<i>Piper nigrum</i> seeds inhibit biofilm formation in <i>Pseudomonas aeruginosa</i> strains

Prabhu, Ashwini; Vipin, C; Kandal, Thasleema; Punchappady-Devasya, Rekha

doi:10.5958/0974-360x.2017.00707.7

Cited by 6 publications

(4 citation statements)

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“…The Antibiotic susceptibility test was carried out using the Kirby-Bauer disc diffusion method as described by Prabhu et al [14] and the Clinical Laboratory Standard Institute [12]. The standardized bacterial isolates were aseptically introduced on the surface of sterile Mueller Hinton agar (MHA) plates with the aid of a sterile swab stick using spread method.…”

Section: Antibiotic Susceptibility Testing Of Bacterial Isolates From...mentioning

confidence: 99%

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Ayesan,

Akinyele

2023

Microbes and Infectious Diseases

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Background: This study is aimed at identifying the bioactive components in Chromolaena odorata active against multiple antibiotics resistant bacteria (MARB) isolated from post-operative wound infections. Methods: A total of 69 septic wound samples were collected from patients (male and female) with clinically suspected postoperative wound infections. All methods were conducted using standard microbiological techniques. Results: Staphylococcus epidermidis was susceptible to gentamycin (10µg) at 24.50±0.50 mm, streptomycin (30µg) at 20.00±0.00 mm, ciprofloxacin (10µg) at 28.00±0.00 mm and amoxicillin (30µg) and are resistant to all other antibiotics tested. The 300 mg/ml concentration of the methanol crude extract had the highest zone of inhibition (ZOI) at 22.55±0.55 mm on Staphylococcus aureus and 22.30±0.30 mm ZOI was recorded on Enterococcus faecalis while the least ZOI at 6.45±0.25 mm on Streptococcus pyogenes. 12.5 mg/ml was the least inhibitory concentration on Klebsiella pneumoniae while 50 mg/ml was recorded as the highest minimum bactericidal concentration (MBC) on Staphylococcus epidermidis and Pseudomonas aeruginosa at 50 mg/ml. Conclusion: The identification and characterization of bioactive compounds from Chromolaena odorata leaves could help develop more effective antimicrobial treatments for the management of post-operative wound infections. Further research is needed to isolate and characterize the active bioactive chemical compounds in Chromolaena odorata responsible for the antimicrobial activity and to evaluate their safety and efficacy in clinical settings for the treatment of post-operative wound infections.

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Section: Antibiotic Susceptibility Testing Of Bacterial Isolates From...mentioning

confidence: 99%

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Ayesan,

Akinyele

2023

Microbes and Infectious Diseases

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“…From the analysis carried out and by comparison with the data reported in the literature, 2 was identified as 3-farnesyl-4-hydroxybenzoic acid [61], which has been previously reported in P. auritum, P. marginatum, P. obliquum, P. tricuspe and Dictyopteris divaricate [61][62][63][64]. This compound has been described in the literature for its antiparasitic (Plasmodium falciparum), antibacterial (Escherichia coli and Bacillus subtilis) properties and cell lines (MCF-7, H-460, and SF-268) [57,62], and for its ability to inhibit β-secretase-1, an important enzyme in the development of Alzheimer's disease [36].…”

Section: Phytochemical Study and Anti-qs And Anti-biofilm Activitymentioning

confidence: 99%

Antiquorum and Antibiofilm Activities of Piper bogotense C. DC. against Pseudomonas aeruginosa and Identification of Bioactive Compounds

Sierra-Quitian

Hernández-Moreno

Baquero

et al. 2023

Plants

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The present study describes the anti-biofilm and quorum sensing (QS) inhibitory potential of extracts and chemical constituents from Piper bogotense. Antibiofilm potential was determined through crystal violet assay against Pseudomonas aeruginosa, while QS inhibition efficacy was determined through violacein inhibition assay using Chromobacterium violaceum as a bacterial model. Additionally, this study reports the effects of the chemical constituents isolated in P. bogotense against various virulent factors associated with QS, such as the percentage decrease in pyocyanin, elastase, and protease production. The chemical study led to the isolation and identification of two prenylated benzoic acids (1 and 2) and a prenylated hydroquinone 3, of which compounds 1 and 2 are reported for the first time for P. bogotense. The ethanolic extract and the DCM fraction from P. bogotense stand out for reducing violacein production in C. violaceum, as well as the biofilm formation in P. aeruginosa. Compounds 2 and 3 stand out for having the lowest violacein production (43.8% and 68.3%), as well as the lowest production of virulence factors such as elastase (60.2% and 51.4%) and pyocyanin (39.7% and 33.2%). These results demonstrate the potential of P. bogotense components to be used as an alternative control against multidrug-resistant P. aeruginosa.

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“…The properties of extracts of seeds of P. nigrum, fruits of P. longum and aerial part of P. bogotense to inhibit violacein production in C. violaceum have been determined [20,27,30,31]. In addition, it is found that P. bogotense extract and seeds of P. nigrum can reduce biofilm formation [31,32]. It has also been reported that P. betle extract could inhibit pyocyanin production and swarming motility in P. aeruginosa [28,33,34].…”

Section: Introductionmentioning

confidence: 99%

Bioactive Compounds from P. pertomentellum That Regulate QS, Biofilm Formation and Virulence Factor Production of P. aeruginosa

Hernández-Moreno,

Pabón-Baquero,

Prieto-Rodriguez

et al. 2023

Molecules

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Pseudomonas aeruginosa is an opportunistic pathogen responsible for many nosocomial infections. This bacterium uses Quorum Sensing (QS) to generate antimicrobial resistance (AMR) so its disruption is considered a novel approach. The current study describes the antibiofilm and QS inhibitory potential of extract and chemical components from Piper pertomentellum. The methodo- logy included the phytochemical study on the aerial part of the species, the determination of QS inhibition efficacy on Chromobacterium violaceum and the evaluation of the effect on biofilm formation and virulence factors on P. aeruginosa. The phytochemical study led to the isolation and identification of a new piperamide (ethyltembamide 1), together with four known amides (tembamide acetate 2, cepharadione B 3, benzamide 4 and tembamide 5). The results indicated that the ethanolic extract and some fractions reduced violacein production in C. violaceum, however, only the ethanolic extract caused inhibition of biofilm formation of P. aeruginosa on polystyrene microtiter plates. Finally, the investigation determined that molecules (1–5) inhibited the formation of biofilms (50% approximately), while compounds 2–4 can inhibit pyocyanin and elastase production (30–50% approximately). In this way, the study contributes to the determination of the potential of extract and chemical constituents from P pertomentellum to regulate the QS system in P. aeruginosa.

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Piper nigrum seeds inhibit biofilm formation in Pseudomonas aeruginosa strains

Cited by 6 publications

References 0 publications

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Antiquorum and Antibiofilm Activities of Piper bogotense C. DC. against Pseudomonas aeruginosa and Identification of Bioactive Compounds

Bioactive Compounds from P. pertomentellum That Regulate QS, Biofilm Formation and Virulence Factor Production of P. aeruginosa

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