<i>NTS</i> Targeted By miR-182 Modulates the Apoptosis of Goat Endometrial Epithelial Cells

An, Xiaopeng; Zhang, Yue; Liu, Jidan; Ma, Xin; Guo, Zhaoyu; Cao, Binyun

doi:10.2478/acve-2019-0028

Cited by 4 publications

(1 citation statement)

References 13 publications

(14 reference statements)

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“…Recently, researchers found that miRNAs can bind to 3’ untranslated region (3’ UTR) of target messenger RNAs (mRNAs) and negatively regulate gene expression by transcriptional or translational inhibition [ 1 , 2 , 3 ]. MiR-574-5p are implicated in the pathophysiology of many tumors, including breast cancer, colorectal cancer and other oncoma [ 4 , 5 , 6 ]. In addition, miR-574-5p is a very important regulator for cell migration and proliferation [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

Zhang

Liu

et al. 2020

Genes

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The development of the udder and the milk yield are closely related to the number and vitality of mammary epithelial cells. Many previous studies have proved that non-coding RNAs (ncRNAs) are widely involved in mammary gland development and the physiological activities of lactation. Our laboratory previous sequencing data revealed that miR-574-5p was differentially expressed during the colostrum and peak lactation stages, while the molecular mechanism of the regulatory effect of miR-574-5p on goat mammary epithelial cells (GMECs) is unclear. In this study, the targeting relationship was detected between miR-574-5p or ecotropic viral integration site 5-like (EVI5L) and circRNA-006258. The results declared that miR-574-5p induced the down-regulation of EVI5L expression at both the mRNA and protein levels, while circRNA-006258 relieved the inhibitory effect through adsorbing miR-574-5p. EVI5L blocked the G1 phase and promoted the S phase by activating the Rab23/ITGB1/TIAM1/Rac1-TGF-β/Smad pathway in GMECs. By increasing the protein expression of Bcl2 and reducing the protein expression of Bax, EVI5L promoted cell growth and inhibited apoptosis. The activation of the PI3K/AKT–mTOR signaling pathway promoted the production of triacylglycerol (TAG) and β-casein in GMECs. The circRNA–006258/miR-574-5p/EVI5L axis could regulate the cell growth and milk synthesis of GMECs by sponge-adsorbed miR-574-5p. These results would provide scientific evidence for precision animal breeding in the industry of dairy goats.

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Section: Introductionmentioning

confidence: 99%

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

Zhang

Liu

et al. 2020

Genes

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Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland

Zhang

Liu

Niu

et al. 2023

Developmental Biology

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Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland

Zhang

Liu²,

Niu

et al. 2022

Preprint

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A healthy mammary gland is a necessity for milk production of dairy goats. The role of chi-miR-3880 in goat lactation is illustrated in our previous study. Among the differentially expressed genes regulated by chi-miR-3880, one seventh are interferon stimulated genes, including MX1, MX2, IFIT3, IFI44L, and DDX58. As the inflammatory cytokine interferon gamma (IFNγ) has been identified as a potential marker of caseous lymphadenitis in lactating sheep, the interaction between IFNγ and immune-related microRNAs is explored. In this study, it was found that chi-miR-3880 could be one of the microRNAs downregulated by IFNγ in goat mammary epithelial cells (GMECs). The regulation among IFNγ, chi-miR-3880, and DDX58, as well as their roles in mammary gland were investigated. The study illustrated that IFNγ/chi-miR-3880/DDX58 axis modulates GMEC proliferation and lipid formation through PI3K/AKT/mTOR pathway, and regulates apoptosis through Caspase-3 and Bcl-2/Bax. The role of the axis in involution was reflected by the expression of p53 and NF-κB. In conclusion, IFNγ/chi-miR-3880/DDX58 axis plays an important part in lactation.

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NTS Targeted By miR-182 Modulates the Apoptosis of Goat Endometrial Epithelial Cells

Cited by 4 publications

References 13 publications

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

CircRNA-006258 Sponge-Adsorbs miR-574-5p to Regulate Cell Growth and Milk Synthesis via EVI5L in Goat Mammary Epithelial Cells

Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland

Chi-miR-3880 mediates the regulatory role of interferon gamma in goat mammary gland

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