Pichia pastoris as a Versatile Cell Factory for the Production of Industrial Enzymes and Chemicals: Current Status and Future Perspectives

Abstract

With nearly three decades of development, Pichia pastoris (P. pastoris) has become a powerful eukaryotic protein expression system for the expression of thousands of proteins both on a laboratory and industrial scale. In addition, it has also been extensively used as a cell factory for the production of a variety of chemicals. This review summarizes the bottlenecks and solutions in achieving high-level secretory protein expression with P. pastoris and then outlines its applications on chemical production with an emphasis on its role as whole-cell biocatalyst. Furthermore, current challenges and future directions of this important system are also discussed.

Rationale‐based selection of optimal operating strategies and gene dosage impact on recombinant protein production in Komagataella phaffii (Pichia pastoris)

“…The lower values observed in maintenance coefficient could be expected since K. phaffii has been described as a robust system in terms of present lower maintenance requirements over other alternative platforms such as E. coli (Zhu et al , ). However, in this work, a relevant difference on m S has been described between SCC and MCC, specifically the difference is about one order of magnitude (0.0023 and 0.029 respectively).…”

Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia pastoris

“…P. pastoris has raised an increasing interest for production of recombinant proteins (Gasser et al, 2013;Zhu et al, 2019), and so far large numbers of proteins have been expressed successfully in P. pastoris (Bill et al, 2011;Ahmad et al, 2014;Lee et al, 2016;Karbalaei et al, 2020). Due to the particular advantages of P. pastoris, this expression system was implemented to produce FBG1 for pharmaceutical diosgenin production.…”

The secretome of Pichia pastoris in fed‐batch cultivations is largely independent of the carbon source but changes quantitatively over cultivation time

“…Komagataella spp .) is a methylotrophic yeast, which is commonly used as host platform for the production of recombinant secretory proteins of pharmaceutical (Weinacker, et al , ) and industrial interest (Rabert, et al , ; Love, et al , ; Zhu, et al , ). P. pastoris has been reclassified to the genus Komagataella in the recent years, which currently consists of seven species (including K. pastoris and K. phaffii ).…”