PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon1

Luo, Tian; Zhang, Xiaobo; Shao, Zongze; Xu, Xun

doi:10.1016/s0014-5793(03)00891-3

Cited by 161 publications

(96 citation statements)

References 37 publications

(36 reference statements)

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“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

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Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

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“…Other strategies with potential to combat WSSV infections include the induction of antiviral genes present in shrimp (Luo et al 2003), the application of synthetic antiviral peptides (Yi et al 2003), and the induction of a 'WSSV neutralizing factor' in shrimp using sublethal concentrations of WSSV (Venegas et al 2000, Wu et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Escobedo-Bonilla¹,

Audoorn²,

Wille³

et al. 2006

Dis. Aquat. Org.

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In the past, strategies to control white spot syndrome virus (WSSV) were mostly tested by infectivity trials in vivo using immersion or per os inoculation of undefined WSSV infectious doses, which complicated comparisons between experiments. In this study, the reproducibility of 3 defined doses (10, 30 and 90 shrimp infectious doses 50% endpoint [SID 50 ]) of WSSV was determined in 3 experiments using intramuscular (i.m.) or oral inoculation in specific pathogen-free (SPF) Litopenaeus vannamei. Reproducibility was determined by the time of onset of disease, cumulative mortality, and median lethal time (LT 50 ). By i.m. route, the 3 doses induced disease between 24 and 36 h post inoculation (hpi). Cumulative mortality was 100% at 84 hpi with doses of 30 and 90 SID 50 and 108 hpi with a dose of 10 SID 50 . The LT 50 of the doses 10, 30 and 90 SID 50 were 52, 51 and 49 hpi and were not significantly different (p > 0.05). Shrimp orally inoculated with 10, 30 or 90 SID 50 developed disease between 24 and 36 hpi. Cumulative mortality was 100% at 108 hpi with doses of 30 and 90 SID 50 and 120 hpi with a dose of 10 SID 50 . The LT 50 of 10, 30 and 90 SID 50 were 65, 57 and 50 hpi; these were significantly different from each other (p < 0.05). A dose of 30 SID 50 was selected as the standard for further WSSV challenges by i.m. or oral routes. These standardized inoculation procedures may be applied to other crustacea and WSSV strains in order to achieve comparable results among experiments.

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“…A number of genes involved in the shrimp innate immune system have been identified recently and characterized, including those involved in hemolymph coagulation (28,60), pattern recognition receptors (56), antibacterial proteins and peptides (3,15,58), and a limited number of proteins with antiviral properties (43,68). Despite significant advances in the identification and characterization of these genes and the wide range of physiological information available on the link between environmental stress and some indicators of immune vigor (e.g., total hemocyte counts, prophenoloxidase activity, and quantitation of reactive oxygen species), the influence of different stressors on gene expression in shrimp has been understudied.…”

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confidence: 99%

Stress-induced gene expression profiling in the black tiger shrimp Penaeus monodon

Vega

Hall

Wilson

et al. 2007

Physiological Genomics

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Cultured shrimp are continuously exposed to variable environmental conditions that have been associated with stress and subsequent outbreaks of disease. To investigate the effect of environmental stress on Penaeus monodon gene expression, a 3,853 random cDNA microarray chip was generated with clones originating from six stress-enriched hemocyte libraries generated by suppression subtractive hybridization and a normal hemocyte cDNA library. Changes in temporal gene expression were analyzed from shrimp exposed to hypoxic, hyperthermic, and hypoosmotic conditions; 3.1% of the cDNAs were differentially expressed in response to at least one of the environmental stressors, and 72% of the differentially expressed clones had no significant sequence similarity to previously known genes. Among those genes with high identity to known sequences, the most common functional groups were immune-related genes and non-long terminal repeat retrotransposons. Hierarchical clustering revealed a set of cDNAs with temporal and stress-specific gene expression profiles as well as a set of cDNAs indicating a common stress response between stressors. Hypoxic and hyperthermic stressors induced the most severe short-term response in terms of gene regulation, and the osmotic stress had the least variation in expression profiles relative to the control. These expression data agree with observed differences in shrimp physical appearance and behavior following exposure to stress conditions.

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PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon¹

Cited by 161 publications

References 37 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes

Stress-induced gene expression profiling in the black tiger shrimp Penaeus monodon

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