OsHOX1 and OsHOX28 Redundantly Shape Rice Tiller Angle by Reducing HSFA2D Expression and Auxin Content

Hu, Yong; Li, Shuangle; Fan, Xin; Song, Song; Zhou, Xin; Weng, Xiaoyu; Xiao, Jinghua; Li, Xianghua; Xiong, Lizhong; You, Aiqing

doi:10.1104/pp.20.00536

Cited by 60 publications

(39 citation statements)

References 46 publications

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“…For instance, the rice root contains exodermis and sclerenchyma cell layers, which are absent from Arabidopsis. Furthermore, the multilayered cortex of the rice root can differentiate into aerenchyma, an anatomical adaptation to water submergence, at late developmental stages, a capacity that is absent in Arabidopsis 53 . The expression map of the rice root tip at single-cell resolution presented in this study provides a valuable resource for defining different cell types.…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

et al. 2021

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Root development relies on the establishment of meristematic tissues that give rise to distinct cell types that differentiate across defined temporal and spatial gradients. Dissection of the developmental trajectories and the transcriptional networks that underlie them could aid understanding of the function of the root apical meristem in both dicots and monocots. Here, we present a single-cell RNA (scRNA) sequencing and chromatin accessibility survey of rice radicles. By temporal profiling of individual root tip cells we reconstruct continuous developmental trajectories of epidermal cells and ground tissues, and elucidate regulatory networks underlying cell fate determination in these cell lineages. We further identify characteristic processes, transcriptome profiles, and marker genes for these cell types and reveal conserved and divergent root developmental pathways between dicots and monocots. Finally, we demonstrate the potential of the platform for functional genetic studies by using spatiotemporal modeling to identify a rice root meristematic mutant from a cell-specific gene cohort.

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Section: Discussionmentioning

confidence: 99%

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

et al. 2021

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“…In addition to lateral auxin transport, localized auxin biosynthesis occurring within rice shoots also regulates shoot gravitropic responses. OsHOX1 and OsHOX28 are two negative regulators that act upstream of HSFA2D and LAZY1, and inhibit the expression of multiple YUCCA genes in rice and decrease auxin biosynthesis following gravistimulation (Hu et al, 2020). Tiller Angle Control 4 (TAC4) also influences several IAA biosynthetic genes, including YUCCA and TAR2 (TRYP-TOPHAN AMINOTRANSFERASE OF ARABIDOPSIS), and regulates auxin biosynthesis and auxin distribution patterns in rice (Li et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Transcription factor OsbZIP49 controls tiller angle and plant architecture through the induction of indole‐3‐acetic acid‐amido synthetases in rice

et al. 2021

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Tiller angle is an important determinant of plant architecture in rice (Oryza sativa L.). Auxins play a critical role in determining plant architecture; however, the underlying metabolic and signaling mechanisms are still largely unknown. In this study, we have identified a member of the bZIP family of TGA class transcription factors, OsbZIP49, that participates in the regulation of plant architecture and is specifically expressed in gravity-sensing tissues, including the shoot base, nodes and lamina joints. Transgenic rice plants overexpressing OsbZIP49 displayed a tiller-spreading phenotype with reduced plant height and internode lengths. In contrast, CRISPR/Cas9-mediated knockout of OsbZIP49 resulted in a compact architecture. Follow-up studies indicated that the effects of OsbZIP49 on tiller angles are mediated through changes in shoot gravitropic responses. Additionally, we provide evidence that OsbZIP49 activates the expression of indole-3-acetic acid-amido synthetases OsGH3-2 and OsGH3-13 by directly binding to TGACG motifs located within the promoters of both genes. Increased GH3-catalyzed conjugation of indole-3-acetic acid (IAA) in rice transformants overexpressing OsbZIP49 resulted in the increased accumulation of IAA-Asp and IAA-Glu, and a reduction in local free auxin, tryptamine and IAA-Glc levels. Exogenous IAA or naphthylacetic acid (NAA) partially restored shoot gravitropic responses in OsbZIP49-overexpressing plants. Knockout of OsbZIP49 led to reduced expression of both OsGH3-2 and OsGH3-13 within the shoot base, and increased accumulation of IAA and increased OsIAA20 expression levels were observed in transformants following gravistimulation. Taken together, the present results reveal the role transcription factor OsbZIP49 plays in determining plant architecture, primarily due to its influence on local auxin homeostasis.

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“…Nipponbare cDNA using gene‐specific primers to which a Kpn I restriction site was added (Table ). The products were gel purified, cloned in the Kpn I restriction site downstream of the ubiquitin promoter in the binary vector pU1301 (Hu et al., 2020), and confirmed by DNA sequencing. The hygromycin resistance gene carried by pU1301 was used for the selection of transformed rice calli.…”

Section: Methodsmentioning

confidence: 99%

Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola‐caused giant cells in rice

Li-he

Xiao

et al. 2021

Molecular Plant Pathology

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OsHOX1 and OsHOX28 Redundantly Shape Rice Tiller Angle by Reducing HSFA2D Expression and Auxin Content

Cited by 60 publications

References 46 publications

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

Transcription factor OsbZIP49 controls tiller angle and plant architecture through the induction of indole‐3‐acetic acid‐amido synthetases in rice

Plasmodesmata play pivotal role in sucrose supply to Meloidogyne graminicola‐caused giant cells in rice

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