<i>Nocardia</i> fractions, NLD and NWSM, induce tumor necrosis factor‐α secretion in human monocytes: Role of protein kinase C

Jl, Mege; Mv, Sanguedolce; Jacob, Timothy D.; Bongrand, Pierre; Capo, Christian; Eb, Myssiakine; Barot‐Ciorbaru, R.

doi:10.1002/eji.1830230728

Cited by 14 publications

(6 citation statements)

References 33 publications

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“…Next, 10 5 PBMC ml 21 were incubated for 1 h at 37 uC in 24-well cell-culture plates (Corning). Nonadherent cells were removed by washing and the remaining adherent cells were considered to be monocytes, since more than 95 % of these cells expressed CD14 (Capo et al, 1996;Mege et al, 1993). Monocytes were further differentiated into macrophages by incubation at 37 uC for 7 days in the presence of fetal calf serum.…”

Section: Methodsmentioning

confidence: 99%

Early intracellular trafficking of Waddlia chondrophila in human macrophages

Croxatto

Greub

2010

Microbiology

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Waddlia chondrophila is an obligate intracellular bacterium considered as a potential agent of abortion in both humans and bovines. This member of the order Chlamydiales multiplies rapidly within human macrophages and induces lysis of the infected cells. To understand how this Chlamydia-like micro-organism invades and proliferates within host cells, we investigated its trafficking within monocyte-derived human macrophages. Vacuoles containing W. chondrophila acquired the early endosomal marker EEA1 during the first 30 min following uptake. However, the live W. chondrophila-containing vacuoles never co-localized with late endosome and lysosome markers. Instead of interacting with the endosomal pathway, W. chondrophila immediately colocalized with mitochondria and, shortly after, with endoplasmic reticulum-(ER-) resident proteins such as calnexin and protein disulfide isomerase. The acquisition of mitochondria and ER markers corresponds to the beginning of bacterial replication. It is noteworthy that mitochondrion recruitment to W. chondrophila inclusions is prevented only by simultaneous treatment with the microtubule and actin cytoskeleton-disrupting agents nocodazole and cytochalasin D. In addition, brefeldin A inhibits the replication of W. chondrophila, supporting a role for COPI-dependent trafficking in the biogenesis of the bacterial replicating vacuole. W. chondrophila probably survives within human macrophages by evading the endocytic pathway and by associating with mitochondria and the ER. The intracellular trafficking of W. chondrophila in human macrophages represents a novel route that differs strongly from that used by other members of the order Chlamydiales.

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Section: Methodsmentioning

confidence: 99%

Early intracellular trafficking of Waddlia chondrophila in human macrophages

Croxatto

Greub

2010

Microbiology

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“…Cells at 2 ϫ 10 6 /ml were incubated in a 1-ml volume for 1 h at 37ЊC in flat-bottom 24-well culture plates (Nunc). Monocytes were obtained by removing nonadherent cells and identified by previously described criteria (22). More than 95% of adherent cells were monocytes since they expressed CD14 and ingested zymosan particles.…”

Section: Patientsmentioning

confidence: 99%

Upregulation of tumor necrosis factor alpha and interleukin-1 beta in Q fever endocarditis

et al. 1996

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The occurrence of Q fever endocarditis likely involves some alterations in the responses of monocytes, the in vivo targets of Coxiella burnetii. To test this hypothesis, the production of the inflammatory cytokines tumor necrosis factor alpha, interleukin-1␤, and interleukin-6 was assessed in monocytes from patients with Q fever endocarditis. Spontaneous transcription and secretion of tumor necrosis factor and interleukin-1 were significantly higher in patient monocytes than in healthy controls. The interleukin-6 transcripts were also upregulated in patient cells. Moreover, in patients with recent endocarditis exhibiting high titers of immunoglobulin G directed to C. burnetii in phase I, monocytes released significantly higher levels of tumor necrosis factor and interleukin-1 than in patients with stabilized endocarditis. Immunoglobulin G titers and the overproduction of tumor necrosis factor and interleukin-1 were significantly correlated. Hence, the overproduction of inflammatory cytokines might be a marker of disease activity.

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“…The involvement of protein kinase C and protein tyrosine kinase in signalling leading to the synthesis and secretion of TNF-have been tested [16]. Two putative signal transduction pathways leading to cytokine synthesis for LPS and NLD and/or zymosan were suggested [17].…”

Section: Discussionmentioning

confidence: 99%

“…Isolated fractions of Nocardia were shown to exert adjuvant effect [2], to induce regression of experimental tumours [3][4][5][6][7], to enhance protection against irradiation [8,9], and to promote resistance to infections [10]. These activities include activation of natural killer (NK) cells and macrophages, followed by enhanced cytotoxicity of these cells and production of tumour necrosis factor-alpha (TNF-), IL-1 and other cytokines [3,[11][12][13][14][15][16][17] and stimulation of B cells to proliferation and differentiation into immunoglobulin-secreting cells [1,2,10,18,19].…”

Section: Introductionmentioning

confidence: 99%

Macrophage nitric oxide synthase (NOS) activation by Nocardia opaca fractions and 15- and 56-kD isolated antigens

Tučková

Zı́dek

Hanikýřová

et al. 1996

Clinical and Experimental Immunology

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SUMMARYThe Gram-positive bacterium, Nocardia opaca, is a source of substances with adjuvant effect, ability to stimulate macrophages and natural killer cells for enhanced cytotoxity and cytokine production and B lymphocytes for polyclonal immunoglobulin secretion. We determined the immunogenicity of isolated N. opaca fractions and prepared MoAbs against immunogenic water-soluble mitogen (NWSM). Two main proteins of molecular mass 15 and 56 kD were detected in Western blot analysis and isolated by affinity chromatography using anti-NWSM MoAb B7/7. Both these isolated nocardial antigens were found to stimulate mouse peritoneal macrophage NOS. The effect of 5 ¹ g NWSM was comparable to that of 5 ¹ g lipopolysaccharide (LPS) or 20 U of interferon-gamma (IFN-°) added to cell cultures. The MoAb B7/7 decreased NO 2 ÿ production induced by NWSM or by isolated nocardial antigens, but did not significantly influence the production elicited by LPS or IFN-°. On the other hand, NOS activation by NWSM was not affected by anti-IFN-°MoAb. The possible independent pathway for IFN-°and NWSM macrophage activation is discussed.

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Nocardia fractions, NLD and NWSM, induce tumor necrosis factor‐α secretion in human monocytes: Role of protein kinase C

Cited by 14 publications

References 33 publications

Early intracellular trafficking of Waddlia chondrophila in human macrophages

Early intracellular trafficking of Waddlia chondrophila in human macrophages

Upregulation of tumor necrosis factor alpha and interleukin-1 beta in Q fever endocarditis

Macrophage nitric oxide synthase (NOS) activation by Nocardia opaca fractions and 15- and 56-kD isolated antigens

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