<i>Ocimum gratissimum</i>retards breast cancer growth and progression and is a natural inhibitor of matrix metalloproteases

Nangia‐Makker, Pratima; Raz, Tirza; Tait, Larry; Shekhar, Malathy P.V.; Li, Hong; Balan, Vitaly; Makker, Hemanckur; Fridman, Rafael; Maddipati, Krishna Rao; Raz, Avraham

doi:10.4161/cbt.23762

Cited by 37 publications

(16 citation statements)

References 44 publications

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“…MCF10A cells were kindly provided by Dr. Yosef Yarden (Weizmann Institute of Science, Rehovot, Israel) in December 2009 and were cultured as described (19). MCF10AT1 cells were kindly provided by Dr. Avraham Raz (Wayne State University, Detroit, MI) (20) in January 2010. Cells were infected with control empty vector (Mock), heparanase or its C-terminal domain (8C) (17) gene constructs, selected with Blasticidin (10μg/ml; Invitrogen), expended and pooled.…”

Section: Methodsmentioning

confidence: 99%

Heparanase Cooperates with Ras to Drive Breast and Skin Tumorigenesis

Boyango¹,

Barash²,

Naroditsky

et al. 2014

Cancer Research

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Heparanase has been implicated in cancer but its contribution to the early stages of cancer development is uncertain. In this study, we utilized non-transformed human MCF10A mammary epithelial cells and two genetic mouse models (Hpa-transgenic and knockout mice) to explore heparanase function at early stages of tumor development. Heparanase overexpression resulted in significantly enlarged asymmetrical acinar structures, indicating increased cell proliferation and decreased organization. This phenotype was enhanced by co-expression of heparanase variants with a mutant H-Ras gene, which was sufficient to enable growth of invasive carcinoma in vivo. These observations were extended in vivo by comparing the response of Hpa-transgenic (Hpa-Tg) mice to a classical two-stage DMBA/TPA protocol for skin carcinogenesis. Hpa-Tg mice overexpressing heparanase were far more sensitive than control mice to DMBA/TPA treatment, exhibiting a 10-fold increase in the number and size of tumor lesions. Conversely, DMBA/TPA-induced tumor formation was greatly attenuated in Hpa-KO mice lacking heparanase, pointing to a critical role of heparanase in skin tumorigenesis. In support of these observations, the heparanase inhibitor PG545 potently suppressed tumor progression in this model system. Taken together, our findings establish that heparanase exerts pro-tumorigenic properties at early stages of tumor initiation, co-operating with Ras to dramatically promote malignant development.

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Section: Methodsmentioning

confidence: 99%

Heparanase Cooperates with Ras to Drive Breast and Skin Tumorigenesis

Boyango¹,

Barash²,

Naroditsky

et al. 2014

Cancer Research

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“…[10] OS is a dietary herb and well known for its multiple beneficial pharmacologic properties including anti-cancer activity. [17] Plant possesses antineoplastic effects and it can be used for the prevention and treatment of human cancer. Crude extract of OG and its hydrophobic and hydrophilic fractions (HB and HL) differentially inhibit breast cancer cell chemotaxis and chemoinvasion in vitro and retard tumor growth and temporal progression of MCF10ADCIS.com xenografts, a model of human breast comedo-ductal carcinoma in situ (comedo-DCIS).…”

Section: Anticancer Activitymentioning

confidence: 99%

“…OG is non-toxic and obstruct cancerous activity MMP inhibitory activity [ Table 1]. [17] Tulsi (OS Linn) extract shows apoptosis-inducing ability on LNCaP prostate cancer cells. [5] When LNCaP prostate cancer cells were treated with different concentrations of 70% ethanolic extract of Tulsi (EET), they show cytotoxicity after 24 and 48h of treatment.…”

