<i>Phyllanthus urinaria</i>Induces Apoptosis in Human Osteosarcoma 143B Cells via Activation of Fas/FasL- and Mitochondria-Mediated Pathways

Wu, Hsien-Ming; Lin, Tsu-Kung; Kuo, Hsi-Kung; Huang, Yaling; Liou, Chia‐Wei; Wang, Pei‐Wen; Chuang, Jiin‐Haur; Huang, Sheng‐Teng

doi:10.1155/2012/925824

Cited by 22 publications

(17 citation statements)

References 41 publications

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“…These peaks were tentatively identified as phyllanthusiin U, B, C and chebulagic acid detected with [M-H] at m/z 924, 969, 925 and 953, respectively and previously identified in Phyllanthus urinaria [36, 37]. Peak 28 with a pseudomolecular ion [M-H] at m/z 933.06 and fragment ions at m/z 915, 631, 451 and 301 were in agreement with the fragmentation pattern attributed to castalagin [38].…”

Section: Discussionmentioning

confidence: 86%

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr

El-Naa

Zaghloul

et al. 2017

BMC Complement Altern Med

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Background Nymphaea alba L. represents an interesting field of study. Flowers have antioxidant and hepatoprotective effects, rhizomes constituents showed cytotoxic activity against liver cell carcinoma, while several Nymphaea species have been reported for their hepatoprotective effects. Leaves of N. alba have not been studied before. Therefore, in this study, in-depth characterization of the leaf phytoconstituents as well as its antioxidant and hepatoprotective activities have been performed where N. alba leaf extract was evaluated as a possible therapeutic alternative in hepatic disorders.MethodsThe aqueous ethanolic extract (AEE, 70%) was investigated for its polyphenolic content identified by high-resolution electrospray ionisation mass spectrometry (HRESI-MS/MS), while the petroleum ether fraction was saponified, and the lipid profile was analysed using gas liquid chromatography (GLC) analysis and compared with reference standards. The hepatoprotective activity of two doses of the extract (100 and 200 mg/kg; P.O.) for 5 days was evaluated against CCl4-induced hepatotoxicity in male Wistar albino rats, in comparison with silymarin. Liver function tests; aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (GGT) and total bilirubin were performed. Oxidative stress parameters; malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (TAC) as well as inflammatory mediator; tumour necrosis factor (TNF)-α were detected in the liver homogenate. Histopathological examination of the liver and immunohistochemical staining of caspase-3 were performedResultsFifty-three compounds were tentatively identified for the first time in N.alba leaf extract, where ellagitannins represent the main identified constituents. Nine hydrocarbons, two sterols and eleven fatty acids were identified in the petroleum ether extract where, palmitic acid and linolenic acids represented the major saturated and unsaturated fatty acid respectively. N.alba AEE significantly improved the liver function, oxidative stress parameters as well as TNF-α in addition to the amelioration of histopathological features of the liver and a profound decrease in caspase-3 expression.ConclusionThese results shed light on the hepatoprotective effect of N. alba that is comparable with that of silymarin. The antioxidant activities of N. alba extract in addition to the inhibition of crucial inflammatory mediator, as TNF-α, might be the possible hepatoprotective mechanisms.

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Section: Discussionmentioning

confidence: 86%

Profile of bioactive compounds in Nymphaea alba L. leaves growing in Egypt: hepatoprotective, antioxidant and anti-inflammatory activity

Bakr

El-Naa

Zaghloul

et al. 2017

BMC Complement Altern Med

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“…Both aqueous and methanolic extracts of P. urinaria whole plant inhibit A549 cell metastasis by suppressed invasion and migration of A549 cells through the ERK1/2 and hypoxia signaling pathways (Lee et al, 2013b ). The hot water extract from whole plants of P. urinaria induces apoptosis in human osteosarcoma 143B cells through the Fas receptor/ligand expression pathway (Wu et al, 2012 ). The same extract inhibits invasion and migration of another osteosarcoma cell line, Saos-2 cells through the ERK and Akt signaling pathways (Lu et al, 2013 ).…”

Section: Pharmacological Activities Of P Urinaria mentioning

confidence: 99%

A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L.

Geethangili

Ding

2018

Front. Pharmacol.

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The genus Phyllanthus (L.) is one of the most important groups of plants belonging to the Phyllantaceae family. Phyllanthus urinaria (L.) is an annual perennial herbal species found in tropical Asia, America, China, and the Indian Ocean islands. P. urinaria is used in folk medicine as a cure to treat jaundice, diabetes, malaria, and liver diseases. This review provides traditional knowledge, phytochemistry, and biological activities of P. urinaria. The literature reviewed for this article was obtained from the Web of Science, SciFinder, PubMed, ScienceDirect, and Google Scholar journal papers published prior to December 2017. Phytochemical investigations reveal that the plant is a rich source of lignans, tannins, flavonoids, phenolics, terpenoids, and other secondary metabolites. Pharmacological activities include anticancer, hepatoprotective, antidiabetic, antimicrobial, and cardioprotective effects. Thus, this present review summarizes the phytochemical constituents and their biological activities including biological studies on various crude extracts and fractions both in vitro and in vivo, and on clinical trial information about P. urinaria. This review compiles 93 naturally occurring compounds from P. urinaria along with their structures and pharmacological activities. The review is expected to stimulate further research on P. urinaria, and its pharmacological potential to yield novel therapeutic agents.

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“…Geraniin, quercetin 3-β-d-glucopyranoside, kaempferol 3-β-dglucopyranoside, isocorilagin, quercetin, kaempferol and rutin showed the strong antiproliferative activity. [113][114] Paris polyphylla Eight compounds were obtained from Paris polyphalla (Trilliaceae) and identified as Falcarindiol, β-ecdysterone and six known saponins. The dry rhizomes (5 kg) of P. polyphylla were refluxed three times with 70% EtOH (10L each) for 6 hours to afford ethanolic extract.…”

Section: Phyllanthus Rheediimentioning

confidence: 99%