<i>Pepper mottle virus</i> Causing Disease in Chile Peppers in Southern New Mexico

Rodríguez-Alvarado, Gerardo; Fernández-Pavía, S. P.; Creamer, Rebecca; Liddell, C. M.

doi:10.1094/pdis.2002.86.6.603

Cited by 22 publications

(9 citation statements)

References 5 publications

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“…To our knowledge this is the first report of natural infection of Sambucus ebulus, Cichorium intybus, Conyza canadensis, Helliotropium europaeum, Lactuca serriola, Picris echioides and Scabiosa tennuiis by PVY (Schmelzer, 1967;De Bokx and Huttinga, 1981;Katis, 1984;Edwardson and Christie, 1997;Espino de Paz et al, 1997;Sˇkoric´et al, 2000;Fletcher, 2001;Kazinczi et al, 2002), of Fumaria officinalis by CMV Francki et al, 1979;Ford et al, 1988;Lorbeer, 1989, 1991;Crescenzi et al, 1993;Lavin´a et al, 1996;Fletcher, 2001;Rodriguez-Alvarado et al, 2002), and of Scabiosa tenuiis and Urtica dioica by AMV (Jaspars and Bos, 1980;Fletcher, 2001;Kazinczi et al, 2002). Some others such as Cirsium arvense, Cyperus rotundus, Mentha pulegium, Xanthium spinosum and X. strumarium for PVY, Amaranthus blitoides, Chondrila juncea, Malva neglecta and Mentha pulegium for CMV, as well as Cyperus rotundus and Picris echioides for AMV, were found infected for the first time by ELISA, but the virus detected was not readily recoverable in bioassays.…”

Section: Discussionmentioning

confidence: 76%

A survey of tobacco viruses in tobacco crops and native flora in Greece

Chatzivassiliou¹,

Efthimiou²,

Drossos

et al. 2004

European Journal of Plant Pathology

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The most important tobacco producing areas in Greece were surveyed for virus presence, from 1997 to 2000. Tobacco seedlings or plants showing virus-like symptoms were randomly collected from seedbeds or fields, respectively, and tested by ELISA, and/or mechanical inoculation onto indicator plants. Potato virus Y (PVY), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) were detected in all sampling areas, with TMV mainly found in oriental varieties. Tomato spotted wilt virus (TSWV) consisted a serious endemic virus in Northern Greece (Thrace, Central and Eastern Macedonia), whereas Alfalfa mosaic virus (AMV) was mainly found in regions, where alfalfa was cultivated in the vicinity of tobacco crops. Eggplant mottled dwarf virus (EMDV) was detected in several areas but always in very low incidence (<0.01%). Surveys were also conducted to assess the potential reservoir hosts of PVY, CMV and AMV among weeds collected from highly infected tobacco fields from 1998 to 2000. Among 3450 samples tested for PVY, plants from 17 species in 10 families were found infected. For CMV, 2891 weed samples were tested and 19 species in 12 families were positive. Assays for AMV infection were made on 961 samples and 12 species in 9 families were identified as hosts of this virus.

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Section: Discussionmentioning

confidence: 76%

A survey of tobacco viruses in tobacco crops and native flora in Greece

Chatzivassiliou¹,

Efthimiou²,

Drossos

et al. 2004

European Journal of Plant Pathology

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“…Coating buffer (1 ml) was added to each tube, and samples were reground. Indirect plate‐trapped ELISA was performed essentially as in the study of Rodríguez‐Alvarado et al. (2002).…”

Section: Methodsmentioning

confidence: 99%

Application of Vascular Puncture for Evaluation of Curtovirus Resistance in Chile Pepper and Tomato

Sedano¹,

Lam²,

Escobar³

et al. 2011

Journal of Phytopathology

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Curtoviruses cause severe damage to tomatoes and peppers. Functional field resistance to curtoviruses in these plants is desirable but difficult to produce and difficult to screen for because it is time-consuming and resistance could be achieved by developing resistance either to the virus or to insect feeding. To improve and speed curtovirus resistance testing in tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants, two puncture methods were developed and compared to leafhopper inoculation and feeding preference assays. The two puncture methods were adapted to introduce a modified Agrobacterium tumefaciens plasmid carrying a recombinant curtovirus into the meristem tissue of tomato plants and into newly germinated chile pepper seedlings. The puncture techniques were used to screen for resistance to curtoviruses in chile pepper and tomato breeding lines and varieties. Similarly, the peppers and tomatoes were assayed for curtovirus resistance using leafhopper inoculation and feeding preference, which was assessed by stylet sheath staining. Virus infection by puncture and leafhopper feeding was monitored using PCR and ELISA. ELISA was performed using an antibody to bacterially expressed coat protein. While pepper cvs Tabasco, NuMex Las Cruces cayenne and New Mexico 6-4 were infected using both puncture and leafhopper inoculation methods, New Mexico 6-4 had higher infection rates than the other two cultivars. Stylet sheath staining results suggest that leafhoppers prefer to feed on New Mexico 6-4 rather than Tabasco and NuMex Las Cruces cayenne. Eight tomato cultivars were infected using meristem removal injection inoculation. Three tomatoes cultivars (CVF-11, Saladmaster and Supersteak) were infected using leafhopper inoculation, although stylet sheath staining results suggested that the first two cultivars were not preferred by the insect vector. Our results suggest that puncture methods and leafhopper inoculation are successful in resistance screening, and both methods should be used as part of screening, because they assess different types of resistance.

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“…To our knowledge this is the first identification of PepMoV in Cuba. PepMoV has been reported affecting pepper growing areas of several states of the United States (Zitter, 1972; Rodriguez‐Alvarado et al , 2002) and Central America (Nelson & Wheeler, 1978). In 2003, it was found in C. annuum in Japan (Ogawa et al , 2003) and more recently in bell pepper in Taiwan (Cheng et al , 2011).…”

Section: Figurementioning

confidence: 99%