OsSIDP366, a DUF1644 gene, positively regulates responses to drought and salt stresses in rice

Abstract

Domain of unknown function 1644 (DUF1644) is a highly conserved amino acid sequence motif present only in plants. Analysis of expression data of the family of DUF1644-containing genes indicated that they may regulate responses to abiotic stress in rice. Here we present our discovery of the role of OsSIDP366, a member of the DUF1644 gene family, in response to drought and salinity stresses in rice. Transgenic rice plants overexpressing OsSIDP366 showed enhanced drought and salinity tolerance and reduced water loss as compared to that in the control, whereas plants with downregulated OsSIDP366 expression levels using RNA interference (RNAi) were more sensitive to salinity and drought treatments. The sensitivity to abscisic acid (ABA) treatment was not changed in OsSIDP366-overexpressing plants, and OsSIDP366 expression was not affected in ABA-deficient mutants. Subcellular localization analysis revealed that OsSIDP366 is presented in the cytoplasmic foci that colocalized with protein markers for both processing bodies (PBs) and stress granules (SGs) in rice protoplasts. Digital gene expression (DGE) profile analysis indicated that stress-related genes such as SNAC1, OsHAK5 and PRs were upregulated in OsSIDP366-overexpressing plants. These results suggest that OsSIDP366 may function as a regulator of the PBs/SGs and positively regulate salt and drought resistance in rice.

Genome-Wide Analysis of the RAV Family in Soybean and Functional Identification of GmRAV-03 Involvement in Salt and Drought Stresses and Exogenous ABA Treatment

“…And water supply was withdrawn. After severe drought stress, water was added for recovery, and survival performance was photographed and recorded (Guo et al, 2016; Lu et al, 2016). At least 45 seedlings were measured for each line and each treatment.…”

Molecular characterization and function analysis of the rice OsDUF946 family

“…Some DUF-containing proteins have recently been reported to enhance the abiotic stress tolerance in plants [4][5][6][7][8].…”

A rice really interesting new gene H2‐type E3 ligase, OsSIRH2‐14, enhances salinity tolerance via ubiquitin/26S proteasome‐mediated degradation of salt‐related proteins

“…To confirm that the observed phenotypic changes in salt insensitivity were linked to changes in the expression levels of salt‐related genes, OsSNAC2 ( O. sativa stress‐responsive NAM, ATAF, and CUC; H. Hu et al, ), OsLEA3 ( O. sativa late embryogenesis abundant 3 ; T. Z. Hu, ), OsPR1b ( O. sativa pathogenesis‐related 1b ; S. Park et al, ), OsRD29A ( O. sativa responsive to dehydration 29A ; Xu et al, ), OsPBZ1 ( O. sativa ProBenzole inducible gene 1 ; C. Guo et al, ), and OsSOS1 as well as OsSIRH2‐14 interacting substrate genes ( OsHKT2;1 , OsSalT , and OsPRF2 ) were evaluated by qRT‐PCR in WT and OsSIRH2‐14 ‐overexpressing plants (Line #2; Figure ). Under the unstressed conditions, there were no clear differences in the transcript levels of the nine genes tested between WT and OsSIRH2‐14 ‐overexpressing plants (Line #2).…”

“…To confirm that the observed phenotypic changes in salt insensitivity were linked to changes in the expression levels of salt-related genes, Guo et al, 2015), and OsSOS1 as well as OsSIRH2-14 interacting substrate genes (OsHKT2;1, OsSalT, and OsPRF2) were evaluated by qRT-PCR in WT and OsSIRH2-14-overexpressing plants (Line #2; Figure 6). Under the unstressed conditions, there were no clear differences in the transcript levels of the nine genes tested between WT and OsSIRH2-14-overexpressing plants (Line #2).…”