Section: Anticancer Activitymentioning

confidence: 99%

Untitled

Upadhyay

2017

IJGP

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The present review article explains medicinal and therapeutic uses of Tulsi plant in traditional medicine. Tulsi (Ocimum sanctum [OS] Linn.) is an aromatic plant belongs to family Lamiaceae. It is traditionally used for preparation of various Ayurvedic formulations for treatment of bronchitis, influenza, and asthma. Hot concoction of Tulsi leaves is usually provided for immediate relief in cold, sneezing nose, cough, malaria, and dengue. This article explains the biological effectiveness of OS against diabetes mellitus, hypertension, cancers, respiratory diseases, arthritis, various microbes, and parasites. Tulsi extracts and its various bio-organic constituents showed antioxidant activity, anti-atherogenic effect, anti-aging, immunomodulatory, anti-inflammatory, antistress, hepatoprotective, radioprotective, antihelmintic, repellent and larvicidal activity. Tulsi active ingredients showed anti-inflammatory properties and also play a role in modulation of both cellular and humeral immunity. Plant shows healing properties in hepatic injury and gastric ulcer. It relieves from stress, restore and improve body immunity and digestion. Ocimum basilicum L. contains (-)-linalool, eugenol and methyl chavicol, methyl chavicol (93.0%), gamma-caryophyllene as major constituents. Minor oil constituents are (+)-delta-cadinene, 3-carene, alpha-humulene, citral, and (-)-trans-caryophyllene. Tulsi oil contains high alpha-linolenic acid contents mainly eicosanoid precursor polyunsaturated fatty acids which are anti-inflammatory in nature. Tulsi also contains camphor, caryophyllene oxide, cineole, methyleugenol, limonene, myrcene, and thymol, which are known insect repellents. Its essential oil (EO) can be used to abate the growth of mosquitoes and control malaria. It is a good repellant and can be used for deterring flies, mosquitoes and insects. No doubt Tulsi plant is a good source of natural products mainly phyto-constituents and EO which can be used as alternative medicine for the treatment of various ailments and human health problems but proper composition and appropriate formulation is required before being used.

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“…A concentration-dependent anti-proliferative activity of OG was documented in prostate cancer (PC-3) cells in-vitro (Ekunwe et al ., 2010). Aqueous leaf extract of OG also inhibited proliferation, migration, anchorage independent growth, morphogenesis, induction of COX-2 protein (Nangia-Makker et al ., 2007) and matrix metaloproteases (Nangia-Makker et al ., 2013) in breast cancer cells. Such tumor prevention potential is common in plants with polyphenolic compounds, anti-oxidants, vitamins, and v-3 fatty acids (Fahey et al ., 1997; Pezzuto, 1997; Kobayashi et al ., 2000); it is therefore not surprising that polyphenolic and antioxidant components were reported in OG (Venuprasad et al ., 2014).…”

Section: Discussionmentioning

confidence: 99%

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

Shittu

Olatunji

et al. 2019

JBRA Assisted Reproduction

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Objective:This study was designed to investigate the Ocimum gratissimum (OG) effects on sperm quality and testicular cytoarchitecture in alloxan-induced diabetic rats.Method:Twenty male Wistar rats (150-200 g) were assigned into 4 groups (n=5) as A (control), B (OG), C (Dia) and D (Dia+OG). Groups A and B were normal animals receiving distilled water or OG (400 mg/kg), respectively while diabetes was induced by alloxan monohydrate (100 mg/kg) in groups C and D, followed by the administration of distilled water or OG, respectively for 28 days. Blood samples were obtained for fasting blood glucose (FBG) and fructosamine determination while, epididymis and testes were obtained for sperm quality assessment using computer-assisted sperm analyzer and testicular histomorphometry, respectively. Seminiferous tubule diameter and interstitial space distance were quantified in hematoxylin and eosin stained slides. Statistical analysis was done using ANOVA and student t-test at α0.05.Results:Fructosamine and FBG were reduced in Dia+OG (80.11±3.80µmol/L and 132.0±8.41mg/dl, respectively) compared with Dia (139.66±4.29µmol/L and 285.6±26.69mg/dl, respectively). Sperm count was unchanged in Dia, but decreased in OG and Dia+OG; abnormal sperm cells increased in OG, Dia and Dia+OG. Mild vacuolation in the seminiferous tubule, disorganized germinal cells layer, arrested sperm maturation with empty spermatozoa in lumen, decreased seminiferous tubule diameter and increased interstitial space were found in the testes of OG, Dia and Dia+OG compared with control.Conclusion:Diabetes induces sperm impairments and distortions in testicular cytoarchitecture, which were aggravated by OG leaf extract in male Wistar rats.

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Ocimum gratissimumretards breast cancer growth and progression and is a natural inhibitor of matrix metalloproteases

Cited by 37 publications

References 44 publications

Heparanase Cooperates with Ras to Drive Breast and Skin Tumorigenesis

Heparanase Cooperates with Ras to Drive Breast and Skin Tumorigenesis

Untitled

Ocimum gratissimum leaf extract may precipitate infertility in male diabetic Wistar rats

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