Pituranthos chloranthus Oil as an Antioxidant-Based Adjuvant Therapy against Cisplatin-Induced Nephrotoxicity

Abstract

The therapeutic outcome of cisplatin is limited due to its adverse side effects in normal tissues. Despite its potent antineoplastic effect, cisplatin is known by a relevant collateral action, for instance, acute renal failure. The aim of this study was to assess the effectiveness of Pituranthos chloranthus (PC) essential oil for contracting cisplatin-induced toxicity, in Balb/c mice. The standard mouse model of cisplatin-induced acute kidney injury (AKI), consisting of one intraperitoneal injection of cisplatin (20 mg/kg), was adopted. Mice were pretreated by intraperitoneal administration of PC (5 and 10 mg/Kg b.w) for one week. Cisplatin induced alteration in renal and liver functions, evidenced by increased serum biomarkers levels (creatinine, ALT, and AST). Significant mitigation of cisplatin-induced toxicity was confirmed by lowered levels of serum biomarkers and reduced DNA damage in liver and kidney. PC also restored the alterations in oxidative stress markers and proinflammatory cytokine IFN-γ level. Overall, this study provides, for the first time, that PC can be applied as an antioxidant-adjuvant treatment to mitigate cisplatin-induced renal failure.

The diminution and modulation role of water-soluble gallic acid-carboxymethyl chitosan conjugates against the induced nephrotoxicity with cisplatin

“…This led to several alterations in the membrane structure and activities of the cell and causes DNA damage, cytotoxicity, and eventually cell death 48 . Our data were consistent with previous work that indicated a significant increase in MDA levels among CDDP-challenged mice 49 . Additionally, the highly increased MDA was due to the CDDP-induced lipid peroxidation and damage of the plasma membrane exerting oxidative stress 48 .…”

The reversible and modulation role of water-soluble gallic acid-carboxymethyl chitosan conjugates against the induced nephrotoxicity with cisplatin

“…The current research showed that MDA levels in the kidney homogenates of rats administered intraperitoneal CDDP with 12 mg/kg body weight have been significantly upregulated with p < 0.001 in comparison to the control group (Table 2). These data were consistent with previous work that indicated a significant increase with a P-value of < 0.001 in MDA levels in CDDP-challenged mice 49 .…”

“…Our findings were comparable to the work that was done by Nabavi et al 50 which showed that antioxidant pre-treatments might improve NaF-induced nephrotoxicity in rats by direct scavenging of free radical and/or enhancing the endogenous antioxidant systems. GA showed antioxidant effects by directly free radicals scavenging or by indirectly increasing the antioxidant enzymes activity and expression 49,51 . In addition, GA@CMLMWC uptake exerted excellent biological activities in comparison to those of GA or CMLMWC alone.…”

“…The ability of CDDP to cross-link with purine bases in DNA, producing DNA damage in malignant cells, has been related to its genotoxic potential. This effect, however, is not tumor-specific and can harm normal cells 49 . The reported DNA damage caused by CDDP treatment in this study was in agreement with Hassan et al, 60 who found a significant increase in the tail length of DNA, tail intensity (DNA percent), and tail moment in CDDP-treated rats compared to controls when using the comet assay to determine the renal genotoxic potential of CDDP.…”

The Antioxidant Carrichtera annua DC. Ethanolic Extract Counteracts Cisplatin Triggered Hepatic and Renal Toxicities

“…Numerous traditionally used herbs were reported to ameliorate cisplatin’s adverse effects, especially hepato- and reno-toxicities, such as Dendropanax morbiferatea [ 19 ], ginger [ 20 ], marjoram [ 6 ], grape seed [ 21 ] and pomegranate rind [ 22 ]. Volatile oils of nigella [ 23 ], fennel [ 24 ] and Pituranthos chloranthus [ 25 ] have been proven to counteract cisplatin-induced renal and hepatic toxicities. Moreover, various phytochemicals demonstrated reno-and hepato-protective effects such as linalool [ 7 ], thymoquinone [ 26 ], cinnamic acid and its derivatives [ 10 , 27 , 28 , 29 ] as well as some flavonoids [ 30 , 31 , 32 ].…”