<i>Nicotiana langsdorffii</i>wild type and genetically modified exposed to chemical and physical stress: changes in element content

Ranaldo, Martina; Toscano, Giuseppa; Radaelli, Marta; Scalabrin, Elisa; Capodaglio, Gabriele

doi:10.1080/03067319.2015.1016013

Cited by 7 publications

(9 citation statements)

References 33 publications

(24 reference statements)

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“…Regarding elemental content, we observed the diminution of Na, K, Ca, Mn, Fe, and Ag. This result is in agreement with previous studies regarding Nicotiana langsdorffii modified by the insertion of rolC and GR and exposed to abiotic stresses [ 10 , 21 , 23 ]. The diminution of elements in WS plants could indicate a reduced efficiency of nutrient uptake [ 77 ].…”

Section: Resultssupporting

confidence: 93%

“…The sample treatment procedure followed the protocol already reported by Ranaldo et al [23]. Aliquots of about 0.1 g of lyophilized samples were digested in 6 mL of ultrapure nitric acid (65%) and 4 mL of hydrogen peroxide (33%) using a temperature-controlled microwave oven (Ethos1-Milestone S.r.l.…”

Section: Elemental Analysismentioning

confidence: 99%

“…The instrument was fitted out with a concentric nebulizer, a Peltier-cooled quartz spray chamber, and a quartz torch with a quartz injector tube. Optimization of instrumental performance, tuning details, and operating conditions have been previously reported [23]. Rhodium was used as an internal standard.…”

Section: Elemental Analysismentioning

confidence: 99%

“…This work presents the results of a high-throughput metabolomics and ionomics study of rolD transgenic N. langsdorffii plants exposed to water stress (WS) and high temperature stress (HS). Wild-type plants and GR and rolC genetically modified N. langsdorffii plants have already been considered in previous studies [14,15,23]. Our aim was to identify the influence of abiotic stresses on plants and to highlight the effects of rolD genetic modification on plant stress response.…”

Section: Introductionmentioning

confidence: 99%

“…The rol genes from Agrobacterium rhizogenes , when naturally engineered in plants, have been shown to induce multiple physiological and biochemical alterations in transformed plants [ 16 , 17 ], activating secondary metabolic processes and enhancing the plant’s response to abiotic and biotic stresses [ 16 , 17 , 18 , 19 ]. The Nicotiana genus (family of Solanaceae ) has been extensively investigated in genetic and physiological studies; in particular, the integration of rolC and GR genes in N. langsdorffii and N. glauca has shown interesting results for the improvement of plant resistance to different abiotic stresses [ 10 , 12 , 14 , 15 , 18 , 20 , 21 , 22 , 23 ]. Among the rol genes from A. rhizogenes , rolD has been investigated to a lesser extent.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes

2020

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Abiotic stresses are major factors that negatively affect plant growth and productivity. Plants have developed complex strategies to ensure their survival and reproduction under adverse conditions, activating mechanisms that involve changes at different metabolic levels. In order to select stress-resistant species, research has focused on molecular studies and genetic engineering, showing promising results. In this work, the insertion of the rolD gene from Agrobacterium rhizogenes into Nicotiana langsdorffii plants is investigated, in order to assess the potential of this genetic modification towards mitigating water and heat stresses. Different approaches were combined: a high-throughput metabolomics and ionomics study was performed, together with the determination of important plant phytohormones. The aim was to identify the influence of abiotic stresses on plants and to highlight the effects of the rolD genetic modification on plant stress response. The most relevant compounds for each kind of stress were identified, belonging mainly to the classes of lipids, acyl sugars, glycosides, and amino acid derivatives. Water stress (WS) determined a decrease of elements and secondary metabolites, while amino acids and their derivatives increased, proving to be key molecules in this type of stress. RolD plants exposed to high temperature stress (HS) presented higher dry weight levels than controls, as well as increased amounts of K and adenosine and lower levels of damage-associated metabolites, suggesting the increased resistance of rolD-modified plants toward HS.

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Section: Resultssupporting

confidence: 93%

Section: Elemental Analysismentioning

confidence: 99%

Section: Elemental Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes

2020

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Distribution Pattern of Rare Earth Elements in Four Different Industrial Hemp Cultivars (Cannabis sativa L.) Grown in Friuli Venezia Giulia, Italy

Calvi,

Corami,

Radaelli

et al. 2024

J Soil Sci Plant Nutr

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Simultaneous determination of shikimic acid, salicylic acid and jasmonic acid in wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses

Scalabrin

Radaelli

Capodaglio

2016

Plant Physiology and Biochemistry

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The presence and relative concentration of phytohormones may be regarded as a good indicator of an organism's physiological state. The integration of the rolC gene from Agrobacterium rhizogenes and of the rat glucocorticoid receptor (gr) in Nicotiana langsdorffii Weinmann plants has shown to determine various physiological and metabolic effects. The analysis of wild and transgenic N. langsdorffii plants, exposed to different abiotic stresses (high temperature, water deficit, and high chromium concentrations) was conducted, in order to investigate the metabolic effects of the inserted genes in response to the applied stresses. The development of a new analytical procedure was necessary, in order to assure the simultaneous determination of analytes and to obtain an adequately low limit of quantification. For the first time, a sensitive HPLC-HRMS quantitative method for the simultaneous determination of salicylic acid, jasmonic acid and shikimic acid was developed and validated. The method was applied to 80 plant samples, permitting the evaluation of plant stress responses and highlighting some metabolic mechanisms. Salicylic, jasmonic and shikimic acids proved to be suitable for the comprehension of plant stress responses. Chemical and heat stresses showed to induce the highest changes in plant hormonal status, differently affecting plant response. The potential of each genetic modification toward the applied stresses was marked and particularly the resistance of the gr modified plants was evidenced. This work provides new information in the study of N. langsdorffii and transgenic organisms, which could be useful for the further application of these transgenes.

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Nicotiana langsdorffiiwild type and genetically modified exposed to chemical and physical stress: changes in element content

Cited by 7 publications

References 33 publications

Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes

Effects of Water Deficit and Heat Stress on Nicotiana langsdorffii Metabolomic Pattern Modified by Insertion of rolD Gene from Agrobacterium rhizogenes

Distribution Pattern of Rare Earth Elements in Four Different Industrial Hemp Cultivars (Cannabis sativa L.) Grown in Friuli Venezia Giulia, Italy

Simultaneous determination of shikimic acid, salicylic acid and jasmonic acid in wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses

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