<i>Pdgfr-α</i>mediates testis cord organization and fetal Leydig cell development in the XY gonad

Brennan, Jennifer; Tilmann, Christopher; Capel, Blanche

doi:10.1101/gad.1052503

Cited by 351 publications

(338 citation statements)

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“…An important role of PDGFα in controlling FLCs development was demonstrated in recent studies, showing that PDGFα-dependent signaling acts in fetal Leydig M a n u s c r i p t 18 cells (Schmahl et al, 2008) and PDGFRα null males have reduced or absent fetal Leydig cells due to attenuated proliferation of SF-1 positive precursor fetal Leydig cells (Brennan et al, 2003). Table 1 Page 27 of 34 A c c e p t e d M a n u s c r i p …”

Section: Effect Of (Bu) 2 Camp and Paracrine Factors On Pflc Prolifermentioning

confidence: 99%

“…Desert hedgehog (Dhh) and platelet-derived growth factor A (Pdfga), two Sertoli cell-derived signaling molecules, play an important role in differentiation of fetal Leydig cells (PierucciAlves et al, 2001;Yao et al, 2002;Brennan et al, 2003). Secreted by the Sertoli cell, Dhh acts in a paracrine fashion to induce the differentiation of both the fetal Leydig cells and of the peritubular myoid cells, surrounding the testicular cords (Yao et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Steroidogenesis and steroidogenic gene expression in postnatal fetal rat Leydig cells

Weisser¹,

Landreh²,

Söder³

et al. 2011

Molecular and Cellular Endocrinology

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Please cite this article as: Weisser, J., Landreh, L., Söder, O., Svechnikov, K., Steroidogenesis and steroidogenic gene expression in postnatal fetal rat Leydig cells, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2011 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Our data indicate that cAMP-activated signaling pathway(s) play an important role in the regulation of PFLCs differentiation and function.

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Section: Effect Of (Bu) 2 Camp and Paracrine Factors On Pflc Prolifermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Steroidogenesis and steroidogenic gene expression in postnatal fetal rat Leydig cells

Weisser¹,

Landreh²,

Söder³

et al. 2011

Molecular and Cellular Endocrinology

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“…For example, disruption of the GATA/FOG-2 interaction results in decreased expression of male-specific genes in the somatic cell lineages and in aberrant Leydig cell differentiation (Bielinska et al, 2007). Sertoli cell secreted factors such as desert hedgehog (Dhh) and genes expressed in Leydig cell precursors such as the Pdgf receptor-a and GATA-4 play key roles in modulating further differentiation of fetal Leydig cells (Yao et al, 2002;Brennan et al, 2003;Bielinska et al, 2007). Steroidogenicfactor 1 (SF-1), a gene expressed downstream of Sry, has a pivotal role in gonadal and adrenal organogenesis as well as being a transcriptional activator of steroid hydroxylases and other genes that are essential for androgen biosynthesis (Parker and Schimmer, 1997).…”

Section: Fetal Leydig Cellsmentioning

confidence: 99%

Key factors in the regulation of fetal and postnatal leydig cell development

Wan

Lee

2007

Journal Cellular Physiology

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The primary function of testicular Leydig cells is the production of androgens to promote sexual differentiation in the fetus, secondary sexual maturation at puberty, and spermatogenesis in the adult. The fetal and postnatal (adult) populations of Leydig cells differ morphologically and have distinct profiles of gene expression. As postnatal Leydig cells differentiate, they transition through three discrete maturational stages characterized by decreasing proliferative rate and increasing testosterone biosynthetic capacity. In this review, we discuss the development of both fetal and postnatal Leydig cells and review the regulation of this process by some of the key hormones and growth factors.

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“…At the most basic level, knockout of Sry or Sox9 leads to sex reversal through failure of Sertoli cell specification, fundamentally demonstrating the requirement of Sertoli cells for masculinization [reviewed in (Kobayashi et al ., 2005; Wilhelm et al ., 2013)]. Within the developing testis knockout of other genes can lead to absence of specific cell types, for example knockout of PDGFR‐alpha (Brennan et al ., 2003) impacts on foetal Leydig cell development, whereas knockout of desert hedgehog (DHH) (Clark et al ., 2000), or COUPTFII (Qin et al ., 2008) leads to absence of the adult Leydig cell population; W v mutations of cKit lead to near germ‐free testes, through failure of primordial germ cell migration (De Franca et al ., 1994), whereas mutations such as the cell‐specific loss of AR from Sertoli cells leads to loss of post‐meiotic germ cells (Chang et al ., 2004; De Gendt et al ., 2004). In each of these cases, however, it is challenging to assign any downstream phenotypic impact specifically to loss of gene function or to the wider effects of cell ablation.…”

Section: Approaches To Cell Ablationmentioning

confidence: 99%

Cell‐specific ablation in the testis: what have we learned?

2015

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SummaryTesticular development and function is the culmination of a complex process of autocrine, paracrine and endocrine interactions between multiple cell types. Dissecting this has classically involved the use of systemic treatments to perturb endocrine function, or more recently, transgenic models to knockout individual genes. However, targeting genes one at a time does not capture the more wide‐ranging role of each cell type in its entirety. An often overlooked, but extremely powerful approach to elucidate cellular function is the use of cell ablation strategies, specifically removing one cellular population and examining the resultant impacts on development and function. Cell ablation studies reveal a more holistic overview of cell–cell interactions. This not only identifies important roles for the ablated cell type, which warrant further downstream study, but also, and importantly, reveals functions within the tissue that occur completely independently of the ablated cell type. To date, cell ablation studies in the testis have specifically removed germ cells, Leydig cells, macrophages and recently Sertoli cells. These studies have provided great leaps in understanding not possible via other approaches; as such, cell ablation represents an essential component in the researchers’ tool‐kit, and should be viewed as a complement to the more mainstream approaches to advancing our understanding of testis biology. In this review, we summarise the cell ablation models used in the testis, and discuss what each of these have taught us about testis development and function.

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Pdgfr-αmediates testis cord organization and fetal Leydig cell development in the XY gonad

Abstract: During testis development, the rapid morphological changes initiated by

Cited by 351 publications

References 40 publications

Steroidogenesis and steroidogenic gene expression in postnatal fetal rat Leydig cells

Steroidogenesis and steroidogenic gene expression in postnatal fetal rat Leydig cells

Key factors in the regulation of fetal and postnatal leydig cell development

Cell‐specific ablation in the testis: what have we learned?

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