N‐acetylglucosaminyl N‐acetylmuramyl‐dipeptide, a novel murein building block formed during the cell division cycle of Proteus mirabilis

Release of cell wall peptides into culture medium by exponentially growing Escherichia coli

“…After permeabilization with ether (14), the cells (40 Ixl; 0.6 mg) were added to 160 ,ul of incubation buffer. The reaction mixture contained (final concentration) 50 mM Tris (pH 8.3), 50 mM NH4Cl, 20 mM MgCl2, 0.5 mM mercaptoethanol, 8 nmol of [3H]UDP-pentapeptide, and 4 nmol of UDP-N-acetylglucosamine (GlcNAc) (Sigma Chemical Co., St. Louis, Mo.). The samples were incubated for 60 min at 300; the reaction was stopped by boiling in 4% SDS for 30 min.…”

Characterization of minor fragments after digestion of escherichia coli murein with endo‐N,O‐diacetylmuramidase from chalaropsis, and determination of glycan chain length

“…This monomer species had not been described in E. coli, but was found also in Proteus mikbilis [ 51. The significance of such a monomer has been discussed [5].…”