<i>PAN3</i> Encodes a Subunit of the Pab1p-Dependent Poly(A) Nuclease in <i>Saccharomyces cerevisiae</i>

Brown, Christine E.; Tarun, Salvador Z.; Boeck, Ronald; Sachs, Andalan B.

doi:10.1128/mcb.16.10.5744

Cited by 153 publications

(166 citation statements)

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“…An earlier study demonstrated that Pan2p and Pan3p, the subunits of PAN, were interacting proteins (9). We utilized the two-hybrid genetic system (3) to delineate the respective interaction domains on these two proteins.…”

Section: Mapping Of Pan2p-pan3p Interaction Domainsmentioning

confidence: 99%

“…In yeast, the relatively abundant 70-kDa poly(A)-binding protein is encoded by the PAB1 gene. Mutations in PAB1 cause a significant increase in the average steady-state poly(A) tail length of total cellular mRNA (32), and these effects have been attributed to two apparently nuclear functions of Pab1p: the regulation of a switch between processive and distributive activities in poly(A) polymerase (43) and the stimulation of PAN activity (7,9,21).…”

mentioning

confidence: 99%

“…Deletion of PAN2 and/or PAN3 eliminates poly(A) nuclease activity but does not hinder cell growth. Physical interaction between Pan2p and Pan3p has been inferred from coimmunoprecipitation and two-hybrid analyses of the full-length proteins (9).…”

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confidence: 99%

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Positive and Negative Regulation of Poly(A) Nuclease

Mangus

Evans

Agrin

et al. 2004

Molecular and Cellular Biology

102

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With rare exceptions, mRNAs whose synthesis originates within nuclei contain a 3Ј poly(A) tail. Poly(A) tracts are not encoded within genes but are added to nascent pre-mRNAs in a processing reaction that involves site-specific cleavage and subsequent polyadenylation (11,16,40,42). In Saccharomyces cerevisiae, newly synthesized poly(A) tails of different transcripts are relatively homogeneous, with their final lengths determined by the combined actions of poly(A) polymerase holoenzyme (Pap1p and Fip1p), poly(A)-binding protein (Pab1p), poly(A) nuclease (PAN), and the Pab1p-associated factor, Pbp1p (10,24,43).Poly(A) tracts are generally bound by Pab1p, a highly conserved protein with four RNA recognition motifs (RRMs) connected to a carboxy-terminal helical domain via a prolineand methionine-rich segment (25,31). Association of Pab1p with poly(A) requires a minimal binding site of 12 adenosines, and multiple molecules can bind via RRMs 1 and 2 to the same poly(A) tract, spaced approximately 25 nucleotides apart (1, 2, 31, 33). In yeast, the relatively abundant 70-kDa poly(A)-binding protein is encoded by the PAB1 gene. Mutations in PAB1 cause a significant increase in the average steady-state poly(A) tail length of total cellular mRNA (32), and these effects have been attributed to two apparently nuclear functions of Pab1p: the regulation of a switch between processive and distributive activities in poly(A) polymerase (43) and the stimulation of PAN activity (7, 9, 21).Yeast poly(A) tails are initially synthesized to default lengths of 70 to 90 A's and then trimmed to mRNA-specific lengths by PAN. Analyses of three different mRNAs indicate that such trimmed tails have lengths ranging from 55 to 71 A's (8). PAN, a Pab1p-dependent 3Ј to 5Ј poly(A) exoribonuclease, requires magnesium, releases AMP as a product, and is regulated by cis-acting mRNA sequences (21). Purified PAN contains two proteins which are essential for nuclease activity: Pan2p is a 127-kDa protein with homology to the RNase T family of 3Ј35Ј exoribonucleases, while Pan3p is a 76-kDa protein which apparently acts as a positive activator of PAN activity (8). Deletion of PAN2 and/or PAN3 eliminates poly(A) nuclease activity but does not hinder cell growth. Physical interaction between Pan2p and Pan3p has been inferred from coimmunoprecipitation and two-hybrid analyses of the full-length proteins (9).Pbp1p (Pab1p-binding protein 1) specifically interacts with a 74-amino-acid segment encompassing the proline-and methionine-rich domain of the Pab1p C terminus. PBP1 is not essential for viability, but its disruption can suppress the lethality associated with a PAB1 deletion (23). In the absence of Pbp1p, 3Ј termini of pre-mRNAs are properly cleaved but receive poly(A) tails that are, on average, 15 to 30 nucleotides shorter than normal (23,25). In vitro polyadenylation reactions using extracts from wild-type and pbp1⌬ strains demonstrated that the mutant extracts initially produced full-length tails equivalent to their wild-type counterparts but subseque...

