Nerium oleanderDistillate Improves Fat and Glucose Metabolism in High-Fat Diet-Fed Streptozotocin-Induced Diabetic Rats

Abstract

Diabetes was induced by intraperitoneal injection of streptozotocin (35 mg/kg bw) in all rats of five groups after being fed for 2 weeks high-fat diet. Type 2 diabetic Nerium-oleander- (NO-) administered groups received the NO distillate at a dose of 3.75, 37.5, and 375 μg/0.5 mL of distilled water (NO-0.1, NO-1, NO-10, resp.); positive control group had 0.6 mg glibenclamide/kg bw/d by gavage daily for 12 weeks. Type 2 diabetic negative control group had no treatment. NO distillate administration reduced fasting blood glucose, HbA1c, insulin resistance, total cholesterol, low density lipoprotein, atherogenic index, triglyceride-HDL ratio, insulin, and leptin levels. Improved beta cell function and HDL concentration were observed by NO usage. HDL percentage in total cholesterol of all NO groups was similar to healthy control. NO-10 distillate enhanced mRNA expressions of peroxisome proliferator-activated-receptor- (PPAR-) α, β, and γ in adipose tissue and PPAR-α–γ in liver. The findings from both in vivo and in vitro studies suggest that the considerable beneficial effect of NO distillate administration at a dose of 375 μg/0.5 mL of distilled water may offer new approaches to treatment strategies that target both fat and glucose metabolism in type 2 diabetes.

Exploring a natural MDR reversal agent: potential of medicinal food supplement Nerium oleander leaf distillate

“…The effects of N. oleander distillate as food supplement on rats taking high fat diet were investigated also by the same group. It was found that N. oleander distillate may be used as medicinal food supplement for treatment of hypercholesterolemia in diabetic rats [10] .…”

“…According to XTT tests, N. oleander distillate is not toxic for MCF-7/S, MCF-7/Pac and MCF-7/Vinc cells when in vivo concentration applied to animals are considered [10] . Also the effects of distillate on viability of all cell lines were similar (IC 50 0.22 mg/mL).…”

“…Zhao et al declared that the cardenolide compounds extracted from N. oleander exhibited MDR modulator effect on doxorubicin resistant ovarian carcinoma cell line 2780AD [9] . Bas et al reported that N. oleander distillate may be used as dietary supplement to treat high blood cholesterol level in diabetic rats [10] . Yazihan et al also suggested that N. oleander distillate is not toxic to non carcinoma cells [11] .…”

Hypoglycemia Associated With Oleander Toxicity in a Dog

“…In an experimental rat model, Mwafy and Yassin (2011) [5] demonstrated lower blood glucose levels in diabetic rats treated with Nerium oleander extract compared with controls. In another study [6], experimentally induced diabetic rats had improved pancreatic beta cell function when administered Nerium oleander distillate, suggesting insulin secretagogue and sensitizing effects of the extract. Further analysis by spectrometric analysis [7] has identified chlorogenic acid in Nerium leaves, a noncompetitive inhibitor of alpha-glucosidase.…”

“…Nerium oleander plant extracts have been under investigation as hypoglycemic agents in the study of diabetes mellitus in experimental animal models [5,6]. In an experimental rat model, Mwafy and Yassin (2011) [5] demonstrated lower blood glucose levels in diabetic rats treated with Nerium oleander extract compared with controls.…”