Pediococcus acidilactici P25 Protected Caenorhabditis elegans against Enterotoxigenic Escherichia coli K88 Infection and Transcriptomic Analysis of Its Potential Mechanisms

Tan, Keqin; Deng, Dun; Ma, Xianyong; Cui, Yiyan; Tian, Zhonghuan

doi:10.1155/2020/7340312

Cited by 11 publications

(13 citation statements)

References 81 publications

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“…C-type lectins have been reported to be involved in serum glycoprotein homeostasis, pathogen detection, and initiation of an immune response ( Mayer et al., 2017 ). UDP-glucuronyl transferase (UGT) and cytochrome P450 are involved in chemical detoxification and innate immune responses ( Simonsen et al., 2012 ; Tan et al., 2020 ). Tan et al.…”

Section: Resultsmentioning

confidence: 99%

Probiotic Lacticaseibacillus rhamnosus GG Increased Longevity and Resistance Against Foodborne Pathogens in Caenorhabditis elegans by Regulating MicroRNA miR-34

Yun

Ryu

Kang

et al. 2022

Front. Cell. Infect. Microbiol.

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In this study, we investigated the relation of probiotic activity of Lacticaseibacillus rhamnosus strain GG (LGG) and expression of microRNA to immune response and longevity in Caenorhabditis elegans host model. First, we evaluated the survival rate of C. elegans due to LGG exposure and bacterial colonization in the intestine. Next, the expression of mRNA and miRNA was analyzed in C. elegans exposure to LGG for 24 h using microarray. After exposure to LGG to C. elegans, colonized LGG was observed in the intestines of C. elegans and induced to extend lifespan. Moreover, persistent LGG in the intestine significantly enhanced the resistance of C. elegans exposed to both pathogenic bacteria and prolonged the lifespan of C. elegans. Transcriptome analysis indicated that LGG affected the expression levels of genes related to the innate immune response and upregulated the abundance of genes in multiple pathways of C. elegans, including Wnt signaling, TGF-beta signaling and mitogen-activated protein kinase (MAPK) pathways. In addition, qRT-PCR analysis confirmed that the expression of antibacterial genes was increased by LGG. Moreover, as the expression of microRNA miR-34 and immune-related pathways increased by exposure to LGG, the lifespan of C. elegans increased. However, in the miR-34 mutant C. elegans, the lifespan by LGG did not increase, so it was determined that miR-34 indirectly affects immune-related pathways. There was no significant difference in the expression of PMK-1 for LGG exposure in miR-34 mutants, suggesting that miR-34 may regulate PMK-1. In conclusion, we suggest that exposure of LGG to C. elegans enhances lifespan and resistance to food-borne pathogen infection by stimulating miR-34 and indirectly promoting PMK-1 activity.

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Section: Resultsmentioning

confidence: 99%

Probiotic Lacticaseibacillus rhamnosus GG Increased Longevity and Resistance Against Foodborne Pathogens in Caenorhabditis elegans by Regulating MicroRNA miR-34

Yun

Ryu

Kang

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Park et al (38) reported that probiotic L. fermentum strain JDFM216 stimulated the longevity and immune response of C. elegans through a nuclear hormone receptor (NHR) family and PMK-1 signaling (38). Pediococcus acidilactici P25 strain affected expression of the genes related to innate immune response and upregulated the abundance of transcripts in multiple pathways of C. elegans, including peroxisome, longevity, and MAPK pathways (42). Nevertheless, these reports have not yet identified immunomodulatory effects of Lactobacillus on the targeting sites downstream of the p38 MAPK and DAF/IGF pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Analyses of mutations in different genes associated with the responses have identified downstream proteins involved in pathogen defense, such as LYS (lysozyme) family, ABF ( Ascaris suum antibacterial factor) family, SPP (Caenopores are the saposin-like proteins) family, and C-type lectins family ( 29 , 30 ). However, the role of these antimicrobial peptides in response to bacterial infection is yet to be fully elucidated ( 7 , 14 , 42 ). In the present study, the transcription of genes that encode for antimicrobial peptides including abf-3, lys-7 , and spp-1 was all significantly upregulated in the WT nematode when it was exposed to DT104.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus Regulates Caenorhabditis elegans Cell Signaling to Combat Salmonella Infection

Zhou

Liu

et al. 2021

Front. Immunol.

