<i>Number 1</i> Epithelial biology

Scully, Crispian; Jv, Bagan; Black, Martin M.; Carrozzo, M; Eisen, Damon P.; Escudier, Michael; Farthing, Paula M.; Küffer, R; Muzio, Lorenzo Lo; Mignogna, Michele Davide; Porter, Stephen

doi:10.1111/j.1601-0825.2004.01078.x

Cited by 16 publications

(12 citation statements)

References 169 publications

(196 reference statements)

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“…Among various factors that played a role in cell attachment and speading were cohesion among keratinocytes and adhesion of keratinocytes to BC film. There were several types of junctional structures such as desmosomes and adherens junctions, which consisted of several proteins crucial for intercellular adhesion of oral and skin keratinocytes (22). E‐Cadherin was one of several adhesion molecules and was usually used as a characteristic marker for normal differentiation stage of keratinocytes (23–25).…”

Section: Resultsmentioning

confidence: 99%

“…It was reported that laminin isoforms with the α‐3 chain such as laminin 5 were primarily required to maintain differentiation stage, stable anchorage, and spreading of keratinocytes in adult skin. The fact that the lack of laminin α‐3 caused skin blistering suggested the important role of this molecule in keratinocyte homeostasis (22, 29, 30).…”

Section: Resultsmentioning

confidence: 99%

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Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film

et al. 2006

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Thin films of bacterial cellulose (BC) from a nata de coco culture system were developed, characterized, and investigated for the growth of human keratinocytes and fibroblasts. The average pore diameter and total surface area of the dried BC films estimated by BET were 224 A and 12.62 m(2)/g, respectively. With an film thickness of 0.12 mm, the average tensile strength and break strain of the dried films were 5.21 MPa and 3.75%, whereas those of the wet films were 1.56 MPa and 8.00%, respectively. The water absorption capacity of air-dried film was 5.09 g water/g dried films. For uses in the therapy of skin wounds, the potential biological mechanism of action of BC film was evaluated by using human keratinocytes and fibroblasts. Our results were the first direct demonstration that BC film supported the growth, spreading, and migration of human keratinocytes but not those of human fibroblasts. Expressions of E-cadherin and the alpha-3 chain of laminin confirmed the phenotype of human keratinocytes on BC film.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film

et al. 2006

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“…Invasiveness reflects the ability of the bacteria to adhere to host cells and penetrate through the structural barriers including the epithelium and extracellular matrix. Periodontal tissue is primarily composed of type I collagen, which is made up of three parallel polypeptide chains of the sequence Gly‐X‐Y, with X representing proline and Y representing hydroxyproline (Scully et al ., ). These amino acids give collagen stability and restrict the rotation of the polypeptide backbone (Dung & Liu, ).…”

Section: Discussionmentioning

confidence: 97%

“…It is the major component of the gingival connective tissue, connecting the cementum and alveolar bone (Nanci & Bosshardt, ). Periodontal tissues such as dentine, cementum and pulp are made up of mostly type 1 collagen (Scully et al ., ). Therefore an investigation into the mechanisms employed by periodontopathogenic bacteria to compromise the physical barrier of the host's collagen‐rich gingival ECM is relevant to understand its virulence and pathogenicity.…”

Section: Introductionmentioning

confidence: 97%

Filifactor alocis collagenase can modulate apoptosis of normal oral keratinocytes

Chioma

Aruni

Milford

et al. 2016

Molecular Oral Microbiology

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SUMMARY To successfully colonize host cells, pathogenic bacteria must circumvent the host’s structural barrier such as the collagen-rich extracellular matrix (ECM), as a preliminary step to invasion and colonization of the periodontal tissue. Filifactor alocis possesses a putative Peptidase U32 family protein (HMPREF0389_00504) with collagenase activity that may play a significant role in colonization of host tissue during periodontitis by breaking down collagen into peptides and disruption of the host cell. Domain architecture the HMPREF0389_00504 protein predicted the presence of a characteristic PrtC-like collagenase domain, and a peptidase domain. Our study demonstrated that the recombinant F. alocis peptidase U32 protein (designated PrtFAC) can interact with, and degrade type I collagen, heat denatured collagen and gelatin in a calcium dependent manner. PrtFAC decreased viability and induced apoptosis of normal oral keratinocytes (NOKs) in a time and dose dependent manner. Transcriptome analysis of NOK cells treated with PrtFAC showed an upregulation of the genes encoding human pro-apoptotic proteins: Apoptotic Peptidase Activating Factor 1 (Apaf1) Cytochrome C, as well as Caspase 3 and 9, suggesting the involvement of the mitochondrial apoptotic pathway. There was a significant increase in caspase 3/7 activity in NOK cells treated with PrtFAC. Taken together, these findings suggest that F. alocis PrtFAC protein may play a role in the virulence and pathogenesis of F. alocis.

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“…Não há estudos na população brasileira referentes a genes que conferem susceptibilidade ao Pênfigo Vulgar. desmossomos, e à membrana basal pelos hemidesmossomos (52) (figura 1). (53) ).…”

Section: Introductionunclassified

Estudo da associação entre antígenos de histocompatibilidade leucocitária (HLA) e pênfigo vulgar em pacientes brasileiros

Weber¹

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Number 1 Epithelial biology

Abstract: The oral mucous membrane has features similar to skin but also differs in several ways. This paper reviews the aspects of epithelial biology necessary for an understanding of the vesiculoerosive disorders.

Cited by 16 publications

References 169 publications

Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film

Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film

Filifactor alocis collagenase can modulate apoptosis of normal oral keratinocytes

Estudo da associação entre antígenos de histocompatibilidade leucocitária (HLA) e pênfigo vulgar em pacientes brasileiros

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