Phleum pratense pollen starch granules induce humoral and cell‐mediated immune responses in a rat model of allergy

Motta, Alexandre; Peltre, Gabriel; Dormans, J. A. M. A.; Withagen, C. E. T.; Lacroix, Ghislaine; Bois, Frédéric Y.; Steerenberg, P. A.

doi:10.1111/j.1365-2222.2004.01872.x

Cited by 18 publications

(25 citation statements)

References 24 publications

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“…Allergen-bearing starch granules obtained upon contact of pollen with water have been shown to create an inhalable allergenic aerosol capable of triggering an early asthmatic response in an experimental in vitro study. Starch granules were shown to be recognised by pollen sensitised in rat serum and to trigger lymph node cell proliferation in these rats [29]. These data provide new arguments supporting the implication of grass pollen starch granules in thunderstormrelated asthma.…”

Section: Possible Mechanisms For Thunderstorm-related Asthmasupporting

confidence: 49%

A trans-disciplinary overview of case reports of thunderstorm-related asthma outbreaks and relapse

D’Amato¹,

Cecchi²,

Annesi‐Maesano³

2012

European Respiratory Review

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Section: Possible Mechanisms For Thunderstorm-related Asthmasupporting

confidence: 49%

A trans-disciplinary overview of case reports of thunderstorm-related asthma outbreaks and relapse

D’Amato¹,

Cecchi²,

Annesi‐Maesano³

2012

European Respiratory Review

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“…This suggests that lower concentrations of ozone might also induce significantly higher PCG release. PCG have been shown to contain allergens [10] and to be able to trigger an IgE-mediated response in rats [11] and asthmatic patients [16]. In previous studies, pollen-specific IgE ELISA inhibition experiments showed that 2.6 × 10 6 PCG contained as much allergen as in 100 µg of pollen.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, pollen-specific IgE ELISA inhibition experiments showed that 2.6 × 10 6 PCG contained as much allergen as in 100 µg of pollen. Knowing this, we then observed that for the same amount of allergens, PCG triggered a higher in vitro cellular response than intact pollen or pollen extract [10], indicating that PCG increased the bioavailability of pollen allergens. This may be explained by their particulate nature [17] and/or due to the more efficient presentation by antigen-presenting cells [18].…”

Section: Discussionmentioning

confidence: 99%

“…Due to their small size (3 µm), these granules can form a respirable aerosol capable of reaching the deep airways. Previously, we have shown that Phleum pratense pollen CG (PCG) contain allergens and can trigger a secondary allergic response in pollen-sensitized rats [10]. In addition, purified PCG were able to prime for specific IgE and T-cell responses in a rat model of allergy [11].…”

Section: Introductionmentioning

confidence: 99%

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Traffic-Related Air Pollutants Induce the Release of Allergen-Containing Cytoplasmic Granules from Grass Pollen

Motta

Marliere

Peltre³

et al. 2006

Int Arch Allergy Immunol

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146

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Background/Aim: Pollen cytoplasmic granules (PCG) are loaded with allergens. They are released from grass pollen grains following contact with water and can form a respirable allergenic aerosol. On the other hand, the traffic-related air pollutants NO₂ and O₃ are known to be involved in the current increase in the prevalence of allergic diseases via their adjuvant effects. Our objective was to determine the effects of air pollutants on the release of PCG from Phleum pratense (timothy grass) pollen. Methods:P. pratense pollen was exposed to several concentrations of NO₂ and O₃. The induced morphological damages were observed by environmental scanning electron microscopy, and the amount of PCG released from the pollen upon contact with water was measured. Results: The percentages of damaged grain were 6.4% in air-treated controls, 15% after treatment with the highest NO₂ dose (50 ppm) and 13.5% after exposure to 0.5 ppm O₃. In treated samples, a fraction of the grains spontaneously released their PCG. Upon subsequent contact with water, the remaining intact grains released more PCG than pollen exposed to air only. Conclusions: Traffic-related pollutants can trigger the release of allergen-containing granules from grass pollen, and increase the bioavailability of airborne pollen allergens. This is a new mechanism by which air pollution concurs with the current increase in the prevalence of allergic diseases.

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“…These granules, which contain most of the pollen major allergens, have been implicated in thunderstorm-associated asthma [21, 22]. They have also been shown to trigger primary and secondary allergic responses in pollen-sensitised Brown Norway rats [23, 24]. Recently, Zinnia pollen exposed to polluted air has been shown to trigger higher allergic responses in guinea pig [18].…”

Section: Introductionmentioning

confidence: 99%

Modifications of Phleum pratense Grass Pollen Allergens following Artificial Exposure to Gaseous Air Pollutants (O3, NO2, SO2)

Rogerieux

Godfrin²,

Sénéchal³

et al. 2007

Int Arch Allergy Immunol

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Background: Air pollution is frequently proposed as a potential cause of the increased incidence of allergy in industrialised countries. Our objective was to investigate the impact of the major gaseous air pollutants on grass pollen allergens. Methods: Timothy grass pollen was exposed to ozone (O₃), nitrogen dioxide (NO₂) and sulphur dioxide (SO₂) alone or in combination. Allergen contents were analysed by 2-dimensional immunoblot using grass pollen-sensitive patient sera. Results: For O₃-treated pollen, immunoblotting showed an acidification of allergens Phl p 1b, Phl p 4, Phl p 5 and Phl p 6 and an IgE recognition decrease in Phl p 1, Phl p 2, Phl p 6 and Phl p 13. NO₂ exposure induced a decrease in Phl p 2, Phl p 5b and Phl p 6 recognition, and SO₂ treatment induced a decrease in Phl p 2, Phl p 6 and Phl p 13 recognition. Moreover, samples treated with a mix of NO₂/O₃ or NO₂/SO₂ showed a higher decrease in allergen content, compared with samples treated with only one pollutant. The O₃ acidification was also observed with the NO₂/O₃ mix. Conclusion: Exposure of pollen to gaseous pollutants induced a decrease in allergen detection in pollen extracts. This decrease could be due to a mechanical loss of allergens from the altered pollen grains and/or post-translational modifications affecting allergen recognition by IgE.

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Phleum pratense pollen starch granules induce humoral and cell‐mediated immune responses in a rat model of allergy

Cited by 18 publications

References 24 publications

A trans-disciplinary overview of case reports of thunderstorm-related asthma outbreaks and relapse

A trans-disciplinary overview of case reports of thunderstorm-related asthma outbreaks and relapse

Traffic-Related Air Pollutants Induce the Release of Allergen-Containing Cytoplasmic Granules from Grass Pollen

Modifications of <i>Phleum pratense</i> Grass Pollen Allergens following Artificial Exposure to Gaseous Air Pollutants (O<sub>3</sub>, NO<sub>2</sub>, SO<sub>2</sub>)

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