<i>MiR-150-3p</i> targets SP1 and suppresses the growth of glioma cells

Tan, Zhigang; Jia, Jiaoying; Jiang, Yugang

doi:10.1042/bsr20180019

Cited by 19 publications

(10 citation statements)

References 42 publications

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“…After data analyses, a total of 26 miRNAs, 248 mRNAs, 1118 lncRNAs, and 382 circRNAs were differential between miR‐145‐5p mimics and NC groups. We noted both tumor suppressive and oncogenic miRNAs were included in the differentially expressed miRNAs, among which miR‐148b‐3p, miR‐150‐3p, miR‐2116‐3p, miR‐3940‐5p, miR‐4653‐3p, miR‐4701‐5p, miR‐4741, miR‐4749‐3p, miR‐4769‐3p, and miR‐663a are tumor suppressive miRNAs . Moreover, miR‐1539, miR‐1825, miR‐3151‐3p, miR‐550a‐3p, miR‐550a‐5p, and miR‐575 are oncogenic miRNAs .…”

Section: Discussionmentioning

confidence: 95%

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

et al. 2019

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This study intends to investigate the transcriptional regulatory role of miR‐145‐5p in laryngeal squamous cell carcinoma (LSCC). LSCC cell line TU‐177 is transfected with miR‐145‐5p mimics, generating miR‐145‐5p‐overexpression LSCC cells. Whole transcriptome microarrays are used to investigate the differentially expressed lncRNAs, circRNAs, mRNAs, and miRNAs. The target genes of miRNAs are predicted and performed functional annotation. Additionally, the circRNAs, lncRNAs, and mRNAs that interact with miRNAs are predicted, and then the competing endogenous RNAs (ceRNAs) are predicted. Microarray analysis identifies 26 miRNAs, 248 mRNAs, 1118 lncRNAs, and 382 circRNAs differentially expressed in miR‐145‐5p overexpressed LSCC cells. Overall, 675 target genes are identified for the differentially expressed miRNAs, which involved in cell adhesion associated gene ontology (GO) terms, and MAPK and FoxO signaling pathways. The up‐regulated mRNAs involved in the pathway of ABC transporters, while the down‐regulated mRNAs involved in pathway of olfactory transduction. Moreover, 149 ceRNAs are predicted, which are associated with apoptosis, Wnt pathway, and metabolic pathway. Furthermore, qPCR results confirm that miR‐145‐5p affects expression of lncRNAs, miRNAs, mRNAs, and circRNAs in LSCC cells. Collectively, miR‑145‑5p may be inhibits LSCC progression via ceRNA‐mediated pathways, such as WNT2B‐miR‐145‐5p‐NONHSAT127539.2, CASP10‐miR‐145‐5p‐NONHSAT127539.2, CASP10‐miR‐145‐5p‐circ_0003519, and TPO‐miR‐145‐5p‐circ_0003519.

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Section: Discussionmentioning

confidence: 95%

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

et al. 2019

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“…The plasmids expressing miRNAs, which were treated by transient transfection, could remain stable from 0 to 72 h post trVLP infection with the same observation result of GFP under fluorescence microscope [ 37 , 38 ]. As for the miR-150-3p, its stability in cell culture has been confirmed in previous studies [ 39 , 40 ], which means it can provide sustained functions of affecting protein synthesis post trVLP infection. As shown in Figure 2 b, all the plasmid transfection was done 24 h before trVLP infection.…”

Section: Methodsmentioning

confidence: 58%

A Rapid Screen for Host-Encoded miRNAs with Inhibitory Effects against Ebola Virus Using a Transcription- and Replication-Competent Virus-Like Particle System

Wang

et al. 2018

IJMS

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MicroRNAs (miRNAs) may become efficient antiviral agents against the Ebola virus (EBOV) targeting viral genomic RNAs or transcripts. We previously conducted a genome-wide search for differentially expressed miRNAs during viral replication and transcription. In this study, we established a rapid screen for miRNAs with inhibitory effects against EBOV using a tetracistronic transcription- and replication-competent virus-like particle (trVLP) system. This system uses a minigenome comprising an EBOV leader region, luciferase reporter, VP40, GP, VP24, EBOV trailer region, and three noncoding regions from the EBOV genome and can be used to model the life cycle of EBOV under biosafety level (BSL) 2 conditions. Informatic analysis was performed to select up-regulated miRNAs targeting the coding regions of the minigenome with the highest binding energy to perform inhibitory effect screening. Among these miRNAs, miR-150-3p had the most significant inhibitory effect. Reverse transcription polymerase chain reaction (RT-PCR), Western blot, and double fluorescence reporter experiments demonstrated that miR-150-3p inhibited the reproduction of trVLPs via the regulation of GP and VP40 expression by directly targeting the coding regions of GP and VP40. This novel, rapid, and convenient screening method will efficiently facilitate the exploration of miRNAs against EBOV under BSL-2 conditions.

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“…In contrast, miR-150-5p inhibits CD133-positive liver cancer stem cells 35 , functions as a tumor suppressor in colorectal cancer cells 36 , and suppresses triple-negative breast cancer metastasis 37 . In addition, miR-150-3p suppresses the growth of glioma cells by targeting SP1 55 , and induces apoptosis in pancreatic cancer cells 56 . In the present study, we demonstrated that the overexpression of both miR-150-5p and miR-150-3p inhibits OC growth and metastasis in vitro and in vivo, which combined with previous reports 57,58 strongly supports the tumor-suppressive effects of miR-150-5p/3p in OC.…”

Section: Discussionmentioning

confidence: 99%

FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop

et al. 2021

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Ovarian cancer (OC) causes more deaths than any other gynecological cancer. Many cellular pathways have been elucidated to be associated with OC development and progression. Specifically, the insulin-like growth factor 1 receptor/insulin receptor substrate 1 (IGF1R/IRS1) pathway participates in OC development. Moreover, accumulating evidence has shown that microRNA deregulation contributes to tumor initiation and progression. Here, our study aimed to investigate the molecular functions and regulatory mechanisms of miR-150, specifically, in OC. We found that the expression of miR-150-5p/3p and their precursor, mir-150, was downregulated in OC tissues; lower mir-150 levels were associated with poor OC patient outcomes. Ectopic mir-150 expression inhibited OC cell growth and metastasis in vitro and in vivo. Furthermore, both IRS1 and IGF1R were confirmed as direct targets of miR-150-5p/3p, and the miR-150-IGF1R/IRS1 axis exerted antitumor effects via the PI3K/AKT/mTOR pathway. Forkhead box protein 3 (FoxP3) positively regulated the expression of miR-150-5p/3p by binding to the mir-150 promoter. In turn, the PI3K/AKT/mTOR pathway downregulated FoxP3 and miR-150-5p/3p. Taken together, these findings indicate that a complex FoxP3-miR-150-IGF1R/IRS1-PI3K/AKT/mTOR feedback loop regulates OC pathogenesis, providing a novel mechanism for miR-150 as a tumor suppressor miRNA in OC.

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MiR-150-3p targets SP1 and suppresses the growth of glioma cells

Cited by 19 publications

References 42 publications

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

Identification of miR‐145‐5p‐Centered Competing Endogenous RNA Network in Laryngeal Squamous Cell Carcinoma

A Rapid Screen for Host-Encoded miRNAs with Inhibitory Effects against Ebola Virus Using a Transcription- and Replication-Competent Virus-Like Particle System

FoxP3-miR-150-5p/3p suppresses ovarian tumorigenesis via an IGF1R/IRS1 pathway feedback loop

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