p-Hydroxyacetophenone Amides from Cystobacter ferrugineus, strain Cb G35

Abstract

A family of six novel p-hydroxyacetophenone amides, 1-6, was isolated from Cystobacter ferrugineus, strain Cb G35. Their structures were elucidated by ESI-TOF mass spectrometry and NMR spectroscopy. Feeding experiments with labeled [¹³C₉,¹⁵N]-tyrosine and [d₁₀]-leucine identified the biosynthetic precursors of 1.

Isolation of Three New Diterpenes from <i>Dodonaea viscosa</i>

“…The EtOAcsoluble fraction was subjected to normal and reversed-phase silica gel column chromatography followed by repeated HPLC separation. This process gave three new compounds (1-3) as well four diterpenes [labd-13(E)-en-8,15-diol (4), 11,12) ent-15,16-epoxy-9αH-labda-13(16),14-diene-3β,8α-diol (5), 13) hautriwaic acid (6), 14,15) and 6-hydroxyhardwickjic acid (7), 16) ], four flavonoids [viscosine (8), 17) 5,7,4′-trihydroxy-3′-(4-hydroxy-3-methylbutyl)-5′-(3-methylbut-2-enyl)-3,6dimethoxyflavone (9), 10,18) alizarin (10), 18,19) and dodovisone C (11) 20) ], three coumarins [scopoletin (12), 21) isofraxidin (13), 22,23) and fraxetin (14) 24) ], one lignane [(+)-pinoresinol (15) 25) ] and five other compounds [protocatechuic acid (16), 26) (E)-cinnamic acid (17), 27) methyl hydrogen azelate (18), 28) β-hydroxylpropiovanillone (19), 29) and 4-hydroxyacetophenone (20) 30 ]. These known compounds were identified by comparing their observed spectroscopic data with previously reported data.…”

Draft Genome Sequence of Archangium sp. Strain Cb G35

“…Isolated from tree bark in Bangalore, India, Archangium sp. strain Cb G35 (DSM 52696) was reported to produce the antibiotic roimatacene, as well as six novel p -hydroxyacetophenone amides ( 4 , 5 ). Herein, we report a draft genome sequence for Archangium sp.…”

“…Archangium sp. Cb G35 has been referenced as Cystobacter ferrugineus strain Cb G35 and Cystobacter gracilis strain Cb G35 prior to a suggested reclassification ( 4 – 6 ). Genomic DNA was isolated using a GeneJET genomic DNA purification kit (Thermo Fisher).…”

Metabolomics methods for the synthetic biology of secondary metabolism

“…Several novel secondary metabolites or new isomers, as well as novel derivatives of known structures, have recently been characterized as products of cryptic biosynthetic gene clusters using this approach; many of these being promising sources of valuable molecules, e.g. bacterial regulators, antimicrobials, siderophores [40–46]. Interestingly, high‐throughput approaches for MS identification of novel compounds are now also starting to appear [47].…”