N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion fromD,L-2-Amino-Δ²-Thiazoline-4-Carboxylic Acid toL-Cysteine byPseudomonassp. ON-4a

Identification, Cloning, and Sequencing of the Genes Involved in the Conversion ofD,L-2-Amino-Δ2-Thiazoline-4-Carboxylic Acid to …

“…As shown in our previous work, 5) in the conversion of D,L-ATC to L-cysteine in Pseudomonas sp. ON-4a and other ATC-assimilating bacteria, L-NCC was detected as an intermediate and L-cysteine was formed from L-NCC.…”

“…It is thus considered that only L-ATC in D,L-ATC is converted to L-cysteine by two Lspeciˆc enzymes of ATC hydrolase and NCC amidohydrolase in these bacterial strains. 5) In this study, we cloned a 3.4-kb DNA fragment from strain ON-4a containing the genes for these two enzymes involved in the conversion of D,L-ATC to L-cysteine, ATC hydrolase, and NCC amidohydrolase.…”

“…16,19,21,[24][25][26] These results suggest that there are 2 classes of N-carbamoyl amino acid amidohydrolases, N-carbamoyl-D-and N-carbamoyl-L-amino acid amidohydrolases. NCC amidohydrolase hydrolyzed only the L-form of NCC, 5) and showed signiˆcant similarity to the known N-carbamoyl-L-amino acid amidohydrolases (Fig. 3), suggesting that NCC amidohydrolase is a member of the N-carbamoyl-Lamino acid amidohydrolase class.…”

“…Similar results were obtained with ATC-assimilating bacteria, such as Pseudomonas putida AJ3865. 5) It therefore appears that L-NCC is an intermediate in the conversion of D,L-ATC to L-cysteine in these Pseudomonas strains. Furthermore, Shiba and Shiba 6) recently reported the isolation of a gene encoding an ATC racemase from Pseudomonas ovalis strain BS, which catalyzes the interconversion reaction between D-and L-forms of ATC.…”

“…ON-4a isolated by us, 5) and found that L-cysteine and N-carbamoyl-L-cysteine (L-NCC) were produced from D,L-ATC by crude enzymes from this strain. Similar results were obtained with ATC-assimilating bacteria, such as Pseudomonas putida AJ3865.…”