<i>NAS</i>
            Overexpression and Rice Zinc Biofortification

Intrinsic improvement of Fe concentration in the rice grains, called rice Fe biofortification, is a promising countermeasure against widespread human Fe deficiency. In this study, two novel rice Fe biofortification approaches are reported. The first approach (Y approach) involved the expression of maize YELLOW STRIPE 1 controlled by the HEAVY METAL ATPASE 2 promoter. Y approach increased the polished grain Fe concentrations up to 4.8-fold compared to the non-transgenic (NT) line. The second approach (T approach) involved the expression of rice TRANSPORTER OF MUGINEIC ACID 1 controlled by the FERRIC REDUCTASE DEFECTIVE LIKE 1 promoter. T approach increased the polished grain Fe concentrations by up to 3.2-fold. No synergistic increases in the polished grain Fe concentrations were observed when Y and T approaches were combined (YT approach). However, the polished grain Fe concentrations further increased by 5.1- to 9.3-fold compared to the NT line, when YT approach was combined with the endosperm-specific FERRITIN expression (YTF approach) or when YTF approach was combined with the constitutive NICOTIANAMINE SYNTHASE expression (YTFN approach). Total grain weight per plant in most Y, T, YT and YTFN lines was comparable to that in the NT line, while it was significantly decreased in most YTF lines.

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NAS Overexpression and Rice Zinc Biofortification

Cited by 3 publications

References 67 publications

Molecular mechanistic approaches for iron and zinc biofortification in field plants

Molecular mechanistic approaches for iron and zinc biofortification in field plants

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