Moringa oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide‐induced oxidative stress in vitro

Abstract

Bovine mammary epithelial cells (BMECs) of high‐producing dairy cows are subject to constant oxidative stress as a result of high metabolic rate and physiological adaptation to intensive farming. Moringa (Moringa oleifera) leaf has been proposed to have the antioxidant potential in scavenging free radicals due to the presence of flavonoids. In this study, we investigated the cytoprotective effects of moringa leaf flavonoids in alleviating oxidative stress in BMECs in vitro. Oxidative stress was established by exposing isolated BMECs to H2O2 for 2 hr. Doses of moringa leaf flavonoids were evaluated by treating BMECs for 12 hr. The optimal concentrations of H2O2 and moringa leaf flavonoids were 500 μmol/L and 1.0 mg/ml, respectively. The results showed that moringa leaf flavonoids increased the activities of superoxide dismutase, catalase, and glutathione peroxidase; and reduced malondialdehyde activity and intracellular accumulation of reactive oxygen species (ROS) in the H2O2‐induced oxidative stress system. A Hoechst33258 staining assay revealed that moringa leaf flavonoids decreased the apoptosis rate in BMECs, while leaving membrane integrity and nucleolar morphology unchanged. We concluded that moringa leaf flavonoids have the antioxidant capacity to effectively reduce oxidative stress in BMECs.

Moringa oleifera Leaf Extract Protects C2C12 Myotubes against H2O2-Induced Oxidative Stress

“…Given that MOLE exerts its protective antioxidant effect against H 2 O 2 -induced oxidative stress only in pretreated cells, we speculate that the activation of the Nrf2 pathway is particularly important in enabling cells to respond when subjected to oxidative stress promptly. In fact, our data on muscle cells are in agreement with recent reports on the effects of the different components of Brassicaceae , and Moringa oleifera in particular, on other cellular models exposed to oxidative stress [ 75 , 76 , 77 , 78 , 79 , 80 , 81 ].…”

Nutritional Value of Moringa oleifera Lam. Leaf Powder Extracts and Their Neuroprotective Effects via Antioxidative and Mitochondrial Regulation

“…However, pretreatments with the three assayed concentrations of Moringa oleifera significantly reduced hydrogen peroxide-induced ROS overproduction. Similar results were observed for moringa leaf flavonoids in bovine mammary epithelial cells against hydrogen peroxide and the compound 1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside from Moringa oleifera seeds against carbon against CCl 4 [ 43 , 44 ].…”

Effects of Moringa oleifera Leaf Extract on Diabetes-Induced Alterations in Paraoxonase 1 and Catalase in Rats Analyzed through Progress Kinetic and Blind Docking

“…Moringa oleifera phytochemicals can protect liver mitochondria from oxidative injury [ 24 ]. Moreover, M. oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide-induced oxidative stress in vitro [ 25 ]. In addition, M. oleifera leaves extract induces vasorelaxation via endothelium-dependent hyperpolarization and calcium channel blockade in mesenteric arterial beds isolated from L-NAME hypertensive rats [ 26 ].…”