<i>MOB1</i>, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy

Luca, Francis C.; Winey, Mark

doi:10.1091/mbc.9.1.29

Cited by 165 publications

(204 citation statements)

References 76 publications

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“…This further suggests that hMOB1 can function in different cell cycle phases, which is very similar to observations made for budding yeast Mob1p. In yeast, Mob1p plays a role in spindle pole body duplication (the yeast equivalent of centrosome duplication in multicellular organisms) [96] in addition to being essential for mitotic exit [10,11,32,33]. Nevertheless, despite this recent research progress, a role for hMOB1 in mitotic exit and cytokinesis has as yet to be established.…”

Section: Mammalian Hippo Signalling In the G2/m Phase Of The Cell Cyclementioning

confidence: 99%

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

Hergovich

Hemmings

2012

Seminars in Cell & Developmental Biology

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Over the past decade Hippo kinase signalling has been established as an essential tumour suppressor pathway controlling tissue growth in flies and mammals. All members of the Hippo core signalling cassette are conserved from yeast to humans, whereby the yeast analogues of Hippo, Mats and Lats are central components of the mitotic exit network and septation initiation network in budding and fission yeast, respectively. Here, we discuss how far core Hippo signalling components in Drosophila melanogaster and mammals have reported similar mitotic functions as already established for their highly conserved yeast counterparts.

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Section: Mammalian Hippo Signalling In the G2/m Phase Of The Cell Cyclementioning

confidence: 99%

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

Hergovich

Hemmings

2012

Seminars in Cell & Developmental Biology

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“…Several putative phosphorylation sites exist (four for casein kinase II: aa 29-32, 52-55, 101-104, 115-118; three for protein kinase C: aa 115-117, 138-140, 147-149; one for protein kinase A: aa 149-152). Finally, it may be interesting to note that phocein and the yeast protein mob1 share 22% identity and 47% homology over a stretch of 185 aa (Luca and Winey, 1998). Mob1p is implicated in the onset of septum formation in Schizosaccharomyces pombe (Salimova et al, 2000).…”

Section: Identification Of Phocein and Domain Predictionmentioning

confidence: 99%

Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Baillat

Moqrich

Castets

et al. 2001

MBoC

116

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Phocein is a widely expressed, highly conserved intracellular protein of 225 amino acids, the sequence of which has limited homology to the subunits from clathrin adaptor complexes and contains an additional stretch bearing a putative SH3-binding domain. This sequence is evolutionarily very conserved (80% identity between Drosophila melanogaster and human). Phocein was discovered by a yeast two-hybrid screen using striatin as a bait. Striatin, SG2NA, and zinedin, the three mammalian members of the striatin family, are multimodular, WD-repeat, and calmodulinbinding proteins. The interaction of phocein with striatin, SG2NA, and zinedin was validated in vitro by coimmunoprecipitation and pull-down experiments. Fractionation of brain and HeLa cells showed that phocein is associated with membranes, as well as present in the cytosol where it behaves as a protein complex. The molecular interaction between SG2NA and phocein was confirmed by their in vivo colocalization, as observed in HeLa cells where antibodies directed against either phocein or SG2NA immunostained the Golgi complex. A 2-min brefeldin A treatment of HeLa cells induced the redistribution of both proteins. Immunocytochemical studies of adult rat brain sections showed that phocein reactivity, present in many types of neurons, is strictly somato-dendritic and extends down to spines, just as do striatin and SG2NA.

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“…Thus, cells have developed a signal transduction pathway, named the mitotic exit network (MEN), to inactivate CDK after mitosis (Morgan, 1999). The components of the MEN pathway include protein kinases Cdc5, Cdc15, Dbf2, a GTPase Tem1, a phosphatase Cdc14, and a Dbf2 binding protein Mob1 Luca and Winey, 1998;Lee et al, 2001a). Phosphatase Cdc14, the key player in the MEN pathway, dephosphorylates Cdh1, an activator for APC (anaphase promoting complex), and subsequently activates the degradation of a Btype cyclin, Clb2.…”

