<i>Miscanthus</i> as cellulosic biomass for bioethanol production

Lee, Wen‐Chien; Kuan, Wei‐Chih

doi:10.1002/biot.201400704

Cited by 111 publications

(79 citation statements)

References 66 publications

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“…The ethanol yield form Napier grass obtained in the present work (0.143 g/g dried raw material) is very close to that from others grasses. SSF of pretreated Miscanthus × giganteus and Miscanthus lutarioriparius results in experimental ethanol yields of 0.13 and 0.15 g/g‐raw material, respectively …”

Section: Resultsmentioning

confidence: 99%

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai

Lee

Kuan

et al. 2018

Energy Science & Engineering

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Due to its fast‐growing and high carbohydrate content, Napier grass has a great potential to be chosen as a raw material for renewable energy production. The Napier grass after different pretreatments was tested for simultaneous saccharification and fermentation (SSF) with dried yeast (Saccharomyces cerevisiae) and cellulase (CTec2) to produce ethanol. For alkaline pretreatment, the grass was incubated with 10% NaOH at a ratio of 1:20 (w/v) at 90°C for 1 hour. For dilute acid pretreatment, the grass was soaked with 1% H2SO4 at a ratio of 1:10 (w/v) and incubated at 120°C for 1 hour. For the two‐stage pretreatment, the resultant solid from dilute acid pretreatment was further treated with 2% NaOH (1:10 w/v) at 80°C for 6 hours. After enzymatic saccharification at 50°C and pH 5.0 for 96 hours using CTec2, yields of glucose from three pretreated Napier grass samples (10% water‐insoluble solids, WIS) were 75.4 ± 2.9%, 55.4 ± 6.5%, and 76.4 ± 5.0%. Using 10% WIS of alkaline‐pretreated Napier grass biomass as the substrate, the 72‐hour SSF led to an ethanol yield of 86.6 ± 3.4%. On the basis of the dried biomass of Napier grass as the raw material, the ethanol yield could reach 0.143 ± 0.006 (g/g). Napier grass biomass could be effectively treated by alkaline, dilute acid, or two‐stage pretreatment methods to remove non‐cellulosic components to some extent. Alkaline pretreatment was found to be superior to dilute acid and two‐stage pretreatment methods, based on ethanol yields obtained under similar SSF conditions.

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Section: Resultsmentioning

confidence: 99%

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Tsai

Lee

Kuan

et al. 2018

Energy Science & Engineering

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“…Miscanthus has been regarded as a promising bioenergy crop for lignocellulosic biofuel production due to its superior characteristics such as high biomass yield, perennial growth habitus, low water and fertilizer requirement, abiotic stress tolerance and broad adaptation to marginal land etc. (Lewandowski et al, 2000; Parveen et al, 2013; Robson et al, 2013; Lee and Kuan, 2015; Xing et al, 2016). The Miscanthus genus contains more than 14 species, most of which originate from southern and eastern Asia (Hodkinson et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

De novo Transcriptome Analysis of Miscanthus lutarioriparius Identifies Candidate Genes in Rhizome Development

Wang

et al. 2017

Front. Plant Sci.

