<i>Oncopeltus fasciatus</i> as an evo‐devo research organism

Chipman, Ariel D.

doi:10.1002/dvg.23020

Cited by 30 publications

(29 citation statements)

References 68 publications

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“…Furthermore, Oncopeltus has been an established laboratory model organism for over 60 years, with a rich experimental tradition in a wide range of studies from physiology and development to evolutionary ecology [21-23]. It is among the few experimentally tractable hemimetabolous insect species, and it is amenable to a range of molecular techniques ( e.g ., [24-26]).…”

Section: Introductionmentioning

confidence: 99%

“…In fact, it was one of the first insect species to be functionally investigated by RNA interference (RNAi, [27]). RNAi in Oncopeltus is highly effective across different life history stages, which has led to a resurgence of experimental work over the past fifteen years, with a particular focus on the evolution of developmentally important regulatory genes (reviewed in [23]).…”

Section: Introductionmentioning

confidence: 99%

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Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome

Panfilio¹,

Jentzsch²,

Benoit³

et al. 2017

Preprint

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BackgroundThe Hemiptera (aphids, cicadas, and true bugs) are a key insect order, with high diversity for feeding ecology and excellent experimental tractability for molecular genetics. Building upon recent sequencing of hemipteran pests such as phloem-feeding aphids and blood-feeding bed bugs, we present the genome sequence and comparative analyses centered on the milkweed bug Oncopeltus fasciatus, a seed feeder of the family Lygaeidae.ResultsThe 926-Mb Oncopeltus genome is well represented by the current assembly and official gene set. We use our genomic and RNA-seq data not only to characterize the protein-coding gene repertoire and perform isoform-specific RNAi, but also to elucidate patterns of molecular evolution and physiology. We find ongoing, lineage-specific expansion and diversification of repressive C2H2 zinc finger proteins. The discovery of intron gain and turnover specific to the Hemiptera also prompted evaluation of lineage and genome size as predictors of gene structure evolution. Furthermore, we identify enzymatic gains and losses that correlate with feeding biology, particularly for reductions associated with derived, fluid-nutrition feeding.ConclusionsWith the milkweed bug, we now have a critical mass of sequenced species for a hemimetabolous insect order and close outgroup to the Holometabola, substantially improving the diversity of insect genomics. We thereby define commonalities among the Hemiptera and delve into how hemipteran genomes reflect distinct feeding ecologies. Given Oncopeltus's strength as an experimental model, these new sequence resources bolster the foundation for molecular research and highlight technical considerations for the analysis of medium-sized invertebrate genomes.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome

Panfilio¹,

Jentzsch²,

Benoit³

et al. 2017

Preprint

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“…Our model organism of choice is the milkweed bug Oncopeltus fasciatus (6). Oncopeltus is a member of Paraneoptera, which is the sister group to Holometabola.…”

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confidence: 99%

“…As such, it is ideally situated as an outgroup to the hyper-diverse and widely studied holometabolous insects, and can serve to polarize changes in the segmentation program in a comparison between the two most widely studied insects: Drosophila and the flour beetle Tribolium castaneum, as well as other holometabolans. Previous work on Oncopeltus has also shown that it tends to be fairly conservative, and represents many ancestral characteristics in its developmental program (6).…”

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confidence: 99%

Growth zone segmentation in the milkweed bug Oncopeltus fasciatus sheds light on the evolution of insect segmentation

Auman

Chipman

2018

Preprint

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One of the best studied developmental processes is the Drosophila segmentation cascade.However, this cascade is generally considered to be highly derived and unusual. We present a detailed analysis of the sequential segmentation cascade of the milkweed bug Oncopletus fasciatus, as a comparison to Drosophila, with the aim of reconstructing the evolution of insect segmentation. We analyzed the expression of 12 genes, representing different phases during segmentation. We reconstruct the spatio-temporal relationships among these genes And their roles and position in the cascade. We conclude that sequential segmentation in the Oncopeltus germband includes three phases: Primary pair-rule genes generate segmental gene expression in the anterior growth zone, followed by secondary pair-rule genes, expressed in the transition between the growth zone and the segmented germband. Segment polarity genes are expressed in the segmented germband. This process generates a single-segment periodicity, and does not have a double-segment pattern at any stage.Introduction:

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“…Unfortunately, these insect orders are all part of the holometabola group of hexapoda, which appeared relatively recently within the insect phylogeny ([9] and references therein). Some efforts have been made in order to fill this gap of studies in hemimetabola animal systems, such as Oncopeltus fasciatus [10], Blattella germanica [11] and water striders [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Establishment of the mayfly Cloeon dipterum as a new model system to investigate insect evolution

Almudí

Martín-Blanco

García-Fernandez

et al. 2018

Preprint

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The great capability of insects to adapt to new environments promoted their extraordinary diversification, resulting in the group of Metazoa with the largest number of species distributed worldwide. To understand this enormous diversity, it is essential to investigate lineages that would allow the reconstruction of the early events in the evolution of insects. However, research on insect ecology, physiology, development and evolution has mostly focused on few well-established model species. The key phylogenetic position of mayflies within Paleoptera, as the sister group of the rest of winged insects and life history traits of mayflies make them an essential order to understand insect evolution. Here, we describe the established of a continuous culture system of the mayfly Cloeon dipterum and a series of experimental protocols and -omics resources that allow the study of its development and its great regenerative capability. Thus, the establishment of Cloeon as an experimental platform paves the way to understand genomic and morphogenetic events that occurred at the origin of winged insects.

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Oncopeltus fasciatus as an evo‐devo research organism

Cited by 30 publications

References 68 publications

Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome

Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome

Growth zone segmentation in the milkweed bug Oncopeltus fasciatus sheds light on the evolution of insect segmentation

Establishment of the mayfly Cloeon dipterum as a new model system to investigate insect evolution

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