Moringa oleifera ethanolic extract ameliorates the testicular dysfunction resulted from HFD‐induced obesity rat model

Abstract

In this study, we estimated the protective role of Moringa oleifera leaf ethanolic extract (MOLE) against obesity‐associated testicular dysfunction. Fifty male albino rats were randomly assigned to five groups (n = 10): Group I (basal diet), group II (basal diet plus MOLE orally), group III (high‐fat diet—HFD), group IV (HFD plus oral MOLE) and group V (HFD for 8 weeks followed by a basal diet plus oral MOLE for 6 weeks). The study duration extended for 14 weeks. Serum collected to investigate testosterone, FSH and LH levels. Testicular tissues were used to determine levels of SOD, glutathione, catalase and malondialdehyde. Semen was collected to estimate its quality (morphology, motility and concentration). Morphological changes in the testis were investigated by histopathological and immunohistochemical techniques. Compared with both control treatment and MOLE treatment, serum testosterone, FSH, LH, testicular enzymatic catalase, SOD, GSH, survivin immunoreactivity, sperm quality and testicular weight were all significantly decreased in rats treated with HFD, while there were significant increases in testicular malondialdehyde and caspase‐3 immunoreactivity. MOLE improved all harmful effects of HFD. Improvements were more pronounced in the protected (G 4) than the treated (G 5) group. MOLE could be a potential solution for obesity‐associated fertility problem.

The possible effect of crude phenolic-rich extract from mushroom (Pleurotus ostreatus) on infertility induced by streptozotocin in rats fed a high-fat diet as a model of type 2 diabetes

“…The combination of obesity and diabetes has been shown to hurt various aspects of sperm quality as well as hormone levels and testicular health, as observed in the study by Alkafafy et al (2021). These results are similar to previous studies using obese and diabetic rat models.…”

Exploring the application of sildenafil for high-fat diet-induced erectile dysfunction based on interleukin-18-mediated NLRP3/Caspase-1 signaling pathway

“… 23 The modeling method of HFD is similar to the formation principle of dyslipidemia caused by human diet, and the modeling method is convenient, which is the common method of fat modeling animals at present. 24 , 25 This study found that elevated serum levels of TC, TG, and LDL-C can lead to endothelial dysfunction, which is closely related to ED. The ICP/MAP data showed that there was a statistically significant difference between the ICP/MAP ratios of the ED and IL-18 groups and the control group.…”

Effect of Moringa Leaves Ethanolic Extract (Moringa oleifera, Lam.) on Testicles Histopathological Structure in Metabolic Syndrome Rat Model

“…Alkafafy et al showed that ethanolic extract of Moringa leaves at 300 mg/kg improved mouse testicular dysfunction with a high-fat diet-induced obesity model, which was indicated by enhancement in testosterone hormone, LH, and FSH levels. The results showed that there was a decrease in degenerative changes in spermatogenic cells along with an increase in cell count and sperm motility 30 . Jannah et al reported that rats who had diabetes mellitus experienced histopathological improvement after the administration of ethanolic extract of Moringa leaves at 200 mg/kg, 300 mg/kg, 400 mg/kg, and 500 mg/kg doses, with the best histopathological structure observed at 400 mg/kg.…”