“…We performed combinatorial RNAi experiments using ndk, which has been successfully used to investigate the role of other genes in planarian body patterning and CNS regeneration (Felix and Aboobaker, 2010;Iglesias et al, 2011;Blassberg et al, 2013), and screened 15 genes (ascl-1, ascl-2, atoh, atoh8-1, coe, da, hesl-1, -2, -3, hlh, id4, neuroD-1, neuroD-2, sim and usf) by inspecting bHLH;ndk(RNAi) animals for changes in gpas and npp-4 expression. Induction of gpas expression posterior to the cephalic ganglia was not suppressed by inhibiting any of the bHLH genes together with ndk.…”