okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis

Ghabrial, Amin S.; Ray, Robert P.; Schüpbach, Trudi

doi:10.1101/gad.12.17.2711

Cited by 199 publications

(251 citation statements)

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“…The following mutations were used and have been previously described: spn-A 1 , spn-A 093 , spn-B BU , and spn-D 150 (Ghabrial et al 1998;Abdu et al 2003;Jang et al 2003;Staeva-Vieira et al 2003), mei-218 1 , mei-218 6 (Carpenter and Sandler 1974;McKim et al 1996), rec 1 and rec 2 (Blanton et al 2005), and lig4 57 (Gorski et al 2003;Romeijn et al 2005). Experiments with rec were done with rec 1 /rec 2 heterozygotes.…”

Section: Fly Stocks and Genetic Techniquesmentioning

confidence: 99%

“…Thus, mutation of Drosophila Rad51, spn-A, and its paralogs spn-D and spn-B, block meiotic DSB repair and accumulate DSBs into late stages of pachytene, as detected by an antibody to the phosphorylated form of H2AV (Ghabrial et al 1998;Ghabrial and Schupbach 1999;Abdu et al 2003;Jang et al 2003;Staeva-Vieira et al 2003;Mehrotra and McKim 2006). spn-D and spn-B encode orthologs of RAD51C and XRCC3, respectively, which are believed to function as a heterodimer in mammalian cells to promote DSB repair by homologous recombination (HR) (Kurumizaka et al 2001;Abdu et al 2003;Sung et al 2003;Radford and Sekelsky 2004).…”

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confidence: 99%

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Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

Joyce¹,

Paul

Chen

et al. 2012

Genetics

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Repair of meiotic double-strand breaks (DSBs) uses the homolog and recombination to yield crossovers while alternative pathways such as nonhomologous end joining (NHEJ) are suppressed. Our results indicate that NHEJ is blocked at two steps of DSB repair during meiotic prophase: first by the activity of the MCM-like protein MEI-218, which is required for crossover formation, and, second, by Rad51-related proteins SPN-B (XRCC3) and SPN-D (RAD51C), which physically interact and promote homologous recombination (HR). We further show that the MCM-like proteins also promote the activity of the DSB repair checkpoint pathway, indicating an early requirement for these proteins in DSB processing. We propose that when a meiotic DSB is formed in the absence of both MEI-218 and SPN-B or SPN-D, a DSB substrate is generated that can enter the NHEJ repair pathway. Indeed, due to its high error rate, multiple barriers may have evolved to prevent NHEJ activity during meiosis.

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Section: Fly Stocks and Genetic Techniquesmentioning

confidence: 99%

mentioning

confidence: 99%

Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

Joyce¹,

Paul

Chen

et al. 2012

Genetics

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“…5). Interestingly, several genes have been shown to be involved in the control of grk mRNA or protein localization and grk translation [aubergine (aub); cornichon (cni); encore; K10;maelstom;okra;orb;spinB;spinD;vasa;Roth et al 1995;Neuman-Silberberg and Schü pbach 1996;Wilson et al 1996;Clegg et al 1997;Hawkins et al 1997;Ghabrial et al 1998;Styhler et al 1998;Tomancak et al 1998), thus defining a pathway that is important for post-transcriptional regulation of grk and subsequent EGFR signaling. In these mutants, aspects of grk function are altered, leading to AP and DV defects reminiscent of those observed in lic.…”

Section: P38 and Erk Mapk Pathways Interact During Oogenesismentioning

confidence: 99%

“…Despite this general view and the fact that several gene products have been identified that participate in these dynamic events, the biochemical interactions that take place are still poorly understood. In addition, genetic screens have revealed that several molecularly uncharacterized genes may have a role in these processes, leading to the notion that the development of the egg chamber is more complex and requires additional gene activities and regulatory mechanisms yet to be identified (Ghabrial et al 1998).…”

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confidence: 99%

The Drosophila p38 MAPK pathway is required during oogenesis for egg asymmetric development

Irie¹,

Glise²,

Agnès³

et al. 1999

Genes & Development

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In mammalian cells, the p38 mitogen-activated protein kinase (MAPK) pathway is activated in response to a variety of environmental stresses and inflammatory stimuli. However, the role of p38 MAPK signaling in unchallenged conditions remains largely unknown. We have isolated mutations in a Drosophila p38 MAPKK gene homolog, licorne (lic), and show that during oogenesis, lic is required in the germ line for correct asymmetric development of the egg. In lic mutant egg chambers, oskar mRNA posterior localization is not properly maintained, resulting in anteroposterior patterning defects in the embryo. Furthermore, lic loss-of-function in the germ line leads to reduced EGF receptor activity in dorsal follicle cells and ventralization of the egg shell. Both these defects are associated with a diminution of gurken protein levels in the oocyte. Our phenotypic data argue for a role of lic in a post-transcriptional regulation of the grk gene. Furthermore, they show that in addition to the well-characterized Ras/Raf/ERK MAPK pathway acting in the follicle cells, another related signaling cascade, the p38 MAPK pathway, is required in the germ line for correct axes determination. These results provide the first genetic demonstration of an essential function for a p38 pathway during development.

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“…RAD51B, RAD51D, and XRCC2 knockout mutant mice died during embryonic development (Shu et al, 1999;Deans et al, 2000;Pittman and Schimenti, 2000). The RAD51C and XRCC3 mutants in Drosophila (Spn-D and Spn-B, respectively) were partially sterile, defective for meiotic recombination, but not hypersensitive to DSB-inducing agents (Ghabrial et al, 1998;Abdu et al, 2003).…”

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confidence: 99%

Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

et al. 2005

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Rad51 is a homolog of the bacterial RecA recombinase, and a key factor in homologous recombination in eukaryotes. Rad51 paralogs have been identified from yeast to vertebrates. Rad51 paralogs are thought to play an important role in the assembly or stabilization of Rad51 that promotes homologous pairing and strand exchange reactions. We previously characterized two RAD51 paralogous genes in Arabidopsis (Arabidopsis thaliana) named AtRAD51C and AtXRCC3, which are homologs of human RAD51C and XRCC3, respectively, and described the interaction of their products in a yeast two-hybrid system. Recent studies showed the involvement of AtXrcc3 in DNA repair and functional role in meiosis. To determine the role of RAD51C in meiotic and mitotic recombination in higher plants, we characterized a T-DNA insertion mutant of AtRAD51C. Although the atrad51C mutant grew normally during vegetative developmental stage, the mutant produced aborted siliques, and their anthers did not contain mature pollen grains. Crossing of the mutant with wild-type plants showed defective male and female gametogeneses as evidenced by lack of seed production. Furthermore, meiosis was severely disturbed in the mutant. The atrad51C mutant also showed increased sensitivity to g-irradiation and cisplatin, which are known to induce double-strand DNA breaks. The efficiency of homologous recombination in somatic cells in the mutant was markedly reduced relative to that in wild-type plants.

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okra and spindle-B encode components of the RAD52 DNA repair pathway and affect meiosis and patterning in Drosophila oogenesis

Cited by 199 publications

References 45 publications

Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

Multiple Barriers to Nonhomologous DNA End Joining During Meiosis inDrosophila

The Drosophila p38 MAPK pathway is required during oogenesis for egg asymmetric development

Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

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