<i>Pediococcus pentosaceus</i>B49 from human colostrum ameliorates constipation in mice

Huang, Juqing; Li, Suyi; Wang, Qi; Guan, Xuefang; Qian, Lei; Li, Jie; Zheng, Yi; Lin, Bor‐Shyh

doi:10.1039/d0fo00208a

Cited by 38 publications

(19 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many P. pentosaceus strains isolated from different sources were proven to be linked with the human gastrointestinal tract [38]. In animal studies, P. pentosaceus strains have been reported that could improve acute liver failure, obesity, constipation, fatty liver, and intestinal inflammation [39][40][41][42][43]. In this study, we further revealed that a P. pentosaceus strain isolated from healthy children, CECT 8330, protected DSSinduced experimental colitis in mice as demonstrated by inhibition of weight loss, colon length shortening, and intestinal mucous damage.…”

Section: Discussionmentioning

confidence: 99%

Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice

Dong

Xiao

et al. 2022

J Transl Med

View full text Add to dashboard Cite

Background Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD). Therapies for targeting the microbiota may provide alternative options for the treatment of IBD, such as probiotics. Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice. Methods C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS. Weight, fecal conditions, colon length and histopathological changes were examined. ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio. Western blot was used to examine the tight junction proteins (TJP) in colonic tissues. Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles. Results P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening. P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice. P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue. P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella. Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively. The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS. Conclusions P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function. Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.

show abstract

Section: Discussionmentioning

confidence: 99%

Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice

Dong

Xiao

et al. 2022

J Transl Med

View full text Add to dashboard Cite

show abstract

“…It has been pointed out that Akkermansia was reduced in patients with IBD, indicating that it may have potential anti-inflammatory effects [65], which was consistent with our study. Ruminococcaceae UCG-014 is an SCFA producer with an anti-inflammatory effect, which can protect the intestinal barrier and ameliorate colitis [66]. Recent studies found that probiotics and prebiotics administration increased the levels of the Lachnospiraceae NK4A136 group caused by DSS, which plays a critical role in relieving colitis [67].…”

Section: Discussionmentioning

confidence: 99%

Alleviation Effects of Bifidobacterium animalis subsp. lactis XLTG11 on Dextran Sulfate Sodium-Induced Colitis in Mice

Wang

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD) is a chronic immune-related disease, which can occur through the dysfunction of the immune system caused by the imbalance of gut microbiota. Previous studies have reported the beneficial effects of Bifidobacterium on colitis, while the related mechanisms behind these effects have not been fully elucidated. The aim of our study is to investigate the alleviation effect of Bifidobacterium animalis subsp. lactis XLTG11 (B. lactis) on dextran sulfate sodium (DSS)-induced colitis and its potential mechanism. The results showed that B. lactis XLTG11 significantly decreased weight loss, disease activity index score, colon shortening, myeloperoxide activity, spleen weight, and colon tissue damage. Additionally, B. lactis XLTG11 significantly decreased the levels of pro-inflammatory cytokines and increased the level of anti-inflammatory cytokine. Meanwhile, high doses of B. lactis XLTG11 significantly up-regulated the expression of tight junction proteins and inhibited activation of Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MYD88)/nuclear factor-κB (NF-κB) signaling pathway. Furthermore, B. lactis XLTG11 increased the gut microbiota diversity and modulated gut microbiota composition caused by DSS. Moreover, Spearman’s correlation analysis also found that several specific gut microbiota were significantly correlated with colitis-related indicators. These results demonstrated that B. lactis XLTG11 can alleviate DSS-induced colitis by inhibiting the activation of the TLR4/MYD88/NF-κB signaling pathway, regulating inflammatory cytokines, improving intestinal barrier function, and modulating the gut microbiota.

show abstract

“…Treatment with P. pentosaceus PP8 derived from the gut of juvenile whiteleg shrimp promoted many functions in whiteleg shrimp, such as digestive enzyme activity, disease resistance, growth and immunity. Isolated from human colostrum, P. pentosaceus B49 relieved constipation not only by improving gastrointestinal motility and water and electrolyte absorption but also by promoting neurotransmitter transmission and the growth of beneficial shortchain fatty acid-producing bacteria [69]. Isolated from rumen fluid, P. pentosaceus S22, with low carbohydrate tolerance, exhibited good adaptability to the gastric juice environment.…”

Section: Referencesmentioning

confidence: 99%

“…The interactions between P. pentosaceus and the gut microbiota alone have been widely analysed, but not many studies have evaluated them by -omics approaches, such as transcriptomics, proteomics, metagenomics, and 16S rDNA/rRNA gene sequencing. For example, gastrointestinal peristalsis was partially improved by treatment with P. pentosaceus B49, as determined by analysing the transcriptomic outcome in the colon [69]. The cell adherence function of P. pentosaceus GS4 was proved to produce a 98 kDa surface layer protein, as determined by proteomic technology such as SDS-PAGE and size exclusion chromatography [123].…”

Section: Probiotic Applicationsmentioning

confidence: 99%

Pediococcus pentosaceus, a future additive or probiotic candidate

et al. 2021

View full text Add to dashboard Cite

Background Pediococcus pentosaceus, a promising strain of lactic acid bacteria (LAB), is gradually attracting attention, leading to a rapid increase in experimental research. Due to increased demand for practical applications of microbes, the functional and harmless P. pentosaceus might be a worthwhile LAB strain for both the food industry and biological applications. Results As an additive, P. pentosaceus improves the taste and nutrition of food, as well as the storage of animal products. Moreover, the antimicrobial abilities of Pediococcus strains are being highlighted. Evidence suggests that bacteriocins or bacteriocin-like substances (BLISs) produced by P. pentosaceus play effective antibacterial roles in the microbial ecosystem. In addition, various strains of P. pentosaceus have been highlighted for probiotic use due to their anti-inflammation, anticancer, antioxidant, detoxification, and lipid-lowering abilities. Conclusions Therefore, it is necessary to continue studying P. pentosaceus for further use. Thorough study of several P. pentosaceus strains should clarify the benefits and drawbacks in the future.

show abstract

Pediococcus pentosaceusB49 from human colostrum ameliorates constipation in mice

Abstract: P. pentosaceus B49 partly normalized the expression of genes related to intestinal peristalsis, water and electrolyte absorption and transport, while down-regulating the expression of pro-inflammatory and pro-oncogenic genes in constipated mice.

Cited by 38 publications

References 54 publications

Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice

Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice

Alleviation Effects of Bifidobacterium animalis subsp. lactis XLTG11 on Dextran Sulfate Sodium-Induced Colitis in Mice

Pediococcus pentosaceus, a future additive or probiotic candidate

Contact Info

Product

Resources

About