Paenibacillus terrae AY-38 resistance against Botrytis cinerea in Solanum lycopersicum L. plants through defence hormones regulation

Abstract

This study aims to investigate the role of Paenibacillus terrae AY-38 to produce bioactive metabolites and to counteract pathogenic infections caused by B. cinerea. The pure culture of P. terrae (AY-38) showed the secretion of significant amount of IAA (indole-3-acetic acid) (109.57 ± 3.2 µg mL −1 ). The AY-38 strain also produced siderophore and glucanase, while in the in vitro test, it showed significant antagonism to Botrytis cinerea. In the in vivo plant experiment, the sole application of AY-38 significantly improved plant growth (plant height and leaf area), while in B. cinerea infected plants, AY-38 inoculation not only decreased the disease incidence on leaves and fruits but also reprogramed the plants for higher growth. AY-38 treatments promoted and rescued plant growth by modulating the defence responses of endogenous hormones, such as jasmonic and salicylic acids. Our findings concluded that P. terrae possesses great potential as a possible biocontrol agent against B. cinerea-induced pathogenic infections. ARTICLE HISTORY

Comparative genomic and functional analyses of Paenibacillus peoriae ZBSF16 with biocontrol potential against grapevine diseases, provide insights into its genes related to plant growth-promoting and biocontrol mechanisms

“…can produce IAA to stimulate the growth of plants, and Paenibacillus nonsymbiotic bacteria yielded high concentrations of IAA (in the range of 4.90–0.19 IAA/mg biomass; Shokri and Emtiazi, 2010 ; Trinh et al, 2018 ). P. polymyxa , P. borealis , and P. terrae showed the secretion of a significant amount of IAA, but no P. graminis had the ability to produce IAA ( Navarro-Noya et al, 2012 ; Kim et al, 2017 ). P. peoriae HJ-2 isolated from soil significantly promoted the growth of P. polyphylla , and P. peoriae ZBSF16 for the first time was used to describe the ability to synthesize IAA and promote the growth of grape, with IAA production of 28.67 μg ml −1 .…”

Ameliorative effect of indole-3-acetic acid- and siderophore-producing Leclercia adecarboxylata MO1 on cucumber plants under zinc stress

“…Siderophore production, silica and zinc solubilization L. adecarboxylata MO1 was incubated on a chrome azurol S agar (CAS) plate as previously described by Kim et al (2017) to evaluate its siderophore-producing potential. Briefly, an actively growing single colony of L. adecarboxylata MO1 was streaked onto a CAS plate and incubated at 28°C for 7 days.…”

Factors controlling the effects of mutualistic bacteria on plants associated with fungi

“…Furthermore, within a plant-fungal-bacterial mutualism experiencing a biotic stress, tomato plants associated with mycorrhizal fungi and a nematocidal bacterium gained more foliar biomass in the presence of the root knot nematode (Meloidogyne incognita) than when these tripartite symbiotic plants were grown in the absence of this nematode (Siddiqui & Sayeed Akhtar, 2009). Similarly, in a tripartite symbiosis with AF and mutualistic bacteria, tomato plants associated with a bacterium that induced host plant defences showed a greater net gain of plant height when symbiotic plants were also infected by a phytopathogen (Botrytis cinerea) compared with symbiotic plants that were pathogen-free (Kim et al, 2017).…”