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Section: Mapping Of Pan2p-pan3p Interaction Domainsmentioning

confidence: 99%

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confidence: 99%

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Positive and Negative Regulation of Poly(A) Nuclease

Mangus

Evans

Agrin

et al. 2004

Molecular and Cellular Biology

102

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“…ADD3 plays an important role in the skeletal organization of the cell membrane in erythrocytes, 34 ATP10A is an aminophospholipid translocase responsible for transporting amphipathic molecules, 35 and PAN3 is involved in degradation of poly(A) tails in cytoplasmic mRNA. 36 Both ADD3 and PAN3 have been reported to play a role in ALL -ADD3 is a NUP98 partner in T-ALL 37 and significantly associated with a gene expression cluster group with poor outcome in high risk BCP ALL 38 and PAN3 is recurrently deleted in high hyperdiploidy ALL. 39 Prior to this report, deletions of ATP10A have not been clearly associated with ALL.…”

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confidence: 99%

Deletions of IKZF1 and SPRED1 are associated with poor prognosis in a population-based series of pediatric B-cell precursor acute lymphoblastic leukemia diagnosed between 1992 and 2011

et al. 2013

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, Castor, A., Behrendtz, M., Biloglav, A., Forestier, E., Paulsson, K., & Johansson, B. (2014). Deletions of IKZF1 and SPRED1 are associated with poor prognosis in a population-based series of pediatric B-cell precursor acute lymphoblastic leukemia diagnosed between 1992 and 2011. Leukemia, 28(2), 302-310. DOI: 10.1038DOI: 10. /leu.2013 General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.• Users may download and print one copy of any publication from the public portal for the purpose of private study or research.• You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal and TBL1XR1 were significantly more common in B-cell precursor ALL patients who relapsed compared with those remaining in complete remission. In univariate analyses, age (≥10 years), WBC counts (>100 x 10 9 /l), t(9;22)(q34;q11), MLL rearrangements, nearhaploidy, and deletions of ATP10A, IKZF1, SPRED1, and the pseudoautosomal 1 regions on Xp/Yp were significantly associated with decreased 10-year event-free survival, with IKZF1 abnormalities being an independent risk factor in multivariate analysis irrespective of risk group. High age and deletions of IKZF1 and SPRED1 were also associated with poor overall survival. Thus, analyses of these genes provide clinically important information.

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“…5) [13,88]. Moreover, the discrepancy between NOCT in vivo and in vitro activity suggests that one or more protein partners is required for NOCT function; such a requirement has been observed previously for the PAN2 deadenylase [89,90]. Thus far, NOCT-interacting partners have not been comprehensively characterized, although one report using co-immunoprecipitation experiments in mammalian cells overexpressing tagged proteins demonstrated a physical interaction between mouse NOCT and PPARγ1 and PPARγ2 [18].…”

Section: Do Noct Protein Partners Control Its Function?mentioning

confidence: 87%

Regulatory roles of vertebrate Nocturnin: insights and remaining mysteries

2018

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Post-transcriptional control of messenger RNA (mRNA) is an important layer of gene regulation that modulates mRNA decay, translation, and localization. Eukaryotic mRNA decay begins with the catalytic removal of the 3′ poly-adenosine tail by deadenylase enzymes. Multiple deadenylases have been identified in vertebrates and are known to have distinct biological roles; among these proteins is Nocturnin, which has been linked to circadian biology, adipogenesis, osteogenesis, and obesity. Multiple studies have investigated Nocturnin’s involvement in these processes; however, a full understanding of its molecular function remains elusive. Recent studies have provided new insights by identifying putative Nocturnin-regulated mRNAs in mice and by determining the structure and regulatory activities of human Nocturnin. This review seeks to integrate these new discoveries into our understanding of Nocturnin’s regulatory functions and highlight the important remaining unanswered questions surrounding its regulation, biochemical activities, protein partners, and target mRNAs.

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PAN3 Encodes a Subunit of the Pab1p-Dependent Poly(A) Nuclease in Saccharomyces cerevisiae

Cited by 153 publications

References 49 publications

Positive and Negative Regulation of Poly(A) Nuclease

Positive and Negative Regulation of Poly(A) Nuclease

Deletions of IKZF1 and SPRED1 are associated with poor prognosis in a population-based series of pediatric B-cell precursor acute lymphoblastic leukemia diagnosed between 1992 and 2011

Regulatory roles of vertebrate Nocturnin: insights and remaining mysteries

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