Physalis alkekengiCarotenoidic Extract Inhibitor of Soybean Lipoxygenase-1 Activity

Abstract

The aim of this study was to evaluate the effect of the carotenoidic saponified extract of Physalis alkekengi sepals (PA) towards the lipoxygenase (LOX) oxidation of linoleic acid. Lipoxygenase activity in the presence of carotenoids, standard and from extract, was followed by its kinetic behaviour determining the changes in absorption at 234 nm. The standard carotenoids used were β-carotene (β-car), lutein (Lut), and zeaxanthin (Zea). The calculated enzymatic specific activity (ESA) after 600 s of reaction proves that PA carotenoidic extract has inhibitory effect on LOX oxidation of linoleic acid. A longer polyenic chain of carotenoid structure gives a higher ESA during the first reaction seconds. This situation is not available after 600 s of reaction and may be due to a destruction of this structure by cooxidation of carotenoids, besides the classical LOX reaction. The PA carotenoidic extract inhibiting the LOX-1 reaction can be considered a source of lipoxygenase inhibitors.

Metabolite Profiling, through LC-ESI/LTQOrbitrap/MS Analysis, of Antioxidant Extracts from Physalis alkekengi L.

“…On the other hand, the extracts obtained by decoction showed some differences with respect to EtOH:H 2 O extracts: in fact, they presented the same composition with regard to flavonoids, phenylpropanoids, organic acids, but were characterized by a low amount of physalins, and they were not characterized by the presence of carotenoids, identified in hydroalcoholic extracts. Previous studies have found that carotenoids in Physalis alkekengi L. fruit have good antioxidant effects and safety [32].…”

“…In addition to the presence of organic acids, p-coumaric acid (2), quinic acid (6), 1,3-dicaffeoylquinic acid (9), 4,5-dicaffeoylquinic acid (12), 3-caffeoylquinic acid (22), 5-caffeoylquinic acid (43), and caffeic acid (25) were revealed together with their derivatives, as suberic acid (4), feruloyl dihexoside (5), feruloyl hexoside (32), caffeoylglycerol (36), and coumaroyl dihexoside (2 isomers, 45 and 46). Coumaroyl and feruloyl glycosides largely contribute to the antioxidant activity of diverse plant materials [19].…”