Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. Isolated from Clinical Specimens of Human Origin

Abstract

Three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. Phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within Clostridium cluster XIII. Two groups are most closely related to Peptoniphilus harei in the genus Peptoniphilus , and the other group is most closely related to Anaerococcus lactolyticus in the genus Anaerococcus . Based on the findings, three novel species, Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov., are proposed. The type strains of Peptoniphilus gorbachii sp. nov., Peptoniphilus olsenii sp. nov., and Anaerococcus murdochii sp. nov. are WAL 10418 T (= CCUG 53341 T = ATCC BAA-1383 T ), WAL 12922 T (= CCUG 53342 T = ATCC BAA-1384 T ), and WAL 17230 T (= CCUG 53340 T = ATCC BAA-1385 T ), respectively.

Peptoniphilus methioninivorax sp. nov., a Gram-positive anaerobic coccus isolated from retail ground beef

“…nov.; the type strain is NRRL B-23883 T (5DSM 22461 T ). (Murdoch, 1998;Ezaki et al, 2001;Song et al, 2007). In this study, an obligately anaerobic, Gram-positive coccal strain isolated from retail ground beef was characterized and a novel species, Peptoniphilus methioninivorax sp.…”

“…Members of this genus are found within Family XI Incertae Sedis (order Clostridiales, class Clostridia, phylum 'Firmicutes') (Vos et al, 2009) and what was referred to by Collins et al (1994) as 'Clostridium Cluster XIII'. Species of the genus Peptoniphilus have been found in the gastrointestinal and vaginal tracts of humans, as well as being associated with human clinical and veterinary wounds, abscesses or other infections (Murdoch, 1998;Ezaki et al, 2001;Song et al, 2007). In this study, an obligately anaerobic, Gram-positive coccal strain isolated from retail ground beef was characterized and a novel species, Peptoniphilus methioninivorax sp.…”

“…At the time of writing, the genus included seven species: Peptoniphilus asaccharolyticus (type species; Ezaki et al, 2001), Peptoniphilus harei (Ezaki et al, 2001), Peptoniphilus indolicus (Ezaki et al, 2001), Peptoniphilus ivorii (Ezaki et al, 2001), Peptoniphilus lacrimalis (Ezaki et al, 2001), Peptoniphilus olsenii (Song et al, 2010) and Peptoniphilus gorbachii (Song et al, 2010). Members of this genus are found within Family XI Incertae Sedis (order Clostridiales, class Clostridia, phylum 'Firmicutes') (Vos et al, 2009) and what was referred to by Collins et al (1994) as 'Clostridium Cluster XIII'.…”

Molecular detection of transcriptionally active bacteria from failed prosthetic hip joints removed during revision arthroplasty

“…Brevundimonas and Dermacoccus species have also been identified by 16S rRNA gene sequencing as being part of a diverse range of bacterial species found in aortic aneurysms [35]. Conventional microbiological culture methods have also isolated Sphingomonas species in advanced noma lesions, infections which are open to the environment [36] and Peptoniphilus species have been isolated from human clinical specimens [37,38].…”

“…Other bacterial genera identified by both culture-dependent and culture-independent methods were Virgibacillus [30], Brevundimonas [33][34][35], Sphingomonas [36] and Peptoniphilus [37,38]. Members of the Virgibacillus genus are environmental bacteria that have never been associated with human infections, whereas all the other genera identified by both methods have been isolated from human clinical specimens.…”