<i>Mycoplasma gallisepticum</i>16S rRNA genes

Skamrov, A. V.; Gol’dman, M. A.; Klasova, Jana; Beabealashvilli, Robert Sh.

doi:10.1111/j.1574-6968.1995.tb07543.x

Cited by 9 publications

(2 citation statements)

References 7 publications

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“…Since the 16S rRNA gene is a highly conserved gene and ubiquitous in bacteria and archae, the similarity in this region is high with less variation. Previous research has used this gene region to identify a wide diversity of microbes within a single sample and single workflow and identification of taxa present in the sample [ 26 , 27 , 30 , 31 ]. This study observed an evolutionary relationship among all isolates.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in selected chicken breeds in South Africa

Idowu,

Mpofu,

Zishiri

et al. 2024

BMC Infect Dis

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Background The impact of chickens on maintaining the economy and livelihood of rural communities cannot be overemphasized. In recent years, mycoplasmosis has become one of the diseases that affect the success of South African chicken production. Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most prevalent strains of Mycoplasma in South Africa. MG and MS are significant respiratory pathogens affecting the productivity of chickens. The present study aimed to molecularly detect using qPCR and characterize the presence of MG and MS using phylogenetic analysis. The phylogenetic analysis was utilized to clarify general evolutionary relationships between related taxa of different MG and MS observed in tracheal swabs from South African chicken breeds. Methods Forty-five tracheal swabs of the Lohmann Brown (n = 9), Rhode Island Red (n = 9), Ovambo (n = 9), Venda (n = 9), and Potchefstroom Koekoek (n = 9) breeds were collected from symptomatic chickens present in the commercial farm. To detect MG and MS, DNA was extracted from tracheal swabs and faecal samples, and qPCR was performed with a 16 s rRNA (310 bp) and vlhA (400 bp) gene fragment. Following the sequencing of all the amplicons, MG, and MS dendrograms showing the evolutionary relationships among the five South African chicken breeds and the GeneBank reference population were constructed. Results The qPCR revealed the presence of MG and MS in 22% (2/9) of the tracheal swab samples tested for MS only in Rhode Island Red breeds; 66.6% (6/9) and 33% (3/9) of the tested samples in Ovambo breeds; and 11.1% (1/9) and 44.4% (4/9) of the tested samples in Venda breeds. No MG or MS were detected in the Lohmann Brown or Potchefstroom Koekoek breed. Furthermore, qPCR revealed the presence of MG in pooled faecal samples from Lohmann Brown and Ovambo breeds. Eight different bacterial isolates were recognized from both samples. Four isolates were of the 16 s ribosomal ribonucleic acid (rRNA) gene (named PT/MG51/ck/00, PT/MG48/ck/00, PT/MG41/ck/00 and PT/MG71/ck/00) gene of Mycoplasma gallisepticum, and the other was Mycoplasma Synoviae variable lipoprotein hemagglutinin A (vlhA) gene (named PT/MSA22/ck/01, PT/MS41/ck/01, PT/MS74/ck/01 and PT/MS46/ck/01) which were available in GenBank. These isolates were successfully sequenced with 95–100% similarity to the isolates from the gene bank. Conclusion The study revealed the presence of both MG and MS in the chicken breeds sampled. Furthermore, the different breeds of chicken were found to be susceptible to infection under the intensive or commercial management system. Therefore, continuous surveillance is encouraged to prevent the spread and outbreak of MG and MS in the poultry industry in South Africa.

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Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in selected chicken breeds in South Africa

Idowu,

Mpofu,

Zishiri

et al. 2024

BMC Infect Dis

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“…many biases have to be evaluated when genome copies are employed, as biological features of mycoplasma strain, phase of mycoplasma growth, growth conditions and mycoplasma sample handling after the cultivation 8 . Besides, the copy number of the 16s rRnA gene differs among the different species of mycoplasma, introducing as such a further bias to the genome copy number evaluation 39,40 . Additionally, the yield provided by the different DnA isolation methods may vary widely, generating outputs that do not correlate to the real bacterial amounts in the samples, if the DnA isolation method is changed.…”

Section: /13mentioning

confidence: 99%

Validação de método de PCR para detecção de micoplasmas no controle de qualidade da vacina de Febre Amarela

Lawson-Ferreira,

Santos,

Parmera

et al. 2016

Rev Inst Adolfo Lutz

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Dentre as vacinas produzidas por Bio-Manguinhos, um importante centro de produção de imunobiológicos da América Latina, destaca-se a vacina de febre amarela (FA) que é produzida em ovos embrionados. Para garantir a excelência e a segurança da vacina, testes de controle de qualidade são realizados durante a produção. A Organização Mundial de Saúde (OMS) exige dos produtores a ausência de Mycoplasma orale, M. pneumoniae, M. gallisepticum e M. synoviae em produtos biológicos. Micoplasmas são micro-organismos fastidiosos, sendo necessários 35 dias para que os testes de cultura sejam conclusivos. Neste estudo foram selecionados métodos de amplificação de fragmentos do gene 16SrRNA para detecção de micoplasmas em produtos intermediários da vacina de FA. Esta metodologia padronizada foi capaz de detectar baixas concentrações de micoplasmas nos produtos intermediários e a ausência de amplificação inespecífica foi demonstrada. Olimite de detecção variou entre 3,1 e 12,5 unidades formadoras de colônia; e nas amostras testadas a sensibilidade e a especificidade foram de 100 %. Oprotocolo de PCR para detecção de micoplasmas na vacina foi validado pela análise de 286 amostras. Bio-Manguinhos produz 10.000.000 doses de vacina de febre amarela por ano e, desde 2008, este método tem sido empregado com sucesso, complementando-se a abordagem tradicional.

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Muto

Ushida

2002

Molecular Biology and Pathogenicity of Mycoplasmas

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Mycoplasma gallisepticum16S rRNA genes

Cited by 9 publications

References 7 publications

Molecular detection and genetic characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in selected chicken breeds in South Africa

Molecular detection and genetic characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in selected chicken breeds in South Africa

Validação de método de PCR para detecção de micoplasmas no controle de qualidade da vacina de Febre Amarela

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