<i>Paraburkholderia sabiae</i>
            Uses One Type VI Secretion System (T6SS-1) as a Powerful Weapon against Notorious Plant Pathogens

Hug, Sebastian; Heiniger, Benjamin; Bolli, Kim; Paszti, Sarah; Eberl, Leo; Ahrens, Christian H.; Pessi, Gabriella

doi:10.1128/spectrum.01622-23

Cited by 2 publications

(1 citation statement)

References 84 publications

(98 reference statements)

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“…Some rhizobia can also solubilize phosphorus that is immobilized in the soil and is not available for uptake by crops 14 . Furthermore, certain strains of rhizobia can protect plants against diseases, either by stimulating the legumes’ immune system or by inhibiting growth of pathogens 17 – 19 . Among the well-established methods by which rhizobia can antagonize plants pathogens are the production of antibiotics, other toxins or the secretion of siderophores 14 .…”

Section: Introductionmentioning

confidence: 99%

RNA-seq analysis in simulated microgravity unveils down-regulation of the beta-rhizobial siderophore phymabactin

Golaz,

Papenfuhs,

Bellés-Sancho

et al. 2024

npj Microgravity

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Exploiting the symbiotic interaction between crops and nitrogen-fixing bacteria is a simple and ecological method to promote plant growth in prospective extraterrestrial human outposts. In this study, we performed an RNA-seq analysis to investigate the adaptation of the legume symbiont Paraburkholderia phymatum STM815T to simulated microgravity (s0-g) at the transcriptome level. The results revealed a drastic effect on gene expression, with roughly 23% of P. phymatum genes being differentially regulated in s0-g. Among those, 951 genes were upregulated and 858 downregulated in the cells grown in s0-g compared to terrestrial gravity (1 g). Several genes involved in posttranslational modification, protein turnover or chaperones encoding were upregulated in s0-g, while those involved in translation, ribosomal structure and biosynthesis, motility or inorganic ions transport were downregulated. Specifically, the whole phm gene cluster, previously bioinformatically predicted to be involved in the production of a hypothetical malleobactin-like siderophore, phymabactin, was 20-fold downregulated in microgravity. By constructing a mutant strain (ΔphmJK) we confirmed that the phm gene cluster codes for the only siderophore secreted by P. phymatum as assessed by the complete lack of iron chelating activity of the P. phymatum ΔphmJK mutant on chrome azurol S (CAS) agar plates. These results not only provide a deeper understanding of the physiology of symbiotic organisms exposed to space-like conditions, but also increase our knowledge of iron acquisition mechanisms in rhizobia.

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Section: Introductionmentioning

confidence: 99%

RNA-seq analysis in simulated microgravity unveils down-regulation of the beta-rhizobial siderophore phymabactin

Golaz,

Papenfuhs,

Bellés-Sancho

et al. 2024

npj Microgravity

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Identification of non‐canonical antagonistic bacteria via interspecies contact‐dependent killing

Lin,

Tao,

et al. 2024

Pest Management Science

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BackgroundCanonical biocontrol bacteria were considered to inhibit pathogenic bacteria mainly by secreting antibiotic metabolites or enzymes. Recent studies revealed that some biocontrol bacteria can inhibit pathogenic bacteria through contact‐dependent killing (CDK) mediated by contact‐dependent secretion systems. The CDK was independent of antibiotic metabolites and often ignored in normal biocontrol activity assay.ResultsIn this study, we aimed to use a pathogen enrichment strategy to isolate non‐canonical bacteria with CDK ability. Rhizosphere soil samples from Chinese cabbage showing soft rot symptom were collected and Pectobacterium carotovorum subsp. carotovorum (Pcc), the pathogen of cabbage soft rot, were added into these samples to enrich bacteria which attached on Pcc cells. By co‐culture with Pcc, four bacteria strains (named as PcE1, PcE8, PcE12 and PcE13) showed antibacterial activity were isolated from Chinese cabbage rhizosphere. These four bacteria strains showed CDK abilities to different pathogenic bacteria of horticultural plants. Among them, PcE1 was identified as Chryseobacterium cucumeris. Genome sequencing showed that PcE1 genome encoded a type VI secretion system (T6SS) gene cluster. By heterologous expression, 4 predicted T6SS effectors of PcE1 showed antibacterial activity to Escherichia coli.ConclusionOverall, this study isolated four bacteria strains with contact‐dependent killing activity to various horticultural plant pathogens, and revealed possible involvement of T6SS of C. cucumeris in antibacterial activity. These results provide valuable insight for potential application of CDK activity in biocontrol bacteria.This article is protected by copyright. All rights reserved.

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Paraburkholderia sabiae Uses One Type VI Secretion System (T6SS-1) as a Powerful Weapon against Notorious Plant Pathogens

Cited by 2 publications

References 84 publications

RNA-seq analysis in simulated microgravity unveils down-regulation of the beta-rhizobial siderophore phymabactin

RNA-seq analysis in simulated microgravity unveils down-regulation of the beta-rhizobial siderophore phymabactin

Identification of non‐canonical antagonistic bacteria via interspecies contact‐dependent killing

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