<i>NosZ</i> gene cloning, reduction performance and structure of <i>Pseudomonas citronellolis</i> WXP-4 nitrous oxide reductase

Hu, Liyong; Wang, Xiaoping; Chen, Cong; Chen, Jianmeng; Wang, Zeyu; Hrynshpan, Dzmitry; Savitskaya, Tatsiana

doi:10.1039/d1ra09008a

Cited by 6 publications

(4 citation statements)

References 44 publications

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“…2) is the only known enzyme responsible for reducing nitrous oxide emissions. 15 By metagenomics and metatranscriptomics, the activity of microorganisms expressing NosZ can be estimated at hotspots of GHG emissions through the identification of microorganisms encoding the NosZ gene and their mRNA transcripts. This information can be used to identify optimal operational conditions (such as carbon and nitrogen load, pH, dissolved oxygen level, and temperature) at which N 2 OR activities are the highest in real time.…”

Section: Real-time Genomics To Guiding Treatment Operationsmentioning

confidence: 99%

Mitigating greenhouse gas emissions from waste treatment through microbiological innovation

2023

Microbiol. Aust.

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The emission of greenhouse gases (GHGs) from the treatment of municipal, agricultural and industrial waste occurs in virtually every city on our planet. This is due to various microbial activities at different stages of waste treatment. Traditional treatment methods have a significant environmental impact, producing methane, carbon dioxide and nitrous oxide emissions, in addition to demanding high energy input and having low treatment efficiencies. To address these issues, the Australian water and waste sectors are shifting towards the adoption of next-generation, carbon-neutral treatment options. Here I discuss our current knowledge gaps in mitigating GHG emissions from waste streams, with a focus on wastewater treatment plants. I highlight the application of real-time genomics to identify sources of GHG emissions, monitor mitigation efforts, assist process operation and guide plant operations. I also emphasise recent innovations of microbial processes that capture GHG from waste and upgrade them into higher value products. Ultimately, combined effort across disciplines is required to proactively mitigate the global threat of climate change.

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Section: Real-time Genomics To Guiding Treatment Operationsmentioning

confidence: 99%

Mitigating greenhouse gas emissions from waste treatment through microbiological innovation

2023

Microbiol. Aust.

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“…Figure 6 shows the dynamics of 12 genera involved in N 2 O reduction during the rhizoremediation of diesel-contaminated soil. Bacterial species belonging to Bradyrhizobium, Aminobacter, Mesorhizobium, Shinella, Paracoccus, Azospirillum, Alcaligenes, Castellaniella, and Pseudomonas have been reported to reduce N 2 O [49][50][51][52][53][54][55][56][57][58][59][60][61] . Some also contain nosZ genes, which are responsible for the reduction of N 2 O to N 2 52,54,57,59,[62][63][64][65] .…”

Section: Sampling Time (D)mentioning

confidence: 99%

Inoculation effect of Pseudomonas sp. TF716 on N2O emissions during rhizoremediation of diesel-contaminated soil

Kim

Cho

2022

Sci Rep

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The demand for rhizoremediation technology that can minimize greenhouse gas emissions while effectively removing pollutants in order to mitigate climate change has increased. The inoculation effect of N2O-reducing Pseudomonas sp. TF716 on N2O emissions and on remediation performance during the rhizoremediation of diesel-contaminated soil planted with tall fescue (Festuca arundinacea) or maize (Zea mays) was investigated. Pseudomonas sp. TF716 was isolated from the rhizosphere soil of tall fescue. The maximum N2O reduction rate of TF716 was 18.9 mmol N2O g dry cells−1 h−1, which is superior to the rates for previously reported Pseudomonas spp. When Pseudomonas sp. TF716 was added to diesel-contaminated soil planted with tall fescue, the soil N2O-reduction potential was 2.88 times higher than that of soil with no inoculation during the initial period (0–19 d), and 1.08–1.13 times higher thereafter. However, there was no enhancement in the N2O-reduction potential for the soil planted with maize following inoculation with strain TF716. In addition, TF716 inoculation did not significantly affect diesel degradation during rhizoremediation, suggesting that the activity of those microorganisms involved in diesel degradation was unaffected by TF716 treatment. Analysis of the dynamics of the bacterial genera associated with N2O reduction showed that Pseudomonas had the highest relative abundance during the rhizoremediation of diesel-contaminated soil planted with tall fescue and treated with strain TF716. Overall, these results suggest that N2O emissions during the rhizoremediation of diesel-contaminated soil using tall fescue can be reduced with the addition of Pseudomonas sp. TF716.

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“…NorB and NorC catalyze the reduction of NO to N 2 O, with NorB transferring electrons to the active site of NorC to facilitate the reaction[63]. Finally, the NosZ enzyme catalyzes the reduction of N 2 O to N 2[64,65].active in aerobic environments[62]. NirS and NirK are key enzymes that catalyze the reduction of NO2 − to the gaseous compound NO.…”

mentioning

confidence: 99%

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confidence: 99%

Impact of Nitrate on the Removal of Pollutants from Water in Reducing Gas-Based Membrane Biofilm Reactors: A Review

Zhang,

Huang,

et al. 2024

Membranes

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The membrane biofilm reactor (MBfR) is a novel wastewater treatment technology, garnering attention due to its high gas utilization rate and effective pollutant removal capability. This paper outlines the working mechanism, advantages, and disadvantages of MBfR, and the denitrification pathways, assessing the efficacy of MBfR in removing oxidized pollutants (sulfate (SO4−), perchlorate (ClO4−)), heavy metal ions (chromates (Cr(VI)), selenates (Se(VI))), and organic pollutants (tetracycline (TC), p-chloronitrobenzene (p-CNB)), and delves into the role of related microorganisms. Specifically, through the addition of nitrates (NO3−), this paper analyzes its impact on the removal efficiency of other pollutants and explores the changes in microbial communities. The results of the study show that NO3− inhibits the removal of other pollutants (oxidizing pollutants, heavy metal ions and organic pollutants), etc., in the simultaneous removal of multiple pollutants by MBfR.

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NosZ gene cloning, reduction performance and structure of Pseudomonas citronellolis WXP-4 nitrous oxide reductase

Abstract: Nitrous oxide reductase (N2OR) is the only known enzyme that can reduce the powerful greenhouse gas nitrous oxide (N2O) to harmless nitrogen at the final step of bacterial denitrification. The recombinant E. coli and wild strain WXP-4 demonstrate strong N2O reduction ability.

Cited by 6 publications

References 44 publications

Mitigating greenhouse gas emissions from waste treatment through microbiological innovation

Mitigating greenhouse gas emissions from waste treatment through microbiological innovation

Inoculation effect of Pseudomonas sp. TF716 on N2O emissions during rhizoremediation of diesel-contaminated soil

Impact of Nitrate on the Removal of Pollutants from Water in Reducing Gas-Based Membrane Biofilm Reactors: A Review

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