involved in the pathogenesis of various renal diseases, including diabetic nephropathy. Previous studies have demonstrated that plasma MCP-1 levels are increased in type 1 diabetes with microalbuminuria (4) and that urinary levels of MCP-1 are also increased in accordance with the extent of albuminuria (1,20). In addition, it has been reported that glomerular MCP-1 expression is increased in experimental diabetic rats and that this increase is associated with the number of infiltrated monocytes in the glomeruli (5, 6). Moreover, an angio...