<i>MTHFR</i>
            rs1801133 Polymorphism and Susceptibility to Colorectal Cancer in Iranian Population: Evidence of a Case–Control Study and Meta-Analysis

Haerian, Monir Sadat; Haerian, Batoul Sadat; Molanaei, Saadat; Kosari, Farid; Sabeti, Shahram; Bidari‐Zerehpoosh, Farahnaz; Abdolali, Ebrahim; Zali, Mohammad Reza

doi:10.2217/pgs-2016-0048

Cited by 9 publications

(5 citation statements)

References 40 publications

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“…However, in the present study, we found an increase of CRC risk in MTHFR TT respectively reported that CC genotype has a protective effect on CRC [5,32]. Although a more recent combined case-control study and meta-analysis on 2421 subjects has shown no signi cant association between MTHFR C677T polymorphism and the risk of CRC in Iranian population suggesting the need for a bigger sample size for MTHFR association studies [33]. There are also some reports indicating the increased risk of TT genotype in other populations [34,35].…”

Section: Discussioncontrasting

confidence: 77%

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

Ghorbani

Azghandi

Khayyami

et al. 2021

Preprint

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Background: The incidence of colorectal cancer (CRC) has increased during recent years in Iran and other developing countries. Clinical studies suggest that essential folate dietary intake and moderate deficiency of methylenetetrahydrofolate reductase (MTHFR) may protect and reduce the risk of CRC.The aim of the present study was to investigate the clinical significance of C677T polymorphism within MTHFR gene and its correlation with the serum folate and Vit B12 in the Iranian population suffering from CRC.Methods: Blood samples were taken from 1017 Iranian individuals (517 cases and 500 controls) who were referred for colonoscopy. TaqMan probe assay was performed for C677T MTHFR polymorphism. Sera were fractionated from the blood samples of 43 patients and controls and folate and Vit B12 concentrations were measured by a monobind kit. The correlation of MTHFR polymorphisms and folate/vitamin-B12 with CRC risk were analyzed.Results: In the current study, we found the frequency of three different genotypes of MTHFR polymorphism in the Iranian population i.e., CC, CT and TT, to be 51.31, 26.73, 21.96 and 61, 32.2, 6.8 in case and control groups, respectively. The homozygote genotype of MTHFR rs1801133 polymorphism is associated with increased risk of CRC by 3.68, 1.42 and 3.74-fold in codominant, dominant and recessive models respectively (p value< 0.01). Our study revealed that there was no significant difference between the amount of folate and Vit B12 in case and control groups (p value> 0.05).Conclusions: This study revealed that there was no significant difference between the amount of folate and Vit B12 in the case and control groups. Furthermore, our results demonstrated a higher risk association for 677TT and 677TT+C677T genotypes of MTHFR compared with 677CC carriers among CRC patients.

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Section: Discussioncontrasting

confidence: 77%

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

Ghorbani

Azghandi

Khayyami

et al. 2021

Preprint

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“…According to PRISMA-P, this meta-analysis contained 37049 cases and 52444 controls that were combined from 87 publications with 91 eligible case–control studies to examine the relationship between rs1801133 polymorphism and CRC risks [ 26 – 112 ]. The literature retrieval and selection process are shown in the flowchart in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, there were several meta-analyses performed to elucidate the association of MTHFR C677T polymorphism and the susceptibility to CRC before [ 26 , 115 – 118 ]. Compared with them, this meta-analysis included the most eligible reported studies with the largest sample size and had no restrictions in ethnicity.…”

Section: Discussionmentioning

confidence: 99%

“…Zhong et al [ 118 ], carried out a meta-analysis obtaining similar results in East Asians and further subgroup analyses by country identified such association in Korea and Japan. Nevertheless, the recent meta-analysis failed to identify that rs1801133 polymorphism was connected with CRC susceptibility in Iranian population [ 26 ]. By means of stratified analysis based on the source of controls and genotyping methods, the positive results were observed in population-based control group and PCR-RFLP method.…”

Section: Discussionmentioning

confidence: 99%

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Methylenetetrahydrofolate reductase C677T polymorphism and colorectal cancer susceptibility: a meta-analysis

