<i>Mycobacterium avium</i> Subsp.<i> paratuberculosis</i> Induces Specific IgE Production in Japanese People with Allergies

Cossu, Davide; Otsubo, Shigeru; Otsubo, Yuriko; Eda, Shigetoshi; Suzuki, Tsuyoshi; Iwao, Y; Kuribayashi, Takashi; Yamamoto, Seigo; Sechi, Leonardo Antonio; Momotani, Eiichi

doi:10.1155/2017/7959154

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…Individuals exposed to Mycobacterium tuberculosis or Mycobacterium avium Subsp. Paratuberculosis infection generated IgE responses [32,33]. Although mycobacterial antigen is generally considered to downregulate IgE production [34], our and others' results implied that some mycobacterium including BCG Lymphocytes from the spleens and MLNs were stimulated with 10 μg/ml ConA or 10 μg/ml OVA.…”

Section: Discussionmentioning

confidence: 53%

Inhibition of uPAR-TGFβ crosstalk blocks MSC-dependent EMT in melanoma cells

et al. 2015

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Current treatments for allergic asthma primarily ameliorate symptoms rather than inhibit disease progression. Regulating the excessive T helper type 2 (Th2) responses may prevent asthma exacerbation. In this study, we investigated the protective effects of Ad5-gsgAM, an adenovirus vector carrying two mycobacterial antigens Ag85A and Mtb32, against allergic asthma. Using an ovalbumin (OVA)-induced asthmatic mouse model, we found that Ad5-gsgAM elicited much more Th1-biased CD4 + T and CD8 + T cells than bacillus CalmetteGuérin (BCG). After OVA challenge, Ad5-gsgAM-immunized mice showed significantly lowered airway inflammation in comparison with mice immunized with or without BCG. Total serum immunoglobulin E and pulmonary inducible-nitric-oxide-synthase were efficiently reduced. The cytokine profiles in bronchial-alveolar-lavage-fluids (BALFs) were also modulated, as evidenced by the increased level of interferon-γ (IFN-γ) and the decreased level of interleukin (IL)-4, IL-5, and IL-13. Anti-inflammatory cytokine IL-10 was sharply increased, whereas pro-inflammatory cytokine IL-33 was significantly decreased. Importantly, exogenous IL-33 abrogated the protective effects of Ad5-gsgAM, revealing that the suppression of IL-33/ST2 axis substantially contributed to protection against allergic inflammation. Moreover, regulatory T cells were essential for regulating aberrant Th2 responses as well as IL-33/ST2 axis. These results suggested that modulating the IL-33/ST2 axis via adenovirus-vectored mycobacterial antigen vaccination may provide clinical benefits in allergic inflammatory airways disease. Key messages•Ad5-gsgAM elicits Th1 responses and suppresses Th2-mediated allergic asthma in mice.•Ad5-gsgAM inhibits IL-33/ST2 axis by reducing IL-33 secretion but not ILC2 recruiting.•Treg is essential for modulating Th2 responses and IL-33/ST2 axis by Ad5-gsgAM.

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Section: Discussionmentioning

confidence: 53%

Inhibition of uPAR-TGFβ crosstalk blocks MSC-dependent EMT in melanoma cells

et al. 2015

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“…The results obtained in this study highlight the risk of the transmission of this pathogenic mycobacterium to the community. A higher exposition to MAP, which causes a persistent infection in its host [ 44 ], may lead to trigger not only inflammatory bowel diseases including Crohn’s disease [ 44 , 45 ], but also different autoimmune diseases, as it has been previously associated with in several studies [ 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. This should motivate reflection by legislators and encourage the adoption of the best policies to lower the leakage of this bacterium into communities.…”

Section: Discussionmentioning

confidence: 99%

Identification of Mycobacterium avium subsp. paratuberculosis (MAP) in Sheep Milk, a Zoonotic Problem

et al. 2020

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Johne’s disease (JD) is a life-threatening gastrointestinal disease affecting ruminants, which causes crucial economical losses globally. This ailment is caused by Mycobacterium avium subsp. paratuberculosis (MAP), a fastidious intracellular pathogen that belongs to the Mycobacteriaceae family. This acid-fast, hard-to-detect bacterium can resist milk pasteurization and be conveyed to dairy product consumers. Many studies have emphasized the zoonotic nature of MAP, suggesting an association between MAP and some gastroenteric conditions such as Crohn’s disease in humans. This underlines the importance of utilizing efficient pasteurization alongside a state-of-the-art diagnostic system in order to minimize the possible ways this pathogen can be conveyed to humans. Until now, no confirmatory MAP screening technique has been developed that can reveal the stages of JD in infected animals. This is partially due to the lack of an efficient gold-standard reference method that can properly evaluate the performance of diagnostic assays. Therefore, the following research aimed to compare the merits of qPCR and ELISA assessments of milk for the detection of MAP in a total of 201 Sardinian unpasteurized sheep milk samples including 73 bulk tank milk (BTM) and 128 individual samples from a MAP-infected flock (MIF) applying various reference models. Accordingly, milk qPCR and ELISA assessments, together and individually, were used as reference models in the herd-level study, while serum ELISA and fecal PCR were similarly (together and in isolation) considered as the gold standards in the individual-level diagnosis. This study showed that the type of gold-standard test affects the sensitivity and specificity of milk qPCR and ELISA significantly. At the individual level in the MAP-infected flock, serum ELISA in isolation and together with fecal PCR were recognized as the best references; however, the best correlation was seen between milk and serum ELISA (p < 0.0001). Regarding the detection of MAP in BTM, qPCR IS900 was recognized as the most sensitive and specific diagnostic test (p < 0.0001) for monitoring the MAP shedders and animals with clinically developed symptoms within herds, under the condition that both milk qPCR and milk ELISA tests formed a binary reference model. The BTM analyses (qPCR and ELISA) revealed that MAP positivity has a seasonal pattern. This hypothesis was proven through a longitudinal study on 14 sheep herds.

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“…IgE was increased in both indicated allergic reaction by adding adjuvant (Cossu et al, 2017). Elfiky et al (2023) by histopathology & immunology found that bee venom by intradermal injection in 0.1mg in aqueous solution was safe without side effects.…”

Section: Discussionmentioning

confidence: 98%

Investigation of Immunological Response of Egyptian Bee Venom Preparation in Rabbits

ELFIKY,

ABOUOUF

2023

Journal of the Egyptian Society of Parasitology

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The repeated exposure of the immune system to bee venom as antigen leads to significant increase in immune response. In this study different immunological schedules were tried to reach maximum immunological effect with the least allergic side effect either by using and aqueous or emulsified preparation of bee venom. Three groups of male rabbits of one year old each were used for a period of four weeks. The blood samples were with drown before bee venom injection and weekly after its infection for determination of IgG and IgE. The results showed that injection of Egyptian bee venom in an aqueous form have the ability to boost the level of immune response without significant allergic reaction.

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Mycobacterium avium Subsp. paratuberculosis Induces Specific IgE Production in Japanese People with Allergies

Cited by 5 publications

References 43 publications

Inhibition of uPAR-TGFβ crosstalk blocks MSC-dependent EMT in melanoma cells

Inhibition of uPAR-TGFβ crosstalk blocks MSC-dependent EMT in melanoma cells

Identification of Mycobacterium avium subsp. paratuberculosis (MAP) in Sheep Milk, a Zoonotic Problem

Investigation of Immunological Response of Egyptian Bee Venom Preparation in Rabbits

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