<i>OsYUC11</i>-mediated auxin biosynthesis is essential for endosperm development of rice

Xu, Xinyu; Zhiguo, E; Zhang, Dongping; Yun, Qianbin; Liang, Guohua; Niu, Baixiao; Chen, Chen

doi:10.1093/plphys/kiaa057

Cited by 64 publications

(54 citation statements)

References 79 publications

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“…This can be due to reduced cytokinin degradation due to lower transcript abundance of Cytokinin Oxidase 3 (OsCKX3) and OsCKX11 at 48 HAF (Figure 9b) (Tsai et al, 2012; Zhang et al, 2021). Recent studies highlight that auxin signalling likely regulates the early seed developmental in rice (Paul, Dhatt, Miller, et al, 2020; Xu et al, 2021). To assess the impact of heat stress on auxin accumulation, we also measured IAA and its derivatives (Table S11).…”

Section: Resultsmentioning

confidence: 99%

Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds

Sandhu

Irvin

Liu

et al. 2021

Plant Cell & Environment

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A transient heat stress occurring during early seed development in rice (Oryza sativa) reduces seed size by altering endosperm development. However, the relationship between the timing of the stress and specific developmental stage on heat sensitivity is not well-understood. To address this, we imposed a series of non-overlapping heat stress treatments and found that young seeds are most sensitive during the first two days after flowering. Temporal transcriptome analysis of developing, heat stressed (35 C) seeds during this window shows that Inositol-requiring enzyme 1 (IRE1)mediated endoplasmic reticulum (ER) stress response and jasmonic acid (JA) pathways are the early (1-3 h) drivers of heat stress response. We propose that increased JA levels under heat stress may precede ER stress response as JA application promotes the spliced form of OsbZIP50, an ER response marker gene linked to IRE1-specific pathway. This study presents temporal and mechanistic insights into the role of JA and ER stress signalling during early heat stress response of rice seeds that impact both grain size and quality. Modulating the heat sensitivity of the early sensing pathways and downstream endosperm development genes can enhance rice resilience to transient heat stress events.

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Section: Resultsmentioning

confidence: 99%

Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds

Sandhu

Irvin

Liu

et al. 2021

Plant Cell & Environment

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“…For GUS staining, the 7 DAF caryopses produced by p OsNF‐YB7:: GUS were stained at 37°C in the dark for about 8 h using a staining buffer, as previously described (Xu et al, 2020). The samples were then washed and cleared with a graded ethanol series before imaging.…”

Section: Methodsmentioning

confidence: 99%

The rice LEC1‐like transcription factor OsNF‐YB9 interacts with SPK, an endosperm‐specific sucrose synthase protein kinase, and functions in seed development

Niu

Zhang

et al. 2021

The Plant Journal

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SUMMARY LEAFY COTYLEDON1 (LEC1), a NUCLEAR FACTOR‐Y (NF‐Y) family member, plays a critical role in embryogenesis and seed development in Arabidopsis. Previous studies have shown that rice OsNF‐YB9 and OsNF‐YB7 are homologous to Arabidopsis LEC1. However, the functions of LEC1‐like genes in rice remain unclear. Here we report that OsNF‐YB9 and OsNF‐YB7 display sub‐functionalization in rice. We demonstrate that OsNF‐YB7 is expressed mainly in the embryo, whereas OsNF‐YB9 is preferentially expressed in the developing endosperm. Heterologous expression of either OsNF‐YB9 or OsNF‐YB7 in Arabidopsis lec1‐1 was able to complement the lec1‐1 defects. We failed to generate osnf‐yb7 homozygous mutants due to lethality caused by OsNF‐YB7 defects. Loss of OsNF‐YB9 function caused abnormal seed development: seeds were longer, narrower and thinner and exhibited a higher chalkiness ratio. Furthermore, the expression of genes related to starch synthesis was deregulated in osnf‐yb9. OsNF‐YB9 could interact with SPK, a sucrose synthase protein kinase that is predominantly expressed in rice endosperm. Knockout of SPK resulted in chalky seeds similar to those observed in the osnf‐yb9 mutants. Ectopic expression of OsNF‐YB9 in both rice and Arabidopsis resulted in unhealthy plants with small seeds. Taken together, these results suggest a critical role for OsNF‐YB9 in rice seed development.