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HIGHLIGHT De novo transcriptome profiling of five tissues reveals candidate genes putatively involved in rhizome development in M. lutarioriparius.Miscanthus lutarioriparius is a promising lignocellulosic feedstock for second-generation bioethanol production. However, the genomic resource for this species is relatively limited thus hampers our understanding of the molecular mechanisms underlying many important biological processes. In this study, we performed the first de novo transcriptome analysis of five tissues (leaf, stem, root, lateral bud and rhizome bud) of M. lutarioriparius with an emphasis to identify putative genes involved in rhizome development. Approximately 66 gigabase (GB) paired-end clean reads were obtained and assembled into 169,064 unigenes with an average length of 759 bp. Among these unigenes, 103,899 (61.5%) were annotated in seven public protein databases. Differential gene expression profiling analysis revealed that 4,609, 3,188, 1,679, 1,218, and 1,077 genes were predominantly expressed in root, leaf, stem, lateral bud, and rhizome bud, respectively. Their expression patterns were further classified into 12 distinct clusters. Pathway enrichment analysis revealed that genes predominantly expressed in rhizome bud were mainly involved in primary metabolism and hormone signaling and transduction pathways. Noteworthy, 19 transcription factors (TFs) and 16 hormone signaling pathway-related genes were identified to be predominantly expressed in rhizome bud compared with the other tissues, suggesting putative roles in rhizome formation and development. In addition, a predictive regulatory network was constructed between four TFs and six auxin and abscisic acid (ABA) -related genes. Furthermore, the expression of 24 rhizome-specific genes was further validated by quantitative real-time RT-PCR (qRT-PCR) analysis. Taken together, this study provide a global portrait of gene expression across five different tissues and reveal preliminary insights into rhizome growth and development. The data presented will contribute to our understanding of the molecular mechanisms underlying rhizome development in M. lutarioriparius and remarkably enrich the genomic resources of Miscanthus.

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“…One of the most promising feedstocks is the perennial grass miscanthus due to its high biomass production per hectare with a low environmental impact (Clifton-brown et al, 2004; Lewandowski and Schmidt, 2006; Hastings et al, 2008; Cadoux et al, 2014) and its minor impact on the food supply (Clifton-brown et al, 2004; Heaton et al, 2008; Brosse et al, 2012). The genus Miscanthus comprises 20 species (Hodkinson et al, 2002), including Miscanthus × giganteus, Miscanthus sinensis, Miscanthus sacchariflorus and Miscanthus tinctorius , which are characterized by a high yield and energy content (Cadoux et al, 2014; Lee and Kuan, 2015). The mostly studied genotype in Europe and North America is M .…”

Section: Introductionmentioning

confidence: 99%

Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition

Belmokhtar¹,

Arnoult²,

Chabbert

et al. 2017

Front. Plant Sci.

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HIGHLIGHTS Biomass production and cell wall composition are differentially impacted by harvesting year and genotypes, influencing then cellulose conversion in miniaturized assay.Using a high-throughput miniaturized and semi-automated method for performing the pretreatment and saccharification steps at laboratory scale allows for the assessment of these factors on the biomass potential for producing bioethanol before moving to the industrial scale.The large genetic diversity of the perennial grass miscanthus makes it suitable for producing cellulosic ethanol in biorefineries. The saccharification potential and year variability of five genotypes belonging to Miscanthus × giganteus and Miscanthus sinensis were explored using a miniaturized and semi-automated method, allowing the application of a hot water treatment followed by an enzymatic hydrolysis. The studied genotypes highlighted distinct cellulose conversion yields due to their distinct cell wall compositions. An inter-year comparison revealed significant variations in the biomass productivity and cell wall compositions. Compared to the recalcitrant genotypes, more digestible genotypes contained higher amounts of hemicellulosic carbohydrates and lower amounts of cellulose and lignin. In contrast to hemicellulosic carbohydrates, the relationships analysis between the biomass traits and cellulose conversion clearly showed the same negative effect of cellulose and lignin on cellulose digestion. The miniaturized and semi-automated method we developed was usable at the laboratory scale and was reliable for mimicking the saccharification at the pilot scale using a steam explosion pretreatment and enzymatic hydrolysis. Therefore, this miniaturized method will allow the reliable screening of many genotypes for saccharification potential. These findings provide valuable information and tools for breeders to create genotypes combining high yield, suitable biomass composition, and high saccharification yields.

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Miscanthus as cellulosic biomass for bioethanol production

Cited by 111 publications

References 66 publications

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

Evaluation of different pretreatments of Napier grass for enzymatic saccharification and ethanol production

De novo Transcriptome Analysis of Miscanthus lutarioriparius Identifies Candidate Genes in Rhizome Development

Saccharification Performances of Miscanthus at the Pilot and Miniaturized Assay Scales: Genotype and Year Variabilities According to the Biomass Composition

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