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Salmonella typhimurium DT104 infection causes the death of Caenorhabditis elegans, which can be prevented by certain Lactobacillus isolates. However, the molecular mechanisms of both the host response to the infection and the protection by Lactobacillus are largely unclear. The present study has investigated the life-span and gene expression of both wild-type (WT) and mutants in some key components of cell signaling in response to S. typhimurium infection and protection from Lactobacillus zeae. The results indicated that the gene expression of daf-16 in the DAF/ insulin-like growth factor (DAF/IGF) pathway, ced-3 and ced-9 in the programmed cell death (PCD) pathway, lys-7, spp-1, and abf-3 for antimicrobial peptide production, and bar-1 involved in the production of other defense molecules was all significantly upregulated when the wild-type (WT) was subjected to DT104 infection. On the contrary, the gene expression of tir-1, sek-1, and pmk-1 in the p38 mitogen-activated protein kinase (MAPK) pathway and clec-60, sod-3, and skn-1 for the production of other defense molecules was significantly suppressed by DT104. Pretreatment of the worms with L. zeae LB1 significantly upregulated the expression of almost all the tested genes except for ced-3, ced-9, abf-2, age-1, and dbl-1 compared with the nematode infected with DT104 only. Mutants defective in the cell signaling or other defense molecules of C. elegans were either more susceptible (defective in nsy-1, sek-1, pmk-1, ced-3, ced-9, skn-1, or daf-16) or more resistant (defective in age-1 or dbl-1) to DT104 infection than the WT except for the mutant defective in sod-3. Mutants defective in antimicrobial peptides (lys-7 or abf-3) were also more susceptible than the WT. In contrast, the mutant defective in spp-1 became more resistant. When all the mutants were pretreated with L. zeae LB1, five mutants that are defective in nsy-1, sek-1, pmk-1, abf-3, or lys-7 showed no response to the protection from LB1. These results suggest that L. zeae LB1 can regulate C. elegans cell signaling including the p38 MAPK pathway and downstream production of antimicrobial peptides and defense molecules to combat Salmonella infection.

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“…Tan et al . (2020) explored the mechanism of inhibitory effect of P. acidilactici P25 on enterotoxigenic Escherichia coli K88, and the co‐culture results showed that the presence of P25 significantly inhibited the growth of E. coli K88. Therefore, in A3 period, Pseudomonas_sp._GR7 may be inhibited by P. acidilactici so that the abundance decreased to a very low level.…”

Section: Resultsmentioning

confidence: 99%

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

Yang

Feng

et al. 2021

Letters Applied Microbiology

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In our study, we used 16SrRNA and ITS to investigate the microbial community composition and the effect of compound bacterial agent on the microbial community composition in the aerobic composting process of food waste (FW). At the bacterial level, the main phyla of Group A (compost naturally) were Proteobacteria and Firmicutes, and the main species were Pseudomonas_sp._GR7, Bacillus licheniformis and Pediococcus acidilactici. The main phyla of Group B (compost with compound bacterial agent) were Proteobacteria, Firmicutes and Streptophyta, and the main species were Klebsiella pneumoniae, Cronobacter sakazakii, Macrococcus caseolyticus, Enterococcus faecalis, Citrobacter freundii and Bacillus velezensis. It is worth noting that M. caseolyticus may be able to improve the effect of odour which is an important sensory index during aerobic composting. At the fungal level, the main phylum of both Groups A and B was Ascomycota, and the main species of Group A were Paecilomyces variotii, Byssochlamys spectabilis and Aspergillus fumigatus. The main species of Group B were Ogataea polymorpha and Millerozyma farinosa. Finally, the degradation rate of Group B was 81% that was about 15% higher than that of Group A, indicating that the compound bacterial agent could effectively improve the degradation rate and the composting process, while the low abundance of the compound bacterial agent in the composting process might be due to the small initial addition or the inhibition of other bacteria or fungi in the composting process.

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Pediococcus acidilactici P25 Protected Caenorhabditis elegans against Enterotoxigenic Escherichia coli K88 Infection and Transcriptomic Analysis of Its Potential Mechanisms

Cited by 11 publications

References 81 publications

Probiotic Lacticaseibacillus rhamnosus GG Increased Longevity and Resistance Against Foodborne Pathogens in Caenorhabditis elegans by Regulating MicroRNA miR-34

Probiotic Lacticaseibacillus rhamnosus GG Increased Longevity and Resistance Against Foodborne Pathogens in Caenorhabditis elegans by Regulating MicroRNA miR-34

Lactobacillus Regulates Caenorhabditis elegans Cell Signaling to Combat Salmonella Infection

Effect of the compound bacterial agent on microbial community of the aerobic compost of food waste

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