Qin

Wang

et al. 2017

Bioscience Reports

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The association between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and colorectal cancer (CRC) susceptibility has been researched in numerous studies. However, the results of these studies were controversial. Therefore, the objective of this meta-analysis was to offer a more convincible conclusion about such association with more included studies. Eligible studies published till May 1, 2017 were searched from PubMed, Embase, Web of Science, and CNKI database about such association. Pooled odds ratios (ORs) together with 95% confidence intervals (CIs) were calculated to evaluate such association. And the Begg’s funnel plot and Egger’s test were applied to assess the publication bias. This meta-analysis contained 37049 cases and 52444 controls from 87 publications with 91 eligible case–control studies. Because of lack of data for a particular genotype in several studies, all the included studies were analysed barely in the dominant model. Originally, there was no association between MTHFR C677T polymorphism and CRC susceptibility (OR =0.99, 95% CI =0.94–1.05). After excluding 13 studies according to their heterogeneity and publication bias, rs1801133 polymorphism was found to reduce the risks of CRC significantly (OR =0.96, 95% CI =0.94–0.99). In the subgroup analysis of ethnicity, there was a significant association in Asians (OR =0.94, 95% CI =0.89–1.00). Furthermore, when stratified by the source of controls and genotyping methods, the positive results were observed in population-based control group (OR =0.97, 95% CI =0.93–1.00) and PCR-restriction fragment length polymorphism (PCR-RFLP) method (OR =0.95, 95% CI =0.91–0.99. The results of the meta-analysis suggested that MTHFR C677T polymorphism was associated with CRC susceptibility, especially in Asian population.

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“…Similarly, two studies on the Iranian population on 406 (175 cases, 231 controls) and 491 (234 cases, 257 controls) subjects, respectively reported that CC genotype has a protective effect on CRC [5,32]. Although a more recent combined case-control study and meta-analysis on 2421 subjects have shown no significant association between MTHFR C677T polymorphism and the risk of CRC in the Iranian population suggesting the need for bigger sample size for MTHFR association studies [33]. There are also some reports indicating the increased risk of TT genotype in other populations [34,35].…”

Section: Discussionmentioning

confidence: 99%

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

et al. 2021

View full text Add to dashboard Cite

Background The incidence of colorectal cancer (CRC) has increased during recent years in Iran and other developing countries. Clinical studies suggest that essential folate dietary intake and moderate deficiency of methylenetetrahydrofolate reductase (MTHFR) may protect and reduce the risk of CRC. The present study aimed to investigate the clinical significance of C677T polymorphism within the MTHFR gene and its correlation with the serum folate and Vit B12 in the Iranian population suffering from CRC. Methods Blood samples were taken from 1017 Iranian individuals (517 cases and 500 controls) who were referred for colonoscopy. TaqMan probe assay was performed for C677T MTHFR polymorphism. Sera were fractionated from the blood samples of 43 patients and controls and folate and Vit B12 concentrations were measured by a monobind kit. The correlation of MTHFR polymorphisms and folate/vitamin-B12 with CRC risk was analyzed. Results In the current study, we found the frequency of three different genotypes of MTHFR polymorphism in the Iranian population i.e., CC, CT, and TT, to be 51.31, 26.73, 21.96 and 61, 32.2, 6.8 in case and control groups, respectively. The homozygote genotype of MTHFR rs1801133 polymorphism is associated with an increased risk of CRC by 3.68, 1.42, and 3.74-fold in codominant, dominant, and recessive models respectively (p value < 0.01). Our study revealed that there was no significant difference between the amount of folate and Vit B12 in the case and control groups (p value > 0.05). Conclusions This study revealed that there was no significant difference between the amount of folate and Vit B12 in the case and control groups. Furthermore, our results demonstrated a higher risk association for 677TT and 677TT + C677T genotypes of MTHFR compared with 677CC carriers among CRC patients.

show abstract

MTHFR rs1801133 Polymorphism and Susceptibility to Colorectal Cancer in Iranian Population: Evidence of a Case–Control Study and Meta-Analysis

Abstract: This study failed to identify an association between the rs1801133 and susceptibility to CRC in Iranian population.

Cited by 9 publications

References 40 publications

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

Methylenetetrahydrofolate reductase C677T polymorphism and colorectal cancer susceptibility: a meta-analysis

Association of MTHFR C677T variant genotype with serum folate and Vit B12 in Iranian patients with colorectal cancer or adenomatous polyps

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