para‐AminobenzoateN‐Oxygenase

Abstract

The monooxygenase AurF catalyzes the oxidation of a primary amine during the biosynthesis of the antibiotic aureothin. Biochemical reconstitution of in vitro activity demonstrates that AurF is a nonheme iron‐dependent enzyme. Crystallographic elucidation of the 3D structure reveals homology to the ribonucleotide reductase family of monooxygenases, despite a lack of appreciable similarity in the primary sequence.