<i>o</i>-PHTHALALDEHYDE (OPT) A SENSITIVE DETECTION REAGENT FOR GLUCAGON, SECRETIN AND VASOACTIVE INTESTINAL PEPTIDE

Håkanson, R.; Owman, Christer; Sundler, F.

doi:10.1177/20.2.138

Cited by 8 publications

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“…The peptide hormones glucagon and secretin showed strong fluorescence after reaction with o-phthalaldehyde (316,318); the same reagent was used for visualization and determination of proteins in gel electrophoresis (975). A sensitive fluorescence reaction for amino acids is condensation with pyridoxal, followed by either reduction with borohydride (553) or chelation with zinc ion (558); the latter was adapted to the automated assay of amino acids in protein hydrolyzates (560).…”

Section: Organic and Biologicalmentioning

confidence: 99%

Fluorometric analysis

Weissler¹

1974

Anal. Chem.

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This review is dedicated to the memory of Prof. Charles E. White, who was at first the sole author and then the senior author of the 13 previous biennial reviews in this series. He was a leader in establishing the value and importance of fluorescence analysis; he was also a good and warm-hearted person, whose death in May 1973 was a real loss.were found to be very sensitive fluorescence reagents for detection of Zn, Al, Sc, Ga, and In, as well as suitable for quantitation of, for example, Zn at pH 6.9-7.2 in 80% EtOH (845). Acridine fluorescence quenching by iodine was proposed for the indirect determination of Au, Cr, Se,

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Section: Organic and Biologicalmentioning

confidence: 99%

Fluorometric analysis

Weissler¹

1974

Anal. Chem.

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Ontogeny of histamine-immunoreactive cells in rat stomach

1992

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An antiserum against hemocyanin-conjugated histamine was used to study the cellular stores of histamine in the stomach, especially the oxyntic mucosa, of fetal and early postnatal rats. Tissues were fixed in 4% 1-ethyl-3(3-dimethyl-aminopropyl) carbodiimide (EDC-DI) and standard immunofluorescence technique was used. Histamine was first detected on the 16th embryonic (E16) day when a few histamine-immunoreactive (HA-ir) cells and nerve fibers were observed in the muscular layer of the stomach wall. On day E18, HA-ir cells were visualized for the first time in the oxyntic mucosa of the stomach, and from that day on the number of such cells increased slowly initially and after day E20 more rapidly. At birth many of the HA-ir cells in the oxyntic mucosa possessed processes giving them a paracrine-like appearance typical of enterochromaffin-like cells (ECL cells). Only a very small number of the HA-ir cells represented metachromatically stained mast cells and were located in the submucosa. After birth, the number of HA-ir ECL cells increased steadily, until day 21 when the distribution and number was very similar to that of the adult. The results suggest that histamine-containing neurons and ECL cells appear in the stomach wall before birth, and that there are histamine-containing ECL cells in the mucosa and mast cells in the submucosa of the stomach wall at birth.

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