N -Succinyltransferase Encoded by a Cryptic Siderophore Biosynthesis Gene Cluster in Streptomyces Modifies Structurally Distinct Antibiotics

Abstract

In their natural habitats, bacteria encounter a plethora of organic compounds, some of which may be represented by antibiotics produced by certain members of the microbial community. A number of antibiotic resistance mechanisms have been described, including those specified by distinct genes encoding proteins that degrade, modify, or expel antibiotics.

Microbial communities associated with marine sponges from diverse geographic locations harbour biosynthetic novelty

“…17,50,83,84 During binning, contigs, and thus BGCs, may not be attributed to the correct MAG or as is more commonly the case not be attributed to any MAG. 78 While a BGC typically contains most or all of the biosynthetic enzymes, regulatory genes, resistance elements and transporters required for compound production, 85 unusual genes or gene arrangements including the use of genes that are not co-localised may occur 86,87 , which may further complicate correct attribution. The pipeline presented here allows facile processing of numerous datasets and facilitates selection of samples containing taxonomic groups and/or BGCs of interest based on relatively inexpensive shotgun sequencing.…”