Neisseria gonorrhoeaemulti-antigen sequence typing (NG-MAST) of ciprofloxacin resistant isolates of Pretoria, South Africa

Abstract

Gonococcal isolates resistant to ciprofloxacin collected from 2004 to 2005, in the Pretoria region, were characterised using Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST). The isolates were obtained from men presenting with urethritis to primary healthcare clinics and general practitioners. All isolates were tested for susceptibility to ciprofloxacin by Etest, disc diffusion and agar dilution methods. Sequence-based typing, directed at the por and tbpB genes, and compared with international isolates using the NG-MAST database on the internet, was done for 18 isolates (10 ciprofloxacin-resistant isolates and 8 susceptible controls). There was one cluster (four isolates) of known sequence type (ST) similar to the pattern seen among strains from Scotland, England, and Durban, South Africa. Two other known STs were identified, while the remaining STs were unique.

Typing of Neisseria gonorrhoeae Opa and NG-MAST gene of 12 pairs of sexual contact gonorrhea patients in China

“…Neisseria gonorrhoeae MAST bank has been established, strains isolated from different regions can be compared on the Internet. Thus, this typing method is superior to other typing methods in that it can be used for not only short-term local epidemiological investigation of a outbreak but also long-term epidemiological study [16][17][18] .…”

Molecular epidemiology of gonorrhoea in Wales (UK)

“…NG-MAST is a highly discriminatory sequence based typing method4 which examines sequence variation in two hypervariable genes ( tpbB and porB ) and has been used successfully to investigate sexual networks in Sheffield,5 London,6 Scotland,7 Sweden8 and South Africa,9 together with antimicrobial susceptibility profiles.…”