Physalis angulata concentrated ethanolic extract suppresses nociception and inflammation by modulating cytokines and prostanoids pathways

Abstract

Physalins are seco-steroids with a variety of pharmacological activities already described. In this study the pharmacological properties of a standardized concentrated ethanolic extract from Physalis angulata (CEEPA), rich in physalins B, D, F and G, were studied in models of pain and inflammation in mice. Inflammatory mediators were measured by radioimmunoassay and Real-Time PCR in mice paws after the CFA stimuli. Systemic administration of CEEPA produced antinociceptive effect on the writhing test and formalin test. In the writhing test, physalins B, D, F and G showed that the antinociceptive effect of CEEPA is more potent than that of these purified compounds. In addition, CEEPA reduced the levels of TNF-α, IL-1β, COX-2 and iNOS mRNA in the CFA-induced paw inflammation. Likewise, CEEPA decreased the TNF-α, IL-1β and PGE 2 paw levels. In conclusion, CEEPA induces antinociceptive and anti-inflammatory effects, with improved pharmacological potency relative to pure physalins, associated to modulation of cytokine and cyclooxygenase pathways.

Physalis angulata Linn. as a medicinal plant (Review)

“…P. angulata L. contains active ingredients that have medicinal properties. These active substances are: i) Physalin A in the roots, with antiparasitic properties ( 23 ), ii) physalin B in the whole plant, with anti-inflammatory, antiparasitic, antibacterial, anticancer and antifibrotic properties ( 10 , 16 , 17 , 27 , 29 , 31 , 37 , 43 ), iii) physalin D, F, G in the whole plant, with anti-inflammatory, antiparasitic and antibacterial properties ( 11 , 20 , 23 , 32 , 34 , 43 ), iv) physalin E in the whole plant, with anti-inflammatory and antiparasitic properties ( 23 ), v) physalin H in the root, with antiparasitic properties ( 20 , 31 ), vi) withangulatin A in the fruit, with antidiabetic properties ( 35 ) and vii) physangulatin A in the leaves and stems, with anti-inflammatory properties ( 35 ). The active substances in P. angulata L. are also presented in Table II and Fig.…”

Therapeutic Applications of Physalins: Powerful Natural Weapons

“…In addition, chemical synthesis to obtain physalins needs to be better explored, since the purification of physalins from natural sources is a time-consuming, costly and not environmentally friendly process that results in a low yield. Due to the fast growth of the plants, which are annual herbs, an approach that has been investigated is the use of a physalis extract concentrated in the physalins, which has shown both low toxicity as well as pharmacological effects ( Nogueira et al, 2013 ; Meira et al, 2015 ; Daltro et al, 2020 ; Do Espírito Santo et al, 2021 ).…”

Physalis angulata reduces the progression of chronic experimental periodontitis by immunomodulatory mechanisms

“…A total of sixty male C57BL/6 mice were randomly assigned to five experimental groups: naïve (untreated mice without periodontitis) (n = 12); vehicle (periodontitis-induced mice treated with saline + 5% DMSO) (n = 12); EEPA50 (periodontitis-induced mice treated with EEPA at 50 mg/kg) (n = 12); EEPA100 (periodontitis-induced mice treated with EEPA at 100 mg/kg) (n = 12); and nimesulide (periodontitis-induced mice treated with nimesulide at 25 mg/kg) (n = 12). The doses of EEPA were selected based on a previous study that has shown the antinociceptive and anti-inflammatory effects of this same extract in different experimental models in mice (Espírito-Santo et al, 2019). Nimesulide (Sigma-Aldrich), a nonsteroidal anti-inflammatory drug (NSAID) widely used in dentistry for treating inflammatory conditions (Krasniqi and Daci, 2017), was used as the gold standard drug.…”