<i>orf137</i> triggers cytoplasmic male sterility in tomato

Kuwabara, Kosuke; Arimura, Shin‐ichi; Shirasawa, Kenta; Ariizumi, Tohru

doi:10.1093/plphys/kiac082

Cited by 23 publications

(7 citation statements)

References 7 publications

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“…The mitoTALEN-induced deletion approach is becoming the golden standard for the validation of CMS- candidate genes (Kazama et al ., 2019; Omukai et al ., 2021; Takatsuka et al ., 2022; Kuwabara et al ., 2022). Until recently, the production of male sterile plants by nuclear expression of the CMS-candidate gene fused to a mitochondrial-targeting sequence has been largely used to validate CMS-candidate genes.…”

Section: Discussionmentioning

confidence: 99%

The mitochondrialorf117Shagene desynchronizes pollen development and causes pollen abortion in the Arabidopsis Sha CMS

Dehaene,

Boussardon,

Andrey

et al. 2024

Preprint

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Cytoplasmic male sterility (CMS) is of major agronomical relevance in hybrid breeding. In gametophytic CMS, abortion of pollen is determined by the grain genotype, while in sporophytic CMS, it is determined by the mother plant genotype. While several CMS mechanisms have been dissected at the molecular level, gametophytic CMS has not been straightforwardly accessible. We used the gametophytic Sha-CMS in Arabidopsis to characterize the cause and process of pollen abortion by implementing in vivo biosensing in single pollen and mitoTALEN mutagenesis. We obtained conclusive evidence that orf117Sha is the CMS-causing gene, despite distinct characteristics from other CMS-genes. We measured the in vivo cytosolic ATP content in single pollen, followed pollen development and analyzed pollen mitochondrial volume in two genotypes that differed only by the presence of the orf117Sha locus. Our results show that the Sha-CMS is not triggered by ATP deficiency. Instead, we observed desynchronization of a pollen developmental program. Pollen death occurred independently in pollen grains at diverse stages and was preceded by mitochondrial swelling. We conclude that pollen death is grain-autonomous in Sha-CMS and propose that mitochondrial permeability transition, which was previously described as a hallmark of developmental and environmental-triggered cell death programs, precedes pollen death in Sha-CMS.

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Section: Discussionmentioning

confidence: 99%

The mitochondrialorf117Shagene desynchronizes pollen development and causes pollen abortion in the Arabidopsis Sha CMS

Dehaene,

Boussardon,

Andrey

et al. 2024

Preprint

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“…2017 ), and subsequent studies identified genes responsible for other types of CMS in rice and tomato by using mitoTALENs ( Omukai et al. 2021 , Kuwabara et al. 2022 , Takatsuka et al.…”

Section: Targeted Editing Of the Plant Mitochondrial Genomementioning

confidence: 99%

Genome Editing of Plant Mitochondrial and Chloroplast Genomes

Arimura,

Nakazato

2023

Plant and Cell Physiology

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Plastids (including chloroplasts) and mitochondria are remnants of endosymbiotic bacteria yet maintain their own genomes, which encode vital components for photosynthesis and respiration, respectively. Organellar genomes have distinctive features, such as being present as multicopies, being mostly inherited maternally, having characteristic genomic structures, and undergoing frequent homologous recombination. To date, it has proven challenging to modify these genomes. For example, while CRISPR/Cas9 is a widely used system for editing nuclear genes, it has not yet been successfully applied to organellar genomes. Recently, however, precise gene editing technologies have been successfully applied to organellar genomes. Protein-based enzymes, especially TALENs and artificial enzymes utilizing TALEs, have been successfully used to modify these genomes by harnessing organellar targeting signals. This short review introduces and discusses the use of targeted nucleases and base editors on organellar genomes, their effects, and their potential applications in plant science and breeding.

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“…So far, many mitochondria-located CMS genes have been identified in vegetable crops, e.g. ORF138 for Ogura CMS in radish ( Raphanus sativus ) [ 153 , 154 ], ORF220 in mustard ( Brassica juncea ) [ 155 , 156 ], and orf137 in tomato [ 157 ], but not all corresponding restorer genes have been identified. More information about the mechanism of CMS and its application can be found in a recent review [ 151 ].…”

Section: Perspectivesmentioning

confidence: 99%

Genic male and female sterility in vegetable crops

Cheng

Song

Zhang

2022

Horticulture Research

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Vegetable crops are greatly appreciated for their nutritional and health beneficial components. Hybrid seeds are widely used in vegetable crops for advantages such as high yield and improved resistance, which require the participation of male (stamen) and female (pistil) reproductive organs. Male- or female-sterile plants are commonly used for production of hybrid seeds or seedless fruits in vegetables. In this review, we will focus on the types of genic male sterility and factors affecting female fertility, summarize the typical gene function and research progress related to reproductive organ identity, sporophyte and gametophyte development in vegetable crops [mainly tomato (Solanum lycopersicum) and cucumber (Cucumis sativus)], and discuss the research trend and application perspectives of sterile trait in vegetable breeding and hybrid production, in order to provide reference for fertility related germplasm innovation.

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orf137 triggers cytoplasmic male sterility in tomato

Abstract: mitoTALEN, a mitochondrial genome editing technology, demonstrated that the mitochondrial gene orf137 is responsible for inducing cytoplasmic male sterility in tomato.

Cited by 23 publications

References 7 publications

The mitochondrialorf117Shagene desynchronizes pollen development and causes pollen abortion in the Arabidopsis Sha CMS

The mitochondrialorf117Shagene desynchronizes pollen development and causes pollen abortion in the Arabidopsis Sha CMS

Genome Editing of Plant Mitochondrial and Chloroplast Genomes

Genic male and female sterility in vegetable crops

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