OsWS1 involved in cuticular wax biosynthesis is regulated by osa‐miR1848

Abstract

Cuticular wax forms a hydrophobic layer covering aerial plant organs and acting as a protective barrier against biotic and abiotic stresses. Compared with well-known wax biosynthetic pathway, molecular regulation of wax biosynthesis is less known. Here, we show that rice OsWS1, a member of the membrane-bound O-acyl transferase gene family, involved in wax biosynthesis and was regulated by an osa-miR1848. OsWS1-tagged green fluorescent protein localized to the endoplasmic reticulum (ER). Compared with wild-type rice, OsWS1 overexpression plants displayed a 3% increase in total wax, especially a 35% increase in very long-chain fatty acids, denser wax papillae around the stoma, more cuticular wax crystals formed on leaf and stem surfaces, pollen coats were thicker and more seedlings survived after water-deficit treatment. In contrast, OsWS1-RNAi and osa-miR1848 overexpression plants exhibited opposing changes. Gene expression analysis showed that overexpression of osa-miR1848 down-regulated OsWS1 transcripts; furthermore, expression profiles of OsWS1 and osa-miR1848 were inversely correlated in the leaf, panicle and stem, and upon water-deficit treatment. These results suggest that OsWS1 is regulated by osa-miR1848 and participates in cuticular wax formation.

Three endoplasmic reticulum-associated fatty acyl-coenzyme a reductases were involved in the production of primary alcohols in hexaploid wheat (Triticum aestivum L.)

“…Because of the proteins that participate in the biosynthesis of cuticular wax are mainly located in the ER in plant epidermal cells [ 17 , 49 ]. We predicted that the three TaFARs should be localized to the ER.…”

Molecular and Evolutionary Mechanisms of Cuticular Wax for Plant Drought Tolerance

“…AtACBP1 is mainly involved in the secretion of lipid components and stem cuticle formation (Xue et al, 2014). Some studies have shown that a number of proteins, which are encoded by the genes responsible for cuticular wax biosynthesis such as AtCER1 , AtCER4 , AtWAX2, At5g02890, TaFAR1, OsWS1 and CsWAX2 , were found to be localized on the ER (Kamigaki et al, 2009; Wang et al, 2015, 2016; Xia et al, 2015; Xu et al, 2017). These results showed that the ER is an important site for the synthesis of cuticular wax in plants.…”

Genome-Wide Analysis of Coding and Long Non-Coding RNAs Involved in Cuticular Wax Biosynthesis in Cabbage (Brassica oleracea L. var. capitata)

“…In recent years, several RNA transcripts with no protein coding potential have been reported that have a key role in regulation of cuticular wax biosynthesis. These noncoding RNAs include some small RNA species, such as small interfering RNA (siRNA) and microRNA (miRNA), mediated silencing transcription of the wax biosynthetic genes [18,19,20,21,22]. Cuticular wax biosynthesis also involves long noncoding RNAs (lncRNAs).…”