Section: Introductionmentioning

confidence: 99%

“…Thus we examined if overexpression of PP2A components is toxic to other temperature-sensitive mutants of the MEN pathway. P GAL -CDC55, P GAL -PPH21, P GAL -PPH22, P GAL -PPH3, and a control vector were introduced into tem1-3 and mob1-77 mutants that have defects in mitotic exit (Shirayama et al, 1994;Luca and Winey, 1998). The growth of the transformants was examined after incubation on both glucose and galactose plates at the permissive temperature.…”

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confidence: 99%

Phosphatase 2A Negatively Regulates Mitotic Exit inSaccharomyces cerevisiae

Wang

2006

MBoC

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In budding yeast Saccharomyces cerevisiae, Cdc5 kinase is a component of mitotic exit network (MEN), which inactivates cyclin-dependent kinase (CDK) after chromosome segregation. cdc5-1 mutants arrest at telophase at the nonpermissive temperature due to the failure of CDK inactivation. To identify more negative regulators of MEN, we carried out a genetic screen for genes that are toxic to cdc5-1 mutants when overexpressed. Genes that encode the B-regulatory subunit (Cdc55) and the three catalytic subunits (Pph21, Pph22, and Pph3) of phosphatase 2A (PP2A) were isolated. In addition to cdc5-1, overexpression of CDC55, PPH21, or PPH22 is also toxic to other temperature-sensitive mutants that display defects in mitotic exit. Consistently, deletion of CDC55 partially suppresses the temperature sensitivity of these mutants. Moreover, in the presence of spindle damage, PP2A mutants display nuclear localized Cdc14, the key player in MEN pathway, indicative of MEN activation. All the evidence suggests the negative role of PP2A in mitotic exit. Finally, our genetic and biochemical data suggest that PP2A regulates the phosphorylation of Tem1, which acts at the very top of MEN pathway. INTRODUCTIONThe key driving force for cell division in all eukaryotic cells is the conserved cyclin-dependent kinase (CDK). CDK activity fluctuates during the cell cycle, peaking at the S and M phases and dropping at the G1 phase. Although the high CDK activity is required for DNA duplication and chromosome segregation, the low CDK activity at late M and G1 phase is essential for the loading of DNA replication complexes onto replication origins (Noton and Diffley, 2000;Lengronne and Schwob, 2002). Thus, cells have developed a signal transduction pathway, named the mitotic exit network (MEN), to inactivate CDK after mitosis (Morgan, 1999). The components of the MEN pathway include protein kinases Cdc5, Cdc15, Dbf2, a GTPase Tem1, a phosphatase Cdc14, and a Dbf2 binding protein Mob1 Luca and Winey, 1998;Lee et al., 2001a). Phosphatase Cdc14, the key player in the MEN pathway, dephosphorylates Cdh1, an activator for APC (anaphase promoting complex), and subsequently activates the degradation of a Btype cyclin, Clb2. Cdc14 also enhances the stability of Sic1 protein, which acts as a CDK inhibitor (Visintin et al., 1998). During most of the cell cycle, Cdc14 is localized in the nucleolus, but its translocation out of the nucleolus in anaphase and telophase allows it to encounter its substrates, such as Cdh1 and Sic1. All the components in the MEN pathway are required for the release of Cdc14 from the nucleolus, suggesting that Cdc14 acts downstream of MEN pathway (Shou et al., 1999;Visintin et al., 1999).The regulation of MEN activity is achieved through the multilayer control of Tem1, a small GTPase that localizes at the spindle pole body (SPB) and acts on the very top of the MEN pathway. Tem1's activator, the GTPase exchange factor (GEF) Lte1, exhibits daughter-cell specific localization. Thus, SPB-localized Tem1 is activated after it encounters...

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MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy

Cited by 165 publications

References 76 publications

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

Hippo signalling in the G2/M cell cycle phase: Lessons learned from the yeast MEN and SIN pathways

Molecular Cloning and Characterization of Phocein, a Protein Found from the Golgi Complex to Dendritic Spines

Phosphatase 2A Negatively Regulates Mitotic Exit inSaccharomyces cerevisiae

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