<i>PgLOX6</i>encoding a lipoxygenase contributes to jasmonic acid biosynthesis and ginsenoside production in<i>Panax ginseng</i>

Rahimi, Shadi; Kim, Yujin; Zhang, Dabing; Yang, Deok-Chun

doi:10.1093/jxb/erw358

Cited by 29 publications

(13 citation statements)

References 50 publications

(67 reference statements)

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“…In addition, eight lipoxygenase were also up-regulated in PS. Previous studies indicated that lipoxygenase is the first key enzyme in the synthesis pathway of jasmonic acid, which plays an important role in the germination, growth, and stress resistance of plant seeds (Rahimi et al, 2016 ). GIGANTEA, a gene involved in circadian clock and phytochrome signaling (Cha et al, 2017 ), was down-regulated in PS (Table S5 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

et al. 2017

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Calcium not only serves as a necessary nutrient for plant growth but also acts as a ubiquitous central hub in a large number of signaling pathways. Free Ca2+ deficiency in the soil may cause early embryo abortion, which eventually led to abnormal development of peanut pod during the harvest season. To understand the mechanisms of Ca2+ regulation in pod development, transcriptome analysis of peanut gynophores and pods was performed by comparing the treatments between free Ca2+ sufficiency and free Ca2+ deficiency using Illumina HiSeq™ 2000. 9,903,082,800 nt bases are generated totally. After assembly, the average length of 102,819 unigenes is 999 nt, N50 is 1,782 nt. RNA-seq based gene expression profilings showed a large number of genes at the transcriptional level changed significantly between the aerial pegs and underground swelling pods under free Ca2+ sufficienct or deficiency treatments, respectively. Genes encoding key members of Ca2+ signaling transduction pathway, enzymes for hormone metabolism, cell division and growth, transcriptional factor as well as embryo development were highlighted. This information provides useful information for our further study. The results of digital gene expression (DGE) indicated that exogenous calcium might contribute to the development of peanut pod through its signal transduction pathway, meanwhile, promote the normal transition of the gynophores to the reproductive development.

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Section: Resultsmentioning

confidence: 99%

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

et al. 2017

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“…It was observed that the plant diets containing the soybean prooxidant enzyme lipoxygenase (LOX) (initiating various oxygenated compounds production) led to retarded Manduca sexta larval growth, the insect pest of plants from the family Solanaceae. Second, wounds induced by WCM feeding on barley leaves could stimulate releasing of fatty acids from membrane lipids such as linolenic acid and next LOX could convert linolenic acid to jasmonic acid and further volatile aldehydes can be produced (Mosblech et al 2009 ; Rahimi et al 2016 ). This would be consistent with the above postulated role of arginase in the production of polyamines and stimulated by them the emission of volatiles.…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Labudda

Tokarz

et al. 2020

Plant Cell Rep

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Key message Defence responses of cyst nematode and/or wheat curl mite infested barley engage the altered reactive oxygen species production, antioxidant machinery, carbon dioxide assimilation and photosynthesis efficiency. Abstract The primary aim of this study was to determine how barley responds to two pests infesting separately or at once; thus barley was inoculated with Heterodera filipjevi (Madzhidov) Stelter (cereal cyst nematode; CCN) and Aceria tosichella Keifer (wheat curl mite; WCM). To verify hypothesis about the involvement of redox metabolism and photosynthesis in barley defence responses, biochemical, photosynthesis efficiency and chlorophyll a fluorescence measurements as well as transmission electron microscopy were implemented. Inoculation with WCM (apart from or with CCN) brought about a significant suppression in the efficiency of electron transport outside photosystem II reaction centres. This limitation was an effect of diminished pool of rapidly reducing plastoquinone and decreased total electron carriers. Infestation with WCM (apart from or with CCN) also significantly restricted the electron transport on the photosystem I acceptor side, therefore produced reactive oxygen species oxidized lipids in cells of WCM and double infested plants and proteins in cells of WCM-infested plants. The level of hydrogen peroxide was significantly decreased in double infested plants because of glutathione–ascorbate cycle involvement. The inhibition of nitrosoglutathione reductase promoted the accumulation of S-nitrosoglutathione increasing antioxidant capacity in cells of double infested plants. Moreover, enhanced arginase activity in WCM-infested plants could stimulate synthesis of polyamines participating in plant antioxidant response. Infestation with WCM (apart from or with CCN) significantly reduced the efficiency of carbon dioxide assimilation by barley leaves, whereas infection only with CCN expanded photosynthesis efficiency. These were accompanied with the ultrastructural changes in chloroplasts during CCN and WCM infestation.

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“…Therefore, PLA and LOX , which mainly appear in the leaf, may make major contributions to the accumulation of total ginsenosides in the leaf. PgLOX6 , which encodes a lipoxygenase, contributes to JA biosynthesis and ginsenoside production in P. ginseng and provides a molecular tool for increasing ginsenoside production (Rahimi et al, 2016). However, the expression of JA-related genes in the xylem remains low, indicating that the xylem should be a component of the JA transport system, but is not central to JA biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Biogenic Synthesis and Spatial Distribution of Endogenous Phytohormones and Ginsenosides Provide Insights on Their Intrinsic Relevance in Panax ginseng

Chen

Liu

et al. 2019

Front. Plant Sci.

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Ginseng, the root and rhizome of Panax ginseng C. A. Mey., is a well-known and valuable traditional Chinese medicine. The pharmacological activities of ginseng are mainly attributed to the presence of ginsenosides, which are considered to be critical biomarkers for evaluating ginseng quality. The biosynthesis of triterpenes, which serve defensive functions in plants, is regulated by endogenous phytohormones that play key roles in growth and defense of plant populations. However, the role of major hormones that are closely related to secondary metabolism pathways in P. ginseng is poorly understood. To gain insight into their potential correlation, we performed a spatial synthesis analysis and studied the distribution of endogenous phytohormones and ginsenosides in different tissue regions of the entire P. ginseng plant. Gibberellins are growth hormones that accumulate in the fiber root. In contrast, abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), which are considered stress hormones, were predominantly found in the leaf and leaf peduncle. We observed a tissue-specific distribution of phytohormones consistent with the expression of genes involved in hormone biosynthesis that influenced ginsenoside synthesis and distribution. The aim of this study was to investigate the role of different endogenous phytohormones on triterpene metabolites in ginseng innate immunity.

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PgLOX6encoding a lipoxygenase contributes to jasmonic acid biosynthesis and ginsenoside production inPanax ginseng

Abstract: HighlightIn ginseng, jasmonic acid promotes expression of the biosynthetic genes for ginsenosides. PgLOX6 encodes a lipoxygenase that is required for biosynthesis of jasmonic acid and its overexpression increases ginsenoside levels.

Cited by 29 publications

References 50 publications

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella

Biogenic Synthesis and Spatial Distribution of Endogenous Phytohormones and Ginsenosides Provide Insights on Their Intrinsic Relevance in Panax ginseng

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