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“…OsTAR1 encoding tryptophan aminotransferase, a IAA biosynthesis gene, regulate the production of IAA in the developing rice grain together with OsYUC9 and OsYUC11 (Abu-Zaitoon et al, 2012). And OsYUC11-mediated auxin biosynthesis is essential for endosperm development in rice (Xu et al, 2021b). By DNA sequencing, there was 4 SNP differences in the CDS between Xihui18 and S5.…”

Section: Discussionmentioning

confidence: 97%

QTL Analysis Based on A Rice Short-Wide Grain CSSL-Z414 and Substitution Mapping of qGL11 and qGW5

Yang

et al. 2021

Preprint

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Background: Most of rice agronomic traits as grain length etc. are complex traits controlled by multiple genes. Chromosome segment substitution lines (CSSLs) are ideal materials for dissecting and studying of these complex traits. Results: We developed a novel rice short-wide grain CSSL, Z414, deriving from progeny of the recipient parent Xihui 18 (an indica restorer line) and the donor parent Huhan 3 (a japonica cultivar). Z414 contained 4 substitution segments (average length was 3.04 Mb). Compared with Xihui 18, Z414 displayed seven significantly different traits as grain length, width and weight, chalkiness degree, brown rice rate etc. Then, 8 quantitative trait loci (QTLs) were found responding these difference traits by F2 population from Xihui 18/Z414. Among them, 6 QTLs (qPL3, qGW5, qGL11, qRLW5, qRLW11, qGWT5) could be verified by novel developed single segment substitution lines (SSSLs, S1-S6). In addition, 4 QTLs (qGL3, qGL5, qCD3 and qCD5) were novel detected by S1 and S5. Thus, the short–wide grain of Z414 was responded by qGL11, qGL3, qGL5, and qGW5. Then, qGL11 and qGW5 were delimited within intervals of 0.405 and 1.14 Mb on chromosomes 11 and 5, respectively, by substitution mapping. Again by sequencing, qRT-PCR and cell morphology analysis, qGW5 should be a novel allele of GS5 and qGL11 is novel QTL encoding CycT1;3, whose specific function of regulating grain length was still unknown. Finally, pyramid of qGL3 (a=0.22) and qGL11 (a=-0.19) displayed qGL11 epistatic to qGL3. In addition, novel S1 and D2 exhibited different grain sizes and lower chalkiness degree. They are potential to be directly used in breeding hybrid rice varieties.Conclusions: We constructed a novel rice short–wide grain CSSL-Z414 with 4 substitution segments based on the genetic backgrounds of Xihui 18. The broad grain of Z414 was controlled by qGW5, which should be a novel allele of GS5. The short grain of Z414 was controlled by qGL11, qGL3, and qGL5, and qGL11 is a novel QTL encoding CycT1;3, whose specific function of regulating grain length was still unknown, and qGL11 is epistatic to qGL3. Novel S1 and D2 are potential in hybrid rice varieties.

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OsYUC11-mediated auxin biosynthesis is essential for endosperm development of rice

Cited by 64 publications

References 79 publications

Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds

Endoplasmic reticulum stress pathway mediates the early heat stress response of developing rice seeds

The rice LEC1‐like transcription factor OsNF‐YB9 interacts with SPK, an endosperm‐specific sucrose synthase protein kinase, and functions in seed development

QTL Analysis Based on A Rice Short-Wide Grain CSSL-Z414 and Substitution Mapping of qGL11 and qGW5

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