Nkx1.2 deletion decreases fat production in zebrafish
Xinyuan Wang,
Xinyi Li,
Yunsheng Wang
et al.
Abstract:ObjectiveThis study aimed to investigate the role of Nkx1‐2, a transcription factor with the NK homeobox domain, in the regulation of fat production.MethodsGene expression was analyzed using quantitative real‐time polymerase chain reaction or transcriptome sequencing. CRISPR/Cas9 technology was employed to generate nkx1.2 knockout zebrafish and nkx1.2‐deleted 3T3‐L1 cells. Lipid droplet production in zebrafish larvae was visually quantified using Nile red staining, whereas lipid droplets in 3T3‐L1 cells were s